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CTSK Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246441

MBS246441 is an antibody targeting CTSK. Also known as Mouse Monoclonal [clone 2F1] (IgG2a,k) to Human CTSK / Cathepsin K. Reactive in Human. Host: Mouse. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CTSK / Cathepsin K, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2F1] (IgG2a,k) to Human CTSK / Cathepsin K
Product Gene Name
anti-CTSK antibody
Product Synonym Names
Anti-CTSK / Cathepsin K Antibody (clone 2F1) IHC-plus; CTSK; Cathepsin K; Cathepsin K (pycnodysostosis); Cathepsin O1; Cathepsin O2; Cathepsin X; CTSO2; PKND; Procathepsin K; PYCD; Cathepsin O; CTS02; CTSO1; Human CTSK; Cathepsin K
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4.
Purity/Purification
Purified from ascites by Protein A
Concentration
0.5 mg/ml (lot specific)
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
OMIM
phenotype 601105
3D Structure
ModBase 3D Structure for P43235
Target Species
Human
Immunogen Type
Recombinant protein
Immunogen
CTSK / Cathepsin K antibody was raised against cTSK (AAH16058, 220 a.a. ~ 331 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CTSK antibody

Family: Protease; Subfamily: Cysteine C1
Applications Tested/Suitable for anti-CTSK antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CTSK antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).

Immunohistochemistry (IHC) of anti-CTSK antibody

Anti-CTSK / Cathepsin K antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CTSK Monoclonal Antibody – Cat# MBS246441

Immunohistochemistry (IHC) of anti-CTSK antibody

Anti-CTSK / Cathepsin K antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CTSK Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246441

Western Blot (WB) of anti-CTSK antibody

Western blot of CTSK expression in transfected 293T cell line by CTSK monoclonal antibody, clone 2F1.
Mouse Anti-CTSK Monoclonal Antibody, Western Blot (WB) – Cat# MBS246441

ELISA (EIA) of anti-CTSK antibody

Detection limit for recombinant GST tagged CTSK is approximately 3 ng/ml as a capture antibody.
Mouse Anti-CTSK Monoclonal Antibody, ELISA (EIA) – Cat# MBS246441

Other Notes

Small volumes of anti-CTSK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CTSK. It may not necessarily be applicable to this product.

NCBI GI #
4503151
NCBI GeneID
1513
NCBI Accession #
NP_000387.1
UniProt Primary Accession #
P43235
UniProt Secondary Accession #
Q6FHS6
UniProt Related Accession #
P43235
GenBank Nucleotide #
NM_000396.3
NCBI Official Full Name
cathepsin K preproprotein
NCBI Official Synonym Full Names
cathepsin K
NCBI Official Symbol
CTSK
NCBI Official Synonym Symbols
CTSO; PKND; PYCD; CTS02; CTSO1; CTSO2
UniProt Protein Name
Cathepsin K
UniProt Synonym Protein Names
Cathepsin O; Cathepsin O2; Cathepsin X
Protein Family
Cathepsin
UniProt Gene Name
CTSK
UniProt Synonym Gene Names
CTSO; CTSO2
UniProt Entry Name
CATK_HUMAN
NCBI Protein Information
cathepsin K; cathepsin O; cathepsin X; cathepsin O1; cathepsin O2

NCBI Summary for CTSK

The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013]
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UniProt Comments for CTSK

CTSK: Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Defects in CTSK are the cause of pycnodysostosis (PKND). PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.38; Protease

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; extracellular region

Molecular Function: collagen binding; proteoglycan binding; protein binding; fibronectin binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: collagen catabolic process; extracellular matrix disassembly; intramembranous ossification; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; toll-like receptor signaling pathway; innate immune response; proteolysis; bone resorption

Disease: Pycnodysostosis
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Research Articles on CTSK

1. Cathepsin K appears to be consistently and strongly expressed in melanocytic lesions and valuable in distinguishing malignant melanomas from the majority of human cancers.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CTSK antibody

 Reference Product  PubMed Publications
 NFATC1 antibody  >44 publications with CTSK and NFATC1
 TLR3 antibody  >1 publications with CTSK and TLR3
 TLR7 antibody  >1 publications with CTSK and TLR7
 TLR9 antibody  >1 publications with CTSK and TLR9

Pathways associated with anti-CTSK antibody

 Products by Pathway  Pathway Diagram
 Activation Of Matrix Metalloproteinases Pathway antibodies  Activation Of Matrix Metalloproteinases Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Collagen Degradation Pathway antibodies  Collagen Degradation Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 Lysosome Pathway antibodies  Lysosome Pathway Diagram
 MHC Class II Antigen Presentation Pathway antibodies  MHC Class II Antigen Presentation Pathway Diagram

Diseases associated with anti-CTSK antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >57 publications with CTSK and Necrosis
 Disease Models, Animal Antibodies  >55 publications with CTSK and Disease Models, Animal
 Pycnodysostosis Antibodies  >52 publications with CTSK and Pycnodysostosis
 Inflammation Antibodies  >44 publications with CTSK and Inflammation
 Cardiovascular Diseases Antibodies  >31 publications with CTSK and Cardiovascular Diseases
 Lung Diseases Antibodies  >22 publications with CTSK and Lung Diseases
 Atherosclerosis Antibodies  >21 publications with CTSK and Atherosclerosis
 Nervous System Diseases Antibodies  >19 publications with CTSK and Nervous System Diseases
 Fibrosis Antibodies  >15 publications with CTSK and Fibrosis
 Breast Neoplasms Antibodies  >15 publications with CTSK and Breast Neoplasms

Organs/Tissues associated with anti-CTSK antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >489 publications with CTSK and Bone
 Blood Antibodies  >134 publications with CTSK and Blood
 Connective Tissue Antibodies  >111 publications with CTSK and Connective Tissue
 Lung Antibodies  >41 publications with CTSK and Lung
 Vascular Antibodies  >29 publications with CTSK and Vascular
 Mouth Antibodies  >28 publications with CTSK and Mouth
 Muscle Antibodies  >23 publications with CTSK and Muscle
 Liver Antibodies  >22 publications with CTSK and Liver
 Heart Antibodies  >16 publications with CTSK and Heart
 Brain Antibodies  >15 publications with CTSK and Brain
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