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15-LOX Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246809

MBS246809 is an antibody targeting 15-LOX. Also known as Goat Polyclonal to Human 15-LOX / ALOX15. Reactive in Gibbon, Gorilla, Human, Monkey, Orangutan. Host: Goat. More details

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Product Overview

Product Name
15-LOX / ALOX15, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human 15-LOX / ALOX15
Product Gene Name
anti-15-LOX antibody
Product Synonym Gene Name
ALOX15
Product Synonym Names
Anti-15-LOX / ALOX15 Antibody (Internal) IHC-plus; ALOX15; 15-lipoxygenase; 15-LOX; 15-lipooxygenase-1; 15-LO-1; 15LOX-1; 15-LOX-1; LOG15; Arachidonate 15-lipoxygenase; Human 15-LOX; ALOX15
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Monkey, Orangutan
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human ALOX15 / 15 Lipoxygenase 1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
70,108 Da
OMIM
152392
3D Structure
ModBase 3D Structure for P16050
Target Species
Human
Immunogen Description
Synthetic peptide C-HYKTDVAVKDDPE from an internal region of human ALOX15 / 15 Lipoxygenase 1 (NP_001131.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Elephant (85%).
Immunogen Type
Synthetic peptide
Immunogen
15-LOX / ALOX15 antibody was raised against synthetic peptide C-HYKTDVAVKDDPE from an internal region of human ALOX15 / 15 Lipoxygenase 1 (NP_001131.3). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Elephant (85%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-15-LOX antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-15-LOX antibody
ELISA (1:32000), IHC-P (2 ug/ml), WB (0.2 - 0.5 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 80kD band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kD according to NP_001131.3). Recommended concentration: 0.2-0.5 ug/ml.

Immunohistochemistry (IHC) of anti-15-LOX antibody

Anti-ALOX15 / 15 Lipoxygenase 1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
Goat Anti-15-LOX Polyclonal Antibody – Cat# MBS246809

Immunohistochemistry (IHC) of anti-15-LOX antibody

Anti-ALOX15 / 15 Lipoxygenase 1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
Goat Anti-15-LOX Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246809

Western Blot (WB) of anti-15-LOX antibody

Antibody (0.2 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-15-LOX Polyclonal Antibody, Western Blot (WB) – Cat# MBS246809

Other Notes

Small volumes of anti-15-LOX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for 15-LOX. It may not necessarily be applicable to this product.

NCBI GI #
40316937
NCBI GeneID
246
NCBI Accession #
NP_001131.3
UniProt Primary Accession #
P16050
UniProt Secondary Accession #
Q8N6R7; Q99657; A8K2P4; B7ZA11
UniProt Related Accession #
P16050
GenBank Nucleotide #
NM_001140.3
NCBI Official Full Name
arachidonate 15-lipoxygenase
NCBI Official Synonym Full Names
arachidonate 15-lipoxygenase
NCBI Official Symbol
ALOX15
NCBI Official Synonym Symbols
12-LOX; 15LOX-1; 15-LOX-1
UniProt Protein Name
Arachidonate 15-lipoxygenase
UniProt Synonym Protein Names
12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (EC:1.13.11.31); 12-LOX; Arachidonate omega-6 lipoxygenase
UniProt Gene Name
ALOX15
UniProt Synonym Gene Names
LOG15; 15-LOX; 15-LOX-1; 12-LOX
UniProt Entry Name
LOX15_HUMAN
NCBI Protein Information
arachidonate 15-lipoxygenase; 15-LOX; 12/15-lipoxygenase; 15-lipooxygenase-1; arachidonate omega-6 lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type

UniProt Comments for 15-LOX

ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.

Protein type: EC 1.13.11.33; EC 1.13.11.31; Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Oxidoreductase; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: extrinsic to internal side of plasma membrane; membrane; plasma membrane; lipid particle; cytosol

Molecular Function: protein binding; arachidonate 12-lipoxygenase activity; phosphatidylinositol-4,5-bisphosphate binding; hepoxilin A3 synthase activity; arachidonate 15-lipoxygenase activity; hepoxilin-epoxide hydrolase activity; iron ion binding

Biological Process: ossification; positive regulation of heterotypic cell-cell adhesion; wound healing; phosphatidylethanolamine biosynthetic process; leukotriene metabolic process; bone mineralization; positive regulation of actin filament polymerization; lipoxygenase pathway; negative regulation of adaptive immune response; arachidonic acid metabolic process; hepoxilin biosynthetic process; inflammatory response; apoptotic cell clearance
Show more

Research Articles on 15-LOX

1. results demonstrate that UTX is implicated in IL-4 mediated transcriptional activation of the ALOX15 gene

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-15-LOX antibody

 Reference Product  PubMed Publications
 ALOX5 antibody  >217 publications with 15-LOX and ALOX5
 PTGS2 antibody  >53 publications with 15-LOX and PTGS2
 ALOX15B antibody  >51 publications with 15-LOX and ALOX15B
 PTGS1 antibody  >27 publications with 15-LOX and PTGS1
 PLA2G4A antibody  >18 publications with 15-LOX and PLA2G4A
 STAT6 antibody  >12 publications with 15-LOX and STAT6
 ALOX12B antibody  >4 publications with 15-LOX and ALOX12B
 CYP2J2 antibody  >2 publications with 15-LOX and CYP2J2

Pathways associated with anti-15-LOX antibody

 Products by Pathway  Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Linoleic Acid Metabolism Pathway antibodies  Linoleic Acid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram

Diseases associated with anti-15-LOX antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >214 publications with 15-LOX and Inflammation
 Atherosclerosis Antibodies  >138 publications with 15-LOX and Atherosclerosis
 Disease Models, Animal Antibodies  >68 publications with 15-LOX and Disease Models, Animal
 Hypertension Antibodies  >37 publications with 15-LOX and Hypertension
 Necrosis Antibodies  >28 publications with 15-LOX and Necrosis
 Kidney Diseases Antibodies  >23 publications with 15-LOX and Kidney Diseases
 Heart Diseases Antibodies  >20 publications with 15-LOX and Heart Diseases
 Fibrosis Antibodies  >16 publications with 15-LOX and Fibrosis
 Arthritis, Rheumatoid Antibodies  >14 publications with 15-LOX and Arthritis, Rheumatoid
 Pain Antibodies  >11 publications with 15-LOX and Pain

Organs/Tissues associated with anti-15-LOX antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >105 publications with 15-LOX and Lung
 Prostate Antibodies  >46 publications with 15-LOX and Prostate
 Trachea Antibodies  >17 publications with 15-LOX and Trachea
 Intestine Antibodies  >16 publications with 15-LOX and Intestine
 Embryonic Tissue Antibodies  >12 publications with 15-LOX and Embryonic Tissue
 Adipose Tissue Antibodies  >11 publications with 15-LOX and Adipose Tissue
 Placenta Antibodies  >11 publications with 15-LOX and Placenta
 Lymph Node Antibodies  >6 publications with 15-LOX and Lymph Node
 Testis Antibodies  >3 publications with 15-LOX and Testis
 Mouth Antibodies  >3 publications with 15-LOX and Mouth
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