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TNFRSF18 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246415

MBS246415 is an antibody targeting TNFRSF18. Also known as Mouse Monoclonal [clone 2H4] (IgG2b,k) to Human TNFRSF18 / GITR. Reactive in Human, Mouse. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TNFRSF18 / GITR, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2H4] (IgG2b,k) to Human TNFRSF18 / GITR
Product Gene Name
anti-TNFRSF18 antibody
Product Synonym Gene Name
GITR
Product Synonym Names
TNFRSF18; AITR; CD357; CD357 antigen; GITR; GITR-D
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
2H4
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
PBS, pH 7.4.
Purity/Purification
Purified from ascites by Protein A
Concentration
0.5 mg/ml (lot specific)
Specificity
Human TNFRSF18
Preparation and Storage
Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.
OMIM
603905
3D Structure
ModBase 3D Structure for Q9Y5U5
Family/Subfamily
TNF Receptor/ not assigned-TNF Receptor
Target Species
Human
Immunogen Species
TNFRSF18 / GITR antibody was raised against Human
Immunogen
TNFRSF18 / GITR antibody was raised against recombinant protein encoding amino acids 26-115 of human TNFRSF18
Antigen Type
Recombinant protein
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Note
During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
Warning
This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.

Product Categories/Family for anti-TNFRSF18 antibody

Family: TNF Receptor
Applications Tested/Suitable for anti-TNFRSF18 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TNFRSF18 antibody
ELISA
IHC-P (5 ug/ml)
WB (1:500 - 1:1000)
(optimal dilution to be determined by the researcher)
Usage: Western Blot: Cell lysate, Cell lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-TNFRSF18 antibody

Anti-TNFRSF18 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-TNFRSF18 Monoclonal Antibody – Cat# MBS246415

Western Blot (WB) of anti-TNFRSF18 antibody

TNFRSF18 monoclonal antibody, clone 2H4. Western blot of TNFRSF18 expression in NIH/3T3.
Mouse Anti-TNFRSF18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246415

Western Blot (WB) of anti-TNFRSF18 antibody

TNFRSF18 monoclonal antibody, clone 2H4. Western blot of TNFRSF18 expression in HeLa nuclear extracts.
Mouse Anti-TNFRSF18 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246415

Other Notes

Small volumes of anti-TNFRSF18 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFRSF18. It may not necessarily be applicable to this product.

NCBI GI #
4759246
NCBI GeneID
8784
NCBI Accession #
NP_004186.1
UniProt Primary Accession #
Q9Y5U5
UniProt Secondary Accession #
O95851; Q5U0I4; Q9NYJ9; B1AME1
UniProt Related Accession #
Q9Y5U5
GenBank Nucleotide #
NM_004195.2
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 18 isoform 1
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 18
NCBI Official Symbol
TNFRSF18
NCBI Official Synonym Symbols
AITR; GITR; CD357; GITR-D
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 18
UniProt Synonym Protein Names
Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; CD_antigen: CD357
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
TNFRSF18
UniProt Synonym Gene Names
AITR; GITR
UniProt Entry Name
TNR18_HUMAN
NCBI Protein Information
tumor necrosis factor receptor superfamily member 18; activation-inducible TNFR family receptor; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein

NCBI Summary for TNFRSF18

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]
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UniProt Comments for TNFRSF18

TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction; negative regulation of apoptosis
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Research Articles on TNFRSF18

1. these results show a higher susceptibility to apoptosis in patients' versus controls' T(reg) cells, suggesting that GITR is a T(reg)-cell marker that would be primarily involved in T(reg)-cell survival rather than in their suppressor function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNFRSF18 antibody

 Reference Product  PubMed Publications
 FOXP3 antibody  >132 publications with TNFRSF18 and FOXP3
 CTLA4 antibody  >76 publications with TNFRSF18 and CTLA4
 IL2RA antibody  >68 publications with TNFRSF18 and IL2RA
 TNFSF18 antibody  >51 publications with TNFRSF18 and TNFSF18
 IL2RB antibody  >17 publications with TNFRSF18 and IL2RB
 TRAF2 antibody  >3 publications with TNFRSF18 and TRAF2
 TRAF1 antibody  >2 publications with TNFRSF18 and TRAF1
 IL2RG antibody  >1 publications with TNFRSF18 and IL2RG

Pathways associated with anti-TNFRSF18 antibody

 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram

Diseases associated with anti-TNFRSF18 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >258 publications with TNFRSF18 and Necrosis
 Immune System Diseases Antibodies  >84 publications with TNFRSF18 and Immune System Diseases
 Inflammation Antibodies  >52 publications with TNFRSF18 and Inflammation
 Disease Models, Animal Antibodies  >36 publications with TNFRSF18 and Disease Models, Animal
 Carcinoma Antibodies  >13 publications with TNFRSF18 and Carcinoma
 Neoplasms, Experimental Antibodies  >13 publications with TNFRSF18 and Neoplasms, Experimental
 Liver Diseases Antibodies  >10 publications with TNFRSF18 and Liver Diseases
 Nervous System Diseases Antibodies  >8 publications with TNFRSF18 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >7 publications with TNFRSF18 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >5 publications with TNFRSF18 and Breast Neoplasms

Organs/Tissues associated with anti-TNFRSF18 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >255 publications with TNFRSF18 and Blood
 Lymph Node Antibodies  >31 publications with TNFRSF18 and Lymph Node
 Lung Antibodies  >15 publications with TNFRSF18 and Lung
 Intestine Antibodies  >7 publications with TNFRSF18 and Intestine
 Brain Antibodies  >6 publications with TNFRSF18 and Brain
 Kidney Antibodies  >4 publications with TNFRSF18 and Kidney
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