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FZR1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246501

MBS246501 is an antibody targeting FZR1. Also known as Mouse Monoclonal [clone 4C4] (IgG2a,k) to Human FZR1 / CDH1. Reactive in Human. Host: Mouse. More details

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Product Overview

Product Name
FZR1 / CDH1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 4C4] (IgG2a,k) to Human FZR1 / CDH1
Product Gene Name
anti-FZR1 antibody
Product Synonym Gene Name
CDH1
Product Synonym Names
Anti-FZR1 / CDH1 Antibody (clone 4C4) IHC-plus; FZR1; CDC20-like 1b; CDC20-like protein 1; FYR; FZR; FZR2; HCDH; Fizzy-related protein homolog; KIAA1242; CDC20C; Cdh1/Hct1 homolog; HCDH1; Human FZR1; CDH1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4C4
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human CDH1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
44,874 Da
OMIM
603619
3D Structure
ModBase 3D Structure for Q9UM11
Target Species
Human
Immunogen Description
FZR1 (AAH13413, 1 a.a. ~ 494 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
FZR1 / CDH1 antibody was raised against fZR1 (AAH13413, 1 a.a. ~ 494 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-FZR1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-FZR1 antibody
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using tissue lysates and the recombinant protein used as the immunogen.

Immunohistochemistry (IHC) of anti-FZR1 antibody

Anti-FZR1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-FZR1 Monoclonal Antibody – Cat# MBS246501

Immunohistochemistry (IHC) of anti-FZR1 antibody

Anti-FZR1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-FZR1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246501

Western Blot (WB) of anti-FZR1 antibody

FZR1 monoclonal antibody, clone 4C4. Western blot of FZR1 expression in HeLa NE.
Mouse Anti-FZR1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246501

ELISA (EIA) of anti-FZR1 antibody

Detection limit for recombinant GST tagged FZR1 is 1 ng/ml as a capture antibody.
Mouse Anti-FZR1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246501

Other Notes

Small volumes of anti-FZR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FZR1. It may not necessarily be applicable to this product.

NCBI GI #
33620742
NCBI GeneID
51343
NCBI Accession #
NP_057347.2
UniProt Primary Accession #
Q9UM11
UniProt Secondary Accession #
O75869; Q86U66; Q96NW8; Q9UI96; Q9ULH8; Q9UM10; Q9UNQ1; Q9Y2T8
UniProt Related Accession #
Q9UM11
GenBank Nucleotide #
NM_016263.3
NCBI Official Full Name
fizzy-related protein homolog isoform 2
NCBI Official Synonym Full Names
fizzy/cell division cycle 20 related 1 (Drosophila)
NCBI Official Symbol
FZR1
NCBI Official Synonym Symbols
FZR; CDH1; FZR2; HCDH; HCDH1; CDC20C
UniProt Protein Name
Fizzy-related protein homolog
UniProt Synonym Protein Names
CDC20-like protein 1; Cdh1/Hct1 homolog; hCDH1
Protein Family
Fizzy-related protein
UniProt Gene Name
FZR1
UniProt Synonym Gene Names
CDH1; FYR; FZR; KIAA1242; Fzr; hCDH1
UniProt Entry Name
FZR_HUMAN
NCBI Protein Information
fizzy-related protein homolog; CDC20-like 1b; cdh1/Hct1 homolog; CDC20-like protein 1

UniProt Comments for FZR1

FZR1: Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. The unphosphorylated form interacts with APC/C during mitosis. Interacts with NINL. Interacts (in complex with the anaphase promoting complex APC) with MAD2L2; inhibits FZR1- mediated APC/C activation. Interacts with USP37. Interacts (via WD repeats) with MAK. Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues. Belongs to the WD repeat CDC20/Fizzy family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; nuclear membrane; anaphase-promoting complex; cytosol

Molecular Function: protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of meiosis; DNA repair; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex activation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; positive regulation of protein catabolic process; mitotic cell cycle; G2/M transition DNA damage checkpoint
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Research Articles on FZR1

1. Cdh1 contributes to spatiotemporal organization of AurB activity, and organization of FHOD1 activity by AurB contributes to daughter cell spreading after mitosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FZR1 antibody

 Products by Pathway  Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C Complex Pathway antibodies  APC/C Complex Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Aurora A Signaling Pathway antibodies  Aurora A Signaling Pathway Diagram
 Autodegradation Of Cdh1 By Cdh1:APC/C Pathway antibodies  Autodegradation Of Cdh1 By Cdh1:APC/C Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram

Diseases associated with anti-FZR1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >3 publications with FZR1 and Adenocarcinoma
 Liver Neoplasms Antibodies  >2 publications with FZR1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >2 publications with FZR1 and Disease Models, Animal
 Hyperplasia Antibodies  >2 publications with FZR1 and Hyperplasia
 Liver Diseases Antibodies  >2 publications with FZR1 and Liver Diseases
 Brain Diseases Antibodies  >2 publications with FZR1 and Brain Diseases
 Embryo Loss Antibodies  >2 publications with FZR1 and Embryo Loss
 Carcinoma, Hepatocellular Antibodies  >2 publications with FZR1 and Carcinoma, Hepatocellular
 Infertility, Male Antibodies  >2 publications with FZR1 and Infertility, Male
 Congenital Abnormalities Antibodies  >1 publications with FZR1 and Congenital Abnormalities

Organs/Tissues associated with anti-FZR1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with FZR1 and Brain
 Blood Antibodies  >7 publications with FZR1 and Blood
 Ovary Antibodies  >4 publications with FZR1 and Ovary
 Uterus Antibodies  >4 publications with FZR1 and Uterus
 Embryonic Tissue Antibodies  >3 publications with FZR1 and Embryonic Tissue
 Placenta Antibodies  >3 publications with FZR1 and Placenta
 Bone Antibodies  >3 publications with FZR1 and Bone
 Liver Antibodies  >3 publications with FZR1 and Liver
 Kidney Antibodies  >2 publications with FZR1 and Kidney
 Testis Antibodies  >2 publications with FZR1 and Testis
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