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MAD2L1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246913

MBS246913 is an antibody targeting MAD2L1. Also known as Rabbit Polyclonal to Human MAD2L1 / MAD2. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
MAD2L1 / MAD2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human MAD2L1 / MAD2
Product Gene Name
anti-MAD2L1 antibody
Product Synonym Gene Name
MAD2
Product Synonym Names
Anti-MAD2L1 / MAD2 Antibody IHC-plus; MAD2L1; HSMAD2; MAD2; MAD2-like protein 1; Human MAD2L1; MAD2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human Mad2L1. Predicted cross-reactivity based on amino acid sequence homology: mouse (94%), rat (95%).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
10,335 Da
OMIM
601467
3D Structure
ModBase 3D Structure for Q13257
Target Species
Human
Immunogen Description
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 174 of Human MAD2.
Immunogen Type
Recombinant protein
Immunogen
MAD2L1 / MAD2 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 174 of Human MAD2.
Applications Tested/Suitable for anti-MAD2L1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-MAD2L1 antibody
IHC-P (10 ug/ml), WB (1:500 - 1:3000)

Immunohistochemistry (IHC) of anti-MAD2L1 antibody

Anti-MAD2L1 antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MAD2L1 Polyclonal Antibody – Cat# MBS246913

Immunohistochemistry (IHC) of anti-MAD2L1 antibody

Anti-MAD2L1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-MAD2L1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS246913

Western Blot (WB) of anti-MAD2L1 antibody

Sample (30 ug of whole cell lysate). A: 293T. 12% SDS PAGE. MAD2L1 antibody diluted at 1:1000.
Rabbit Anti-MAD2L1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS246913

Other Notes

Small volumes of anti-MAD2L1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MAD2L1. It may not necessarily be applicable to this product.

NCBI GI #
4505067
NCBI GeneID
4085
NCBI Accession #
NP_002349.1
UniProt Primary Accession #
Q13257
UniProt Secondary Accession #
Q53F56; Q548X9; Q6IRW7; Q8IZX3
UniProt Related Accession #
Q13257
GenBank Nucleotide #
NM_002358.3
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD2A
NCBI Official Synonym Full Names
MAD2 mitotic arrest deficient-like 1 (yeast)
NCBI Official Symbol
MAD2L1
NCBI Official Synonym Symbols
MAD2; HSMAD2
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD2A
UniProt Synonym Protein Names
Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1
Protein Family
Mitotic spindle assembly checkpoint protein
UniProt Gene Name
MAD2L1
UniProt Synonym Gene Names
MAD2; HsMAD2; MAD2-like protein 1
UniProt Entry Name
MD2L1_HUMAN
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1

NCBI Summary for MAD2L1

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]
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UniProt Comments for MAD2L1

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis
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Research Articles on MAD2L1

1. Results showed that neither variants in BUB3 nor variants in MAD2L1 caused any significant effect on the risk of breast cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAD2L1 antibody

 Reference Product  PubMed Publications
 CDC20 antibody  >100 publications with MAD2L1 and CDC20
 MAD1L1 antibody  >55 publications with MAD2L1 and MAD1L1
 BUB1B antibody  >24 publications with MAD2L1 and BUB1B
 TTK antibody  >18 publications with MAD2L1 and TTK
 MAD2L1BP antibody  >17 publications with MAD2L1 and MAD2L1BP
 CDC16 antibody  >1 publications with MAD2L1 and CDC16
 ANAPC2 antibody  >1 publications with MAD2L1 and ANAPC2
 CDC27 antibody  >1 publications with MAD2L1 and CDC27

Pathways associated with anti-MAD2L1 antibody

 Products by Pathway  Pathway Diagram
 APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies  APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies  APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway antibodies  Amplification Of Signal From Unattached Kinetochores Via A MAD2 Inhibitory Signal Pathway Diagram
 Amplification Of Signal From The Kinetochores Pathway antibodies  Amplification Of Signal From The Kinetochores Pathway Diagram
 Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway antibodies  Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Checkpoints Pathway antibodies  Cell Cycle Checkpoints Pathway Diagram

Diseases associated with anti-MAD2L1 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >30 publications with MAD2L1 and Adenocarcinoma
 Cell Transformation, Neoplastic Antibodies  >20 publications with MAD2L1 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >20 publications with MAD2L1 and Breast Neoplasms
 Ovarian Neoplasms Antibodies  >13 publications with MAD2L1 and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >11 publications with MAD2L1 and Ovarian Diseases
 Liver Diseases Antibodies  >11 publications with MAD2L1 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >11 publications with MAD2L1 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >10 publications with MAD2L1 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >10 publications with MAD2L1 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >9 publications with MAD2L1 and Neoplasm Metastasis

Organs/Tissues associated with anti-MAD2L1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >15 publications with MAD2L1 and Lung
 Stomach Antibodies  >12 publications with MAD2L1 and Stomach
 Liver Antibodies  >11 publications with MAD2L1 and Liver
 Blood Antibodies  >11 publications with MAD2L1 and Blood
 Bone Antibodies  >10 publications with MAD2L1 and Bone
 Prostate Antibodies  >7 publications with MAD2L1 and Prostate
 Skin Antibodies  >6 publications with MAD2L1 and Skin
 Bone Marrow Antibodies  >6 publications with MAD2L1 and Bone Marrow
 Lymph Antibodies  >5 publications with MAD2L1 and Lymph
 Kidney Antibodies  >5 publications with MAD2L1 and Kidney
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