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DRAK1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246381

STK17A, a DAPK type protein kinase, functions in apoptotic signaling. The catalytic domain is similar to that of other members of this subfamily, and the noncatalytic C-terminus is responsible for regulation of kinase activity. In vitro assays have shown that… More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DRAK1 / STK17A, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4D12] (IgG2a,k) to Human DRAK1 / STK17A
Product Gene Name
Product Synonym Gene Name
STK17A
Product Synonym Names
Anti-DRAK1 / STK17A Antibody (clone 4D12) IHC-plus; STK17A; DRAK1; Serine/threonine kinase 17a; Human DRAK1; STK17A
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4D12
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.23 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Molecular Weight
46,558 Da
OMIM
604726
3D Structure
ModBase 3D Structure for Q9UEE5
Target Species
Human
Immunogen Description
STK17A (AAH47696, 301 a.a. ~ 414 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
DRAK1 / STK17A antibody was raised against sTK17A (AAH47696, 301 a.a. ~ 414 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-DRAK1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), RNA interference
Application Notes for anti-DRAK1 antibody
ELISA, IHC-P (5 ug/ml), RNAi, WB
Usage: Western Blot (Cell lysate) - positive control HepG2. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).

Immunohistochemistry (IHC) of anti-DRAK1 antibody

Anti-STK17A / DRAK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-DRAK1 Monoclonal Antibody – Cat# MBS246381

Immunohistochemistry (IHC) of anti-DRAK1 antibody

Anti-STK17A / DRAK1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-DRAK1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246381

Western Blot (WB) of anti-DRAK1 antibody

STK17A monoclonal antibody clone 4D12 Western blot of STK17A expression in HepG2.
Mouse Anti-DRAK1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246381

ELISA (EIA) of anti-DRAK1 antibody

Detection limit for recombinant GST tagged STK17A is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-DRAK1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246381

Other Notes

Small volumes of anti-DRAK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DRAK1 purchase

MBS246381 is an antibody targeting DRAK1. Also known as Mouse Monoclonal [clone 4D12] (IgG2a,k) to Human DRAK1 / STK17A. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DRAK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase 17A
NCBI Official Synonym Full Names
serine/threonine kinase 17a
NCBI Official Symbol
STK17A
NCBI Official Synonym Symbols
DRAK1
UniProt Protein Name
Serine/threonine-protein kinase 17A
UniProt Synonym Protein Names
DAP kinase-related apoptosis-inducing protein kinase 1
UniProt Gene Name
STK17A
UniProt Synonym Gene Names
DRAK1
UniProt Entry Name
ST17A_HUMAN
NCBI Protein Information
serine/threonine-protein kinase 17A; death-associated protein kinase-related 1; serine/threonine kinase 17a (apoptosis-inducing); DAP kinase-related apoptosis-inducing protein kinase 1

NCBI Summary for DRAK1

This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq, Jul 2008]

UniProt Comments for DRAK1

DRAK1: a calmodulin-dependent kinase of the DAPK family. Interacts with and is regulated by calcineurin homologous protein (CHP), an EF-hand Ca(2+)-binding protein. In vitro, CHP inhibits the apoptosis-inducing protein kinase DRAK2 in the presence of elevated Ca(2+) levels. Highly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.

Protein type: Protein kinase, CAMK; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: apoptosis; positive regulation of apoptosis; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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