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MUC16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246428

Also commonly known as Ovarian Cancer Antigen CA-125. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
MUC16 / CA125, Monoclonal Antibody
Full Product Name
Mouse Monoclonal (IgG1) to Human MUC16 / CA125
Product Gene Name
anti-MUC16 antibody
Product Synonym Gene Name
CA125
Product Synonym Names
Anti-MUC16/CA125 Antibody; MUC16; CA125 ovarian cancer antigen; CA125; CA-125; MUC-16; Mucin-16
Clonality
Monoclonal
Isotype
IgG1
Host
Mouse
Species Reactivity
Human
Form/Format
10 mM Tris buffer, pH 7.5, 150 mM sodium chloride, 0.05% sodium azide.
Purity/Purification
Protein A Purified
Concentration
1 mg/ml (lot specific)
Specificity
Recognizes CA 125
Preparation and Storage
Upon arrival: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
2,353,428 Da
OMIM
606154
3D Structure
ModBase 3D Structure for Q8WXI7
Immunogen Species
MUC16/CA125 antibody was raised against Human
Immunogen
MUC16 / CA125 antibody was raised against affinity pure CA 125 antigen (human).
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-MUC16 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-MUC16 antibody
IHC-P (5 ug/ml)
Optimal dilution to be determined by the researcher

Immunohistochemistry (IHC) of anti-MUC16 antibody

Anti-MUC16 / CA-125 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-MUC16 Monoclonal Antibody – Cat# MBS246428

Other Notes

Small volumes of anti-MUC16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MUC16 purchase

MUC16. aka CA 125; mucin 16, is a very large transmembrane hydrophilic protein. As an ovarian cancer marker, cancer antigen antibodies to detect tissue or circulating proteins are common lab tools. MyBiosource's mouse monoclonal anti CA 125 antibody ranks top among products category, with uncharacteristically high reactivity for IHC immunohistochemistry formalin PFA fixed paraffin embedded sections of human tumor cell expressing cancer antigen 125. Our monoclonal antibody is adaptable to western blot with proper loading control and flow cytometry analysis using rabbit secondary antibodies anti mouse IgG Alexa 174 fluor. This product and other species MUC16 antibody products make ideal ELISA pairs for your custom kits as primary antibodies and control reverse pairs. Our resources to obtain the highest grades of antibody production protocols have made our protein family antibodies #1 across the board. View our custom antibody production list and pick your protein labeling kits and done.

Additional Details

NCBI/Uniprot data below describe general gene information for MUC16. It may not necessarily be applicable to this product.

NCBI GI #
83367077
NCBI GeneID
94025
NCBI Accession #
NP_078966.2
UniProt Primary Accession #
Q8WXI7
UniProt Secondary Accession #
Q6ZQW5; Q96RK2
UniProt Related Accession #
Q8WXI7
GenBank Nucleotide #
NM_024690.2
NCBI Official Full Name
mucin-16
NCBI Official Synonym Full Names
mucin 16, cell surface associated
NCBI Official Symbol
MUC16
NCBI Official Synonym Symbols
CA125
UniProt Protein Name
Mucin-16
UniProt Synonym Protein Names
Ovarian cancer-related tumor marker CA125; CA-125; Ovarian carcinoma antigen CA125
Protein Family
Mucin
UniProt Gene Name
MUC16
UniProt Synonym Gene Names
CA125; MUC-16; CA-125
UniProt Entry Name
MUC16_HUMAN
NCBI Protein Information
mucin-16; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125

UniProt Comments for MUC16

MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Protein type: Cell adhesion; Membrane protein, peripheral; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle

Molecular Function: protein binding

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; cell adhesion; post-translational protein modification
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Research Articles on MUC16

1. The modified cutoff value of CA125, HE4, RMI score and ROMA were 165.2 U/mL, 103.4 pM, 368.7, 28/54.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MUC16 antibody

 Reference Product  PubMed Publications
 MUC1 antibody  >82 publications with MUC16 and MUC1
 MUC4 antibody  >55 publications with MUC16 and MUC4
 MUC2 antibody  >18 publications with MUC16 and MUC2
 MUC7 antibody  >10 publications with MUC16 and MUC7
 MUC13 antibody  >10 publications with MUC16 and MUC13
 MUC20 antibody  >7 publications with MUC16 and MUC20
 MUC15 antibody  >7 publications with MUC16 and MUC15
 LGALS1 antibody  >3 publications with MUC16 and LGALS1
 MSLN antibody  >3 publications with MUC16 and MSLN

Pathways associated with anti-MUC16 antibody

 Products by Pathway  Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 O-linked Glycosylation Pathway antibodies  O-linked Glycosylation Pathway Diagram
 O-linked Glycosylation Of Mucins Pathway antibodies  O-linked Glycosylation Of Mucins Pathway Diagram
 Post-translational Protein Modification Pathway antibodies  Post-translational Protein Modification Pathway Diagram
 Termination Of O-glycan Biosynthesis Pathway antibodies  Termination Of O-glycan Biosynthesis Pathway Diagram

Diseases associated with anti-MUC16 antibody

 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >205 publications with MUC16 and Ovarian Neoplasms
 Adenocarcinoma Antibodies  >68 publications with MUC16 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >24 publications with MUC16 and Neoplasm Metastasis
 Inflammation Antibodies  >15 publications with MUC16 and Inflammation
 Gastrointestinal Diseases Antibodies  >15 publications with MUC16 and Gastrointestinal Diseases
 Lung Diseases Antibodies  >14 publications with MUC16 and Lung Diseases
 Skin Diseases Antibodies  >13 publications with MUC16 and Skin Diseases
 Cardiovascular Diseases Antibodies  >11 publications with MUC16 and Cardiovascular Diseases
 Heart Diseases Antibodies  >11 publications with MUC16 and Heart Diseases
 Necrosis Antibodies  >10 publications with MUC16 and Necrosis

Organs/Tissues associated with anti-MUC16 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >214 publications with MUC16 and Blood
 Eye Antibodies  >56 publications with MUC16 and Eye
 Lung Antibodies  >33 publications with MUC16 and Lung
 Ovary Antibodies  >29 publications with MUC16 and Ovary
 Lymph Node Antibodies  >24 publications with MUC16 and Lymph Node
 Epididymis Antibodies  >14 publications with MUC16 and Epididymis
 Uterus Antibodies  >14 publications with MUC16 and Uterus
 Pancreas Antibodies  >7 publications with MUC16 and Pancreas
 Intestine Antibodies  >7 publications with MUC16 and Intestine
 Cervix Antibodies  >6 publications with MUC16 and Cervix
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