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PDCD1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246391

PD-1 (PDCD1) encodes a cell surface membrane protein of the immunoglobulin superfamily. PD-1 is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies… More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PDCD1 / CD279 / PD-1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone J116] (IgG1,k) to Human PDCD1 / CD279 / PD-1
Product Gene Name
Product Synonym Gene Name
CD279; PD-1
Product Synonym Names
Anti-PDCD1 / CD279 / PD-1 Antibody (clone J116) IHC-plus; PDCD1; CD279; HPD-1; HPD-l; PD1; Protein PD-1; SLEB2; PD-1; CD279 antigen; Programmed cell death 1; Human PDCD1; CD279; PD-1
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
J116
Host
Mouse
Species Reactivity
Human
Form/Format
Aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer.
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
2-8 degree C.
Molecular Weight
31,647 Da
OMIM
gene 605218
3D Structure
ModBase 3D Structure for Q15116
Target Species
Human
Immunogen Description
Human PDCD1
Immunogen
PDCD1 / CD279 / PD-1 antibody was raised against human PDCD1

Product Categories/Family for anti-PDCD1 antibody

Applications Tested/Suitable for anti-PDCD1 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes for anti-PDCD1 antibody
Flo, IHC-Fr, IHC-P (20 ug/ml), IP, WB
Usage: The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume o...

Immunohistochemistry (IHC) of anti-PDCD1 antibody

Anti-CD279 / PD-1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Mouse Anti-PDCD1 Monoclonal Antibody – Cat# MBS246391

Flow Cytometry (FC/FACS) of anti-PDCD1 antibody

Surface staining of PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Mouse Anti-PDCD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS246391

Flow Cytometry (FC/FACS) of anti-PDCD1 antibody

Surface staining of human PD-1 transfected cells with anti-human PD-1 (J116) FITC. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
Mouse Anti-PDCD1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS246391

Other Notes

Small volumes of anti-PDCD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCD1 purchase

MBS246391 is an antibody targeting PDCD1. Also known as Mouse Monoclonal [clone J116] (IgG1,k) to Human PDCD1 / CD279 / PD-1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PDCD1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
UniProt Protein Name
Programmed cell death protein 1
UniProt Synonym Protein Names
CD_antigen: CD279
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
UniProt Entry Name
PDCD1_HUMAN
NCBI Protein Information
programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2

NCBI Summary for PDCD1

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDCD1

PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: signal transducer activity; protein binding

Biological Process: apoptosis; multicellular organismal development; T cell costimulation; signal transduction; humoral immune response

Disease: Systemic Lupus Erythematosus
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-PDCD1 antibody

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