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JAM-B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246367

MBS246367 is an antibody targeting JAM-B. Also known as Mouse Monoclonal [clone 1G4] (IgG1,k) to Human JAM-B / JAM2. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
JAM-B / JAM2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1G4] (IgG1,k) to Human JAM-B / JAM2
Product Gene Name
Product Synonym Gene Name
JAM2
Product Synonym Names
Anti-JAM-B / JAM2 Antibody (clone 1G4) IHC-plus; JAM2; C21orf43; JAM-B; JAMB; Junctional adhesion molecule B; JAM-2; JAM-IT/VE-JAM; PRO245; VEJAM; VE-JAM; CD322; CD322 antigen; Junctional adhesion molecule 2; Human JAM-B; JAM2
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1G4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.48 mg/ml (lot specific)
Specificity
Human JAM2
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
34,554 Da
OMIM
606870
3D Structure
ModBase 3D Structure for P57087
Target Species
Human
Immunogen Description
JAM2 (AAH17779, 29 a.a. ~ 299 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
JAM-B / JAM2 antibody was raised against jAM2 (AAH17779, 29 a.a. ~ 299 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-JAM-B antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-JAM-B antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-JAM-B antibody

Anti-JAM2 / JAMB / CD232 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-JAM-B Monoclonal Antibody – Cat# MBS246367

Western Blot (WB) of anti-JAM-B antibody

Western blot of JAM2 expression in LNCaP cells with JAM2 monoclonal antibody, clone 1G4.
Mouse Anti-JAM-B Monoclonal Antibody, Western Blot (WB) – Cat# MBS246367

ELISA (EIA) of anti-JAM-B antibody

Detection limit for recombinant GST tagged JAM2 is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-JAM-B Monoclonal Antibody, ELISA (EIA) – Cat# MBS246367

Other Notes

Small volumes of anti-JAM-B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for JAM-B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
junctional adhesion molecule B isoform 1
NCBI Official Synonym Full Names
junctional adhesion molecule 2
NCBI Official Symbol
JAM2
NCBI Official Synonym Symbols
JAMB; CD322; JAM-B; VEJAM; PRO245; VE-JAM; C21orf43
UniProt Protein Name
Junctional adhesion molecule B
UniProt Synonym Protein Names
Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD_antigen: CD322
UniProt Gene Name
JAM2
UniProt Synonym Gene Names
C21orf43; VEJAM; JAM-B; JAM-2; VE-JAM
UniProt Entry Name
JAM2_HUMAN
NCBI Protein Information
junctional adhesion molecule B; JAM-2; JAM-IT/VE-JAM; vascular endothelial junction-associated molecule

NCBI Summary for JAM-B

This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2012]
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UniProt Comments for JAM-B

JAM2: May play a role in the processes of lymphocyte homing to secondary lymphoid organs. Belongs to the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 21q21.2

Cellular Component: tight junction; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: cell-cell adhesion; extracellular matrix organization and biogenesis; negative regulation of cell adhesion; blood coagulation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JAM-B antibody

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