IRAK1 Antibody – Mouse Monoclonal

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; lipid particle; endosome membrane; cytosol; nucleus

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; ubiquitin-protein ligase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; protein ubiquitination; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of MAP kinase activity; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; toll-like receptor 4 signaling pathway; aging; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; innate immune response; regulation of cytokine and chemokine mediated signaling pathway; toll-like receptor 9 signaling pathway; negative regulation of apoptosis