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HIP1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246697

The product of this gene is a membrane-associated protein that colocalizes with huntingtin. This protein has similarities to cytoskeleton proteins and its interaction with huntingtin is thought to play a functional role in the cell filament network. Loss of… More details

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Product Overview

Product Name
HIP1, Monoclonal Antibody
Full Product Name
Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human HIP1
Product Gene Name
anti-HIP1 antibody
Product Synonym Names
Anti-HIP1 Antibody (clone 1F12) IHC-plus; HIP1; HIP-1; HIP-I; ILWEQ; Human HIP1
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1F12
Host
Mouse
Species Reactivity
Human
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Purity/Purification
Protein A Purified
Concentration
0.4 mg/ml (lot specific)
Specificity
Human HIP1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
– Da
OMIM
601767
3D Structure
ModBase 3D Structure for O00291
Target Species
Human
Immunogen Description
HIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen
HIP1 antibody was raised against hIP1 (NP_005329, 928 a.a. ~ 1038 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Applications Tested/Suitable for anti-HIP1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
Application Notes for anti-HIP1 antibody
ELISA, IHC-P (10 ug/ml), IP, WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Immunohistochemistry (IHC) of anti-HIP1 antibody

Anti-HIP1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Mouse Anti-HIP1 Monoclonal Antibody – Cat# MBS246697

Western Blot (WB) of anti-HIP1 antibody

Western blot of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody, clone 1F12. Lane 1: HIP1 transfected lysate (116.2 kD). Lane 2: Non-transfected lysate.
Mouse Anti-HIP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246697

Western Blot (WB) of anti-HIP1 antibody

HIP1 monoclonal antibody, clone 1F12 Western blot of HIP1 expression in HeLa.
Mouse Anti-HIP1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS246697

Immunoprecipitation (IP) of anti-HIP1 antibody

Immunoprecipitation of HIP1 transfected lysate using anti-HIP1 monoclonal antibody and Protein A Magnetic Bead immunoblotted with HIP1 rabbit polyclonal antibody.
Mouse Anti-HIP1 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS246697

ELISA (EIA) of anti-HIP1 antibody

Detection limit for recombinant GST tagged HIP1 is approximately 0.3 ng/ml as a capture antibody.
Mouse Anti-HIP1 Monoclonal Antibody, ELISA (EIA) – Cat# MBS246697

Other Notes

Small volumes of anti-HIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIP1 purchase

MBS246697 is an antibody targeting HIP1. Also known as Mouse Monoclonal [clone 1F12] (IgG2a,k) to Human HIP1. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HIP1. It may not necessarily be applicable to this product.

NCBI GI #
38045919
NCBI GeneID
3092
NCBI Accession #
NP_005329.3
UniProt Primary Accession #
O00291
UniProt Secondary Accession #
O00328; Q2TB58; Q8TDL4; B4E3I7; E7ES17
UniProt Related Accession #
O00291
GenBank Nucleotide #
NM_005338.6
NCBI Official Full Name
huntingtin-interacting protein 1 isoform 1
NCBI Official Synonym Full Names
huntingtin interacting protein 1
NCBI Official Symbol
HIP1
NCBI Official Synonym Symbols
HIP-I; ILWEQ
UniProt Protein Name
Huntingtin-interacting protein 1
UniProt Synonym Protein Names
Huntingtin-interacting protein I; HIP-I
Protein Family
High-potential iron-sulfur protein
UniProt Gene Name
HIP1
UniProt Synonym Gene Names
HIP-1; HIP-I
UniProt Entry Name
HIP1_HUMAN
NCBI Protein Information
huntingtin-interacting protein 1; huntingtin-interacting protein I

NCBI Summary for HIP1

The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
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UniProt Comments for HIP1

HIP1: Plays a role in clathrin-mediated endocytosis and trafficking. Involved in regulating AMPA receptor trafficking in the central nervous system in an NMDA-dependent manner. Enhances androgen receptor (AR)-mediated transcription. May act as a proapoptotic protein that induces cell death by acting through the intrinsic apoptosis pathway. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis. May play a functional role in the cell filament networks. May be required for differentiation, proliferation, and/or survival of somatic and germline progenitors. A chromosomal aberration involving HIP1 is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;7)(q33;q11.2) with PDGFRB. The chimeric HIP1-PDGFRB transcript results from an in-frame fusion of the two genes. The reciprocal PDGFRB-HIP1 transcript is not expressed. Belongs to the SLA2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytoskeletal

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; cytoskeleton; clathrin-coated vesicle; membrane; intracellular membrane-bound organelle; cytoplasm; clathrin coated vesicle membrane; nucleus

Molecular Function: protein binding, bridging; clathrin binding; protein binding; structural constituent of cytoskeleton; actin binding; phosphoinositide binding

Biological Process: regulation of apoptosis; caspase activation; clathrin cage assembly; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; endocytosis; cell differentiation; actin cytoskeleton organization and biogenesis; positive regulation of receptor-mediated endocytosis

Disease: Prostate Cancer
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Research Articles on HIP1

1. HIP1-ALK, a novel fusion protein is associated with lung adenocarcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-HIP1 antibody

 Reference Product  PubMed Publications
 HTT antibody  >24 publications with HIP1 and HTT
 HIP1R antibody  >22 publications with HIP1 and HIP1R
 IFT57 antibody  >13 publications with HIP1 and IFT57
 AP2B1 antibody  >1 publications with HIP1 and AP2B1

Pathways associated with anti-HIP1 antibody

 Products by Pathway  Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram
 Huntington's Disease Pathway antibodies  Huntington's Disease Pathway Diagram

Diseases associated with anti-HIP1 antibody

 Disease Name  Pubmed Publications
 Prostatic Diseases Antibodies  >6 publications with HIP1 and Prostatic Diseases
 Prostatic Neoplasms Antibodies  >6 publications with HIP1 and Prostatic Neoplasms
 Lung Diseases Antibodies  >3 publications with HIP1 and Lung Diseases
 Weight Loss Antibodies  >3 publications with HIP1 and Weight Loss
 Neurobehavioral Manifestations Antibodies  >3 publications with HIP1 and Neurobehavioral Manifestations
 Necrosis Antibodies  >2 publications with HIP1 and Necrosis
 Lung Neoplasms Antibodies  >2 publications with HIP1 and Lung Neoplasms
 Skin Neoplasms Antibodies  >2 publications with HIP1 and Skin Neoplasms
 Immune System Diseases Antibodies  >2 publications with HIP1 and Immune System Diseases
 Neoplasms, Experimental Antibodies  >2 publications with HIP1 and Neoplasms, Experimental

Organs/Tissues associated with anti-HIP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >42 publications with HIP1 and Brain
 Nerve Antibodies  >31 publications with HIP1 and Nerve
 Prostate Antibodies  >7 publications with HIP1 and Prostate
 Lung Antibodies  >6 publications with HIP1 and Lung
 Skin Antibodies  >5 publications with HIP1 and Skin
 Liver Antibodies  >5 publications with HIP1 and Liver
 Kidney Antibodies  >4 publications with HIP1 and Kidney
 Embryonic Tissue Antibodies  >3 publications with HIP1 and Embryonic Tissue
 Bone Antibodies  >3 publications with HIP1 and Bone
 Mouth Antibodies  >2 publications with HIP1 and Mouth
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