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RWDD3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246908

MBS246908 is an antibody targeting RWDD3. Also known as Rabbit Polyclonal to Human RWDD3. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Overview

Product Name
RWDD3, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human RWDD3
Product Gene Name
Product Synonym Names
Anti-RWDD3 Antibody (C-Terminus) IHC-plus; RWDD3; DKFZP566K023; RSUME; RWD domain containing 3; Human RWDD3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human RWDD3
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
22,857 Da
OMIM
615875
3D Structure
ModBase 3D Structure for Q9Y3V2
Target Species
Human
Immunogen Description
A 15 amino acid peptide from near the carboxy terminus of human RWDD3.
Immunogen Type
Synthetic peptide
Immunogen
RWDD3 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human RWDD3.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-RWDD3 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RWDD3 antibody
ELISA, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1-2 ug/ml.

Immunohistochemistry (IHC) of anti-RWDD3 antibody

Anti-RWDD3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RWDD3 Polyclonal Antibody – Cat# MBS246908

Immunohistochemistry (IHC) of anti-RWDD3 antibody

Anti-RWDD3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-RWDD3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246908

Western Blot (WB) of anti-RWDD3 antibody

Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-RWDD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS246908

Western Blot (WB) of anti-RWDD3 antibody

Western blot of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Rabbit Anti-RWDD3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS246908

Other Notes

Small volumes of anti-RWDD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RWDD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RWD domain-containing protein 3 isoform a
NCBI Official Synonym Full Names
RWD domain containing 3
NCBI Official Symbol
RWDD3
NCBI Official Synonym Symbols
RSUME
UniProt Protein Name
RWD domain-containing protein 3
UniProt Synonym Protein Names
RWD domain-containing sumoylation enhancer; RSUME
UniProt Gene Name
RWDD3
UniProt Synonym Gene Names
RSUME; RSUME
UniProt Entry Name
RWDD3_HUMAN
NCBI Protein Information
RWD domain-containing protein 3; RWD-containing sumoylation enhancer; RWD domain-containing sumoylation enhancer

UniProt Comments for RWDD3

RWDD3: Enhances the sumoylation of a number of proteins including HIF1A, PIAS and I-kappa-B, through direct interaction with UBC9. Has no effect on ubiquitination. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: SUMO conjugating system

Chromosomal Location of Human Ortholog: 1p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of protein sumoylation; inhibition of NF-kappaB transcription factor
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-RWDD3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1 publications with RWDD3 and Brain
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