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RGS3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246368

MBS246368 is an antibody targeting RGS3. Also known as Mouse Monoclonal [clone 1E8-C7] (IgG1,k) to Human RGS3. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
RGS3, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1E8-C7] (IgG1,k) to Human RGS3
Product Gene Name
Product Synonym Names
Anti-RGS3 Antibody (clone 1E8-C7) IHC-plus; RGS3; C2PA; RGP3; PDZ-RGS3; Human RGS3
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1E8-C7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.4 mg/ml (lot specific)
Specificity
Human RGS3
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
66,478 Da
OMIM
602189
3D Structure
ModBase 3D Structure for P49796
Target Species
Human
Immunogen Description
RGS3 (AAH18072, 1 a.a. ~ 193 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
RGS3 antibody was raised against rGS3 (AAH18072, 1 a.a. ~ 193 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-RGS3 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RGS3 antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell line lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-RGS3 antibody

Anti-RGS3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-RGS3 Monoclonal Antibody – Cat# MBS246368

Immunofluorescence (IF) of anti-RGS3 antibody

Immunofluorescence of monoclonal antibody to RGS3 on HeLa cell. [antibody concentration 10 ug/ml].
Mouse Anti-RGS3 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246368

Western Blot (WB) of anti-RGS3 antibody

Western blot of RGS3 expression in transfected 293T cell line by RGS3 monoclonal antibody clone 1E8-C7.
Mouse Anti-RGS3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246368

ELISA (EIA) of anti-RGS3 antibody

Detection limit for recombinant GST tagged RGS3 is 0.3 ng/ml as a capture antibody.
Mouse Anti-RGS3 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246368

Other Notes

Small volumes of anti-RGS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RGS3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
regulator of G-protein signaling 3
NCBI Official Symbol
RGS3
NCBI Official Synonym Symbols
C2PA; RGP3
UniProt Protein Name
Regulator of G-protein signaling 3
UniProt Gene Name
RGS3
UniProt Synonym Gene Names
RGP3; RGS3
UniProt Entry Name
RGS3_HUMAN
NCBI Protein Information
regulator of G-protein signaling 3

NCBI Summary for RGS3

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTPase-activating protein that inhibits G-protein-mediated signal transduction. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear. [provided by RefSeq, Jan 2013]
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UniProt Comments for RGS3

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; inactivation of MAPK activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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