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NR6A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246731

Germ cell nuclear factor (GCNF), a NR6 Germ Cell Nuclear Factor-Like Receptor, has been shown to affect gene expression in embryogenesis and spermatogenesis and is essential for embryonic survival and normal development. Loss of GCNF causes embryonic… More details

$650 / 0.05 mg +1 FREE 8GB USB
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NR6A1 / GCNF, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human NR6A1 / GCNF
Product Gene Name
anti-NR6A1 antibody
Product Synonym Gene Name
GCNF
Product Synonym Names
NR6A1 Antibody, Germ cell nuclear factor Antibody, HGCNF Antibody, NR61 Antibody, HRTR Antibody, RTR Antibody, Ncnf Antibody, GCNF Antibody, GCNF1 Antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human NR6A1 / GCNF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Upon arrival: Aliquot and store undilute at -20 degree C or below for up to 1 year. can be stored undilted at 4 degree C for up to 1 month. Aovid freeze thaw cycles.
Molecular Weight
53,908 Da
OMIM
602778
3D Structure
ModBase 3D Structure for Q15406
Target
NR6A1/GCNF
Target Species
Nuclear receptor subfamily 6, group A, member 1 (NR6A1)
Immunogen Description
Synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1 / GCNF.
Family/Subfamily
NHR/ NR6 Germ cell nuclear factor-like)
Immunogen Species
NR6A1/GCNF antibody was rasied agianst Human.
Immunogen Type
Synthetic peptide
Immunogen
NR6A1 / GCNF antibody was raised against synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1/GCNF. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig (100%); Platypus (89%); Turkey, Chicken (83%).
Epitope
Ligand-binding Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-NR6A1 antibody

Family: NHR; Subfamily: NR6 Germ cell nuclear factor-like
Applications Tested/Suitable for anti-NR6A1 antibody
IHC - Paraffin
Application Notes for anti-NR6A1 antibody
IHC-P (10 ug/ml)
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-NR6A1 antibody

Anti-NR6A1 / GCNF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-NR6A1 Polyclonal Antibody – Cat# MBS246731

Other Notes

Small volumes of anti-NR6A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR6A1 purchase

MBS246731 is an antibody targeting NR6A1. Also known as Rabbit Polyclonal to Human NR6A1 / GCNF. Reactive in Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NR6A1. It may not necessarily be applicable to this product.

NCBI GI #
53729325
NCBI GeneID
2649
NCBI Accession #
NP_201591.2
UniProt Primary Accession #
Q15406
UniProt Secondary Accession #
O00551; O00603; Q5T6F4; Q8NHQ0; Q92898; Q99802
UniProt Related Accession #
Q15406
GenBank Nucleotide #
NM_033334.3
NCBI Official Full Name
nuclear receptor subfamily 6 group A member 1 isoform 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 6, group A, member 1
NCBI Official Symbol
NR6A1
NCBI Official Synonym Symbols
RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
UniProt Protein Name
Nuclear receptor subfamily 6 group A member 1
UniProt Synonym Protein Names
Germ cell nuclear factor; GCNF; hGCNF; Retinoid receptor-related testis-specific receptor; RTR; hRTR
Protein Family
Nuclear receptor subfamily
UniProt Gene Name
NR6A1
UniProt Synonym Gene Names
GCNF; GCNF; hGCNF; RTR; hRTR
UniProt Entry Name
NR6A1_HUMAN
NCBI Protein Information
nuclear receptor subfamily 6 group A member 1; germ cell nuclear factor variant 1; retinoid receptor-related testis-specific receptor; retinoic acid receptor-related testis-associated receptor

NCBI Summary for NR6A1

This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]
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UniProt Comments for NR6A1

NR6A1: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis. Homodimer. Interacts with UIMC1. Shows highest expression in the germ cells of the adult testis. Belongs to the nuclear hormone receptor family. NR6 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity

Biological Process: cell proliferation; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; gamete generation; positive regulation of transcription from RNA polymerase II promoter; gene expression; spermatogenesis; steroid hormone mediated signaling; negative regulation of transcription from RNA polymerase II promoter
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Research Articles on NR6A1

1. Our results show that cellular levels of NR6A1 are correlated with disease progression in prostate cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NR6A1 antibody

 Reference Product  PubMed Publications
 OCT4 antibody  >12 publications with NR6A1 and OCT4
 NCOR1 antibody  >2 publications with NR6A1 and NCOR1
 DNMT3B antibody  >1 publications with NR6A1 and DNMT3B

Pathways associated with anti-NR6A1 antibody

 Products by Pathway  Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway antibodies  POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway Diagram
 Transcriptional Regulation Of Pluripotent Stem Cells Pathway antibodies  Transcriptional Regulation Of Pluripotent Stem Cells Pathway Diagram

Diseases associated with anti-NR6A1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with NR6A1 and Nervous System Diseases
 Liver Diseases Antibodies  >1 publications with NR6A1 and Liver Diseases
 Skin Diseases Antibodies  >1 publications with NR6A1 and Skin Diseases
 Abnormalities, Drug-Induced Antibodies  >1 publications with NR6A1 and Abnormalities, Drug-Induced
 Liver Neoplasms Antibodies  >1 publications with NR6A1 and Liver Neoplasms
 Nervous System Malformations Antibodies  >1 publications with NR6A1 and Nervous System Malformations
 Weight Loss Antibodies  >1 publications with NR6A1 and Weight Loss

Organs/Tissues associated with anti-NR6A1 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >33 publications with NR6A1 and Testis
 Ovary Antibodies  >16 publications with NR6A1 and Ovary
 Embryonic Tissue Antibodies  >14 publications with NR6A1 and Embryonic Tissue
 Brain Antibodies  >11 publications with NR6A1 and Brain
 Bone Antibodies  >8 publications with NR6A1 and Bone
 Liver Antibodies  >6 publications with NR6A1 and Liver
 Kidney Antibodies  >4 publications with NR6A1 and Kidney
 Placenta Antibodies  >3 publications with NR6A1 and Placenta
 Lung Antibodies  >2 publications with NR6A1 and Lung
 Prostate Antibodies  >2 publications with NR6A1 and Prostate
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