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H3F3B Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246657

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is… More details

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Product Overview

Product Name
H3F3B, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 2D7-H1] (IgG1,k) to Human H3F3B
Product Gene Name
anti-H3F3B antibody
Product Synonym Names
Anti-H3F3B Antibody (clone 2D7-H1) IHC-plus; H3F3B; H3.3B; Histone H3.3; Human H3F3B
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2D7-H1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.4
Purity/Purification
Purified from ascites by Prtotein A.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human H3F3B
Preparation and Storage
Store at -20°C. Avoid freeze-that cycles.
OMIM
gene 601128
3D Structure
ModBase 3D Structure for Q6NXT2
Target Species
Human Histone H3.3
Immunogen Description
H3F3B (AAH17558, 1 a.a. ~ 137 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Species
Histone H3.3 antibody was raised against Human.
Antigen Type
Recombinant Protein
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-H3F3B antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-H3F3B antibody
ELISA, IF (10 ug/ml), IHC-P (15 ug/ml), WB
Optimal dilutions to be determined by the researcher.
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-H3F3B antibody

Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 15 ug/ml.
Mouse Anti-H3F3B Monoclonal Antibody – Cat# MBS246657

Immunofluorescence (IF) of anti-H3F3B antibody

Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell. [antibody concentration 10 ug/ml].
Mouse Anti-H3F3B Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS246657

Western Blot (WB) of anti-H3F3B antibody

Western blot of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.
Mouse Anti-H3F3B Monoclonal Antibody, Western Blot (WB) – Cat# MBS246657

Other Notes

Small volumes of anti-H3F3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3F3B purchase

MBS246657 is an antibody targeting H3F3B. Also known as Mouse Monoclonal [clone 2D7-H1] (IgG1,k) to Human H3F3B. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for H3F3B. It may not necessarily be applicable to this product.

NCBI GI #
4885385
NCBI GeneID
3021
NCBI Accession #
NP_005315.1
UniProt Primary Accession #
Q6NXT2
UniProt Secondary Accession #
P06351; P33155; Q5VV55; Q5VV56; Q66I33; Q9V3W4
UniProt Related Accession #
P84243
GenBank Nucleotide #
NM_005324.4
NCBI Official Full Name
histone H3.3
NCBI Official Synonym Full Names
H3 histone, family 3B (H3.3B)
NCBI Official Symbol
H3F3B
NCBI Official Synonym Symbols
H3.3B
UniProt Protein Name
Histone H3.3
UniProt Gene Name
H3F3A
UniProt Synonym Gene Names
H3.3A; H3F3
UniProt Entry Name
H33_HUMAN
NCBI Protein Information
histone H3.3

NCBI Summary for H3F3B

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Jan 2015]
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UniProt Comments for H3F3B

H3F3C: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Hominid-specific H3.5/H3F3C preferentially colocalizes with euchromatin, and it is associated with actively transcribed genes. Belongs to the histone H3 family.

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: nucleosome

Molecular Function: nucleosomal DNA binding; protein heterodimerization activity

Biological Process: positive regulation of cell growth
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Research Articles on H3F3B

1. A remarkable picture of tumor type specificity for histone H3.3 driver alterations emerges, indicating that histone H3.3 residues, mutations and genes have distinct functions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-H3F3B antibody

 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amyloids Pathway antibodies  Amyloids Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cellular Senescence Pathway antibodies  Cellular Senescence Pathway Diagram
 Cellular Responses To Stress Pathway antibodies  Cellular Responses To Stress Pathway Diagram
 Condensation Of Prophase Chromosomes Pathway antibodies  Condensation Of Prophase Chromosomes Pathway Diagram
 DNA Methylation Pathway antibodies  DNA Methylation Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with anti-H3F3B antibody

 Disease Name  Pubmed Publications
 Ovarian Neoplasms Antibodies  >2 publications with H3F3B and Ovarian Neoplasms
 Prostatic Neoplasms Antibodies  >2 publications with H3F3B and Prostatic Neoplasms
 Endocrine System Diseases Antibodies  >1 publications with H3F3B and Endocrine System Diseases
 Hypertension Antibodies  >1 publications with H3F3B and Hypertension
 Breast Neoplasms Antibodies  >1 publications with H3F3B and Breast Neoplasms
 Brain Diseases Antibodies  >1 publications with H3F3B and Brain Diseases
 Infertility, Male Antibodies  >1 publications with H3F3B and Infertility, Male
 Ovarian Diseases Antibodies  >1 publications with H3F3B and Ovarian Diseases
 Infertility Antibodies  >1 publications with H3F3B and Infertility

Organs/Tissues associated with anti-H3F3B antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >3 publications with H3F3B and Testis
 Blood Antibodies  >2 publications with H3F3B and Blood
 Prostate Antibodies  >2 publications with H3F3B and Prostate
 Brain Antibodies  >2 publications with H3F3B and Brain
 Bone Antibodies  >1 publications with H3F3B and Bone
 Adipose Tissue Antibodies  >1 publications with H3F3B and Adipose Tissue
 Ascites Antibodies  >1 publications with H3F3B and Ascites
 Vascular Antibodies  >1 publications with H3F3B and Vascular
 Lung Antibodies  >1 publications with H3F3B and Lung
 Embryonic Tissue Antibodies  >1 publications with H3F3B and Embryonic Tissue
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