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BAF60B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246392

MBS246392 is an antibody targeting BAF60B. Also known as Mouse Monoclonal [clone 2F7] (IgG2a,k) to Human BAF60B / SMARCD2. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
BAF60B / SMARCD2, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2F7] (IgG2a,k) to Human BAF60B / SMARCD2
Product Gene Name
Product Synonym Gene Name
SMARCD2
Product Synonym Names
Anti-BAF60B / SMARCD2 Antibody (clone 2F7) IHC-plus; SMARCD2; BAF60B; BRG1-associated factor 60B; CRACD2; PRO2451; Rsc6p; Swp73-like protein; Swi/snf complex 60 kda subunit; Human BAF60B; SMARCD2
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F7
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human SMARCD2
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
55,239 Da
OMIM
601736
3D Structure
ModBase 3D Structure for Q92925
Target Species
Human
Immunogen Description
SMARCD2 (NP_003068, 398 a.a. ~ 475 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
BAF60B / SMARCD2 antibody was raised against sMARCD2 (NP_003068, 398 a.a. ~ 475 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-BAF60B antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-BAF60B antibody
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-BAF60B antibody

Anti-SMARCD2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-BAF60B Monoclonal Antibody – Cat# MBS246392

Immunofluorescence (IF) of anti-BAF60B antibody

Immunofluorescence of monoclonal antibody to SMARCD2 on HeLa cell. [antibody concentration 10 ug/ml]
Mouse Anti-BAF60B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246392

Western Blot (WB) of anti-BAF60B antibody

SMARCD2 monoclonal antibody clone 2F7 Western blot of SMARCD2 expression in HeLa NE.
Mouse Anti-BAF60B Monoclonal Antibody, Western Blot (WB) – Cat# MBS246392

ELISA (EIA) of anti-BAF60B antibody

Detection limit for recombinant GST tagged SMARCD2 is approximately 1 ng/ml as a capture antibody.
Mouse Anti-BAF60B Monoclonal Antibody, ELISA (EIA) – Cat# MBS246392

Other Notes

Small volumes of anti-BAF60B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BAF60B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Synonym Full Names
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
NCBI Official Symbol
SMARCD2
NCBI Official Synonym Symbols
Rsc6p; BAF60B; CRACD2; PRO2451
UniProt Protein Name
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2
UniProt Synonym Protein Names
60 kDa BRG-1/Brm-associated factor subunit B; BRG1-associated factor 60B; BAF60B
UniProt Gene Name
SMARCD2
UniProt Synonym Gene Names
BAF60B; BAF60B
UniProt Entry Name
SMRD2_HUMAN
NCBI Protein Information
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2

NCBI Summary for BAF60B

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for BAF60B

SMARCD2: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the SMARCD family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: SWI/SNF complex; protein complex; nuclear chromatin; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; transcription coactivator activity

Biological Process: regulation of transcription from RNA polymerase II promoter; chromatin remodeling; nucleosome disassembly; transcription, DNA-dependent; ATP-dependent chromatin remodeling
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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