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CD48 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246533

MBS246533 is an antibody targeting CD48. Also known as Mouse Monoclonal [clone 3B8-2C2] (IgG2a,k) to Human CD48. Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CD48, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 3B8-2C2] (IgG2a,k) to Human CD48
Product Gene Name
Product Synonym Names
Anti-CD48 Antibody (clone 3B8-2C2) IHC-plus; CD48; BCM1; BCM1 surface antigen; BLAST; BLAST1; CD48 molecule; MEM-102; MCD48; SLAMF2; CD48 antigen; HCD48; Leukocyte antigen MEM-102; TCT.1; Human CD48
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3B8-2C2
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2
Purity/Purification
Protein A Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human CD48
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
19,429 Da
OMIM
109530
3D Structure
ModBase 3D Structure for P09326
Target Species
Human
Immunogen Description
CD48 (AAH30224, 1 a.a. ~ 170 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
CD48 antibody was raised against cD48 (AAH30224, 1 a.a. ~ 170 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-CD48 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CD48 antibody
ELISA, IHC-P (5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.

Immunohistochemistry (IHC) of anti-CD48 antibody

Anti-CD48 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Mouse Anti-CD48 Monoclonal Antibody – Cat# MBS246533

ELISA (EIA) of anti-CD48 antibody

Detection limit for recombinant GST tagged CD48 is approximately 0.1 ng/ml as a capture antibody.
Mouse Anti-CD48 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246533

Other Notes

Small volumes of anti-CD48 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD48. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
CD48 antigen isoform 1
NCBI Official Synonym Full Names
CD48 molecule
NCBI Official Symbol
CD48
NCBI Official Synonym Symbols
BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
UniProt Protein Name
CD48 antigen
UniProt Synonym Protein Names
B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; SLAM family member 2; SLAMF2; Signaling lymphocytic activation molecule 2; TCT.1; CD_antigen: CD48
Protein Family
UniProt Gene Name
CD48
UniProt Synonym Gene Names
BCM1; BLAST1; SLAMF2
UniProt Entry Name
CD48_HUMAN
NCBI Protein Information
CD48 antigen; TCT.1; BCM1 surface antigen; SLAM family member 2; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); signaling lymphocytic activation molecule 2

NCBI Summary for CD48

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
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UniProt Comments for CD48

CD48: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q21.3-q22

Cellular Component: membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; receptor activity

Biological Process: defense response; blood coagulation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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