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SIP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246758

MBS246758 is an antibody targeting SIP1. Also known as Rabbit Polyclonal to Human SIP1. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
SIP1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SIP1
Product Gene Name
Product Synonym Gene Name
GEMIN2
Product Synonym Names
Anti-SIP1 Antibody (C-Terminus) IHC-plus; GEMIN2; Component of gems 2; Gem-associated protein 2; SIP1; SIP1-delta; Gemin-2; SMN-interacting protein 1; Human SIP1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SIP1
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
4,826 Da
OMIM
602595
3D Structure
ModBase 3D Structure for O14893
Target Species
Human
Immunogen Description
A 19 amino acid peptide near the carboxy terminus of human SIP1.
Immunogen Type
Synthetic peptide
Immunogen
SIP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SIP1.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-SIP1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SIP1 antibody
ELISA, ICC (4 ug/ml), IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5-1 ug/ml.

Immunohistochemistry (IHC) of anti-SIP1 antibody

Anti-SIP1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody – Cat# MBS246758

Immunohistochemistry (IHC) of anti-SIP1 antibody

Anti-SIP1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246758

Immunohistochemistry (IHC) of anti-SIP1 antibody

Anti-SIP1 antibody IHC of human colon, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246758

Immunofluorescence (IF) of anti-SIP1 antibody

Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS246758

Immunocytochemistry (ICC) of anti-SIP1 antibody

Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS246758

Western Blot (WB) of anti-SIP1 antibody

Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1ug/ml.
Rabbit Anti-SIP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS246758

Other Notes

Small volumes of anti-SIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SIP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gem-associated protein 2 isoform alpha
NCBI Official Synonym Full Names
gem (nuclear organelle) associated protein 2
NCBI Official Symbol
GEMIN2
NCBI Official Synonym Symbols
SIP1; SIP1-delta
UniProt Protein Name
Gem-associated protein 2
UniProt Synonym Protein Names
Component of gems 2; Survival of motor neuron protein-interacting protein 1; SMN-interacting protein 1
UniProt Gene Name
GEMIN2
UniProt Synonym Gene Names
SIP1; Gemin-2; SMN-interacting protein 1
UniProt Entry Name
GEMI2_HUMAN
NCBI Protein Information
gem-associated protein 2; gemin-2; component of gems 2; SMN interacting protein 1-delta; survival of motor neuron protein interacting protein 1

NCBI Summary for SIP1

This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. [provided by RefSeq, Aug 2011]
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UniProt Comments for SIP1

SIP1: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Belongs to the gemin-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleolus; SMN complex; nucleus; cytosol

Molecular Function: protein binding

Biological Process: RNA splicing, via transesterification reactions; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression; mRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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