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Nestin (NES) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246397

Nestin (NES) is an intermediate filament protein. Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments. More details

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Product Overview

Product Name
Nestin, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 10C2] (IgG1) to Human Nestin
Product Gene Name
anti-NES antibody
Product Synonym Names
Anti-Nestin Antibody (clone 10C2) IHC-plus; Nestin; Nbla00170; Human Nestin
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10C2
Host
Mouse
Species Reactivity
Human
Form/Format
Aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Purity/Purification
Affinity Purified
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C, avoid repeated freeze thaw cycles.
Molecular Weight
177,439 Da
OMIM
600915
3D Structure
ModBase 3D Structure for P48681
Target Species
Human
Immunogen Description
Human NES
Applications Tested/Suitable for anti-NES antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
Application Notes for anti-NES antibody
IHC-Fr, IHC-P (20 ug/ml), WB
Usage: This 10C2 antibody has been tested by immunoblot analysis of cell line. This can be used at less than or equal to 5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number s...

Immunohistochemistry (IHC) of anti-NES antibody

Anti-Nestin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Mouse Anti-NES Monoclonal Antibody – Cat# MBS246397

Western Blot (WB) of anti-NES antibody

U251 cell lysates prepared under non-reducing (lane 1) or reducing (lane 2) conditions were resolved by SDS-PAGE then immunoblotted with 2 ug/ml of anti-human nestin (10C2) antibody. Bands were visualized using HRP-conjugated anti-mouse IgG.
Mouse Anti-NES Monoclonal Antibody, Western Blot (WB) – Cat# MBS246397

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES purchase

MBS246397 is an antibody targeting NES. Also known as Mouse Monoclonal [clone 10C2] (IgG1) to Human Nestin. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Protein Information
nestin

NCBI Summary for NES

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

UniProt Comments for NES

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; brain development; cell projection morphogenesis; negative regulation of neuron apoptosis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
Show more

Research Articles on NES

1. nestin expression is a novel independent prognostic indicator for patients with UCB

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 EGF antibody  >71 publications with NES and EGF
 BMP4 antibody  >16 publications with NES and BMP4
 MYCN antibody  >14 publications with NES and MYCN
 SOX10 antibody  >14 publications with NES and SOX10
 CDK5 antibody  >13 publications with NES and CDK5
 VSX2 antibody  >6 publications with NES and VSX2
 CDK5R1 antibody  >4 publications with NES and CDK5R1

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >936 publications with NES and Nervous System Diseases
 Brain Diseases Antibodies  >662 publications with NES and Brain Diseases
 Disease Models, Animal Antibodies  >247 publications with NES and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >213 publications with NES and Cardiovascular Diseases
 Seizures Antibodies  >153 publications with NES and Seizures
 Nerve Degeneration Antibodies  >75 publications with NES and Nerve Degeneration
 Breast Neoplasms Antibodies  >71 publications with NES and Breast Neoplasms
 Adenocarcinoma Antibodies  >69 publications with NES and Adenocarcinoma
 Heart Diseases Antibodies  >59 publications with NES and Heart Diseases
 Necrosis Antibodies  >56 publications with NES and Necrosis

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >2087 publications with NES and Nerve
 Brain Antibodies  >1296 publications with NES and Brain
 Embryonic Tissue Antibodies  >701 publications with NES and Embryonic Tissue
 Muscle Antibodies  >252 publications with NES and Muscle
 Vascular Antibodies  >244 publications with NES and Vascular
 Eye Antibodies  >175 publications with NES and Eye
 Skin Antibodies  >143 publications with NES and Skin
 Pancreas Antibodies  >115 publications with NES and Pancreas
 Heart Antibodies  >103 publications with NES and Heart
 Kidney Antibodies  >98 publications with NES and Kidney
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