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INHA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246943

MBS246943 is an antibody targeting INHA. Also known as Rabbit Polyclonal (IgG) to Human INHA / Inhibin Alpha. Reactive in Human. Host: Rabbit. More details

$700 / 0.25 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
INHA / Inhibin Alpha, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human INHA / Inhibin Alpha
Product Gene Name
anti-INHA antibody
Product Synonym Names
Anti-INHA / Inhibin Alpha Antibody IHC-plus; INHA; A-inhibin subunit; Inhibin alpha; Inhibin; alpha; Inhibin alpha chain; Human INHA; Inhibin Alpha
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
10 mM PBS, pH 7.4, BSA, sodium azide.
Purity/Purification
Immunoaffinity Purified
Preparation and Storage
Store at 2 degree C to 8 degree C degrees. Do not freeze.
Molecular Weight
39,670 Da
OMIM
147380
3D Structure
ModBase 3D Structure for P05111
Family/Subfamily
TGF beta/ not assigned-TGF beta
Target Species
Human
Immunogen Description
Synthetic peptide corresponding to human inhibin alpha.
Immunogen Type
Synthetic peptide
Immunogen
INHA / Inhibin Alpha antibody was raised against synthetic peptide corresponding to human inhibin alpha.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-INHA antibody

Family: TGF beta
Applications Tested/Suitable for anti-INHA antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-INHA antibody
IHC-P (1:50)

Immunohistochemistry (IHC) of anti-INHA antibody

Anti-INHA / Inhibin Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
Rabbit Anti-INHA Polyclonal Antibody – Cat# MBS246943

Other Notes

Small volumes of anti-INHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for INHA. It may not necessarily be applicable to this product.

NCBI GI #
4504697
NCBI GeneID
3623
NCBI Accession #
NP_002182.1
UniProt Primary Accession #
P05111
UniProt Secondary Accession #
A8K8H5
UniProt Related Accession #
P05111
GenBank Nucleotide #
NM_002191.3
NCBI Official Full Name
inhibin alpha chain preproprotein
NCBI Official Synonym Full Names
inhibin, alpha
NCBI Official Symbol
INHA
UniProt Protein Name
Inhibin alpha chain
Protein Family
Inhibin
UniProt Gene Name
INHA
UniProt Entry Name
INHA_HUMAN
NCBI Protein Information
inhibin alpha chain; A-inhibin subunit

NCBI Summary for INHA

This gene encodes the alpha subunit of inhibins A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.[provided by RefSeq, Dec 2010]
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UniProt Comments for INHA

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; cell soma; extracellular region

Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: negative regulation of B cell differentiation; hemoglobin biosynthetic process; nervous system development; regulation of cell cycle; male gonad development; signal transduction; negative regulation of interferon-gamma biosynthetic process; regulation of cell proliferation; negative regulation of follicle-stimulating hormone secretion; negative regulation of cell cycle; regulation of apoptosis; response to external stimulus; cell surface receptor linked signal transduction; negative regulation of macrophage differentiation; cell-cell signaling; ovarian follicle development; regulation of MAPKKK cascade; erythrocyte differentiation; negative regulation of phosphorylation; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell cycle arrest; cell development; skeletal development
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Research Articles on INHA

1. When two variant alleles of inhibin alpha gene promoter are combined, the c.-16C>T and c.-124A>G polymorphisms are associated with increased primary ovarian insufficiency risk in Korean women.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-INHA antibody

 Reference Product  PubMed Publications
 INHBA antibody  >19 publications with INHA and INHBA
 INHBB antibody  >17 publications with INHA and INHBB
 CGA antibody  >9 publications with INHA and CGA
 FST antibody  >6 publications with INHA and FST
 FSHB antibody  >4 publications with INHA and FSHB
 TGFBR3 antibody  >3 publications with INHA and TGFBR3
 INHBC antibody  >1 publications with INHA and INHBC
 INHBE antibody  >1 publications with INHA and INHBE
 ACVR2A antibody  >1 publications with INHA and ACVR2A

Pathways associated with anti-INHA antibody

 Products by Pathway  Pathway Diagram
 Glycoprotein Hormones Pathway antibodies  Glycoprotein Hormones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Ovarian Infertility Genes Pathway antibodies  Ovarian Infertility Genes Pathway Diagram
 Peptide Hormone Biosynthesis Pathway antibodies  Peptide Hormone Biosynthesis Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram

Diseases associated with anti-INHA antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >225 publications with INHA and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >194 publications with INHA and Cardiovascular Diseases
 Pain Antibodies  >143 publications with INHA and Pain
 Mental Disorders Antibodies  >85 publications with INHA and Mental Disorders
 Necrosis Antibodies  >63 publications with INHA and Necrosis
 Heart Diseases Antibodies  >61 publications with INHA and Heart Diseases
 Liver Diseases Antibodies  >61 publications with INHA and Liver Diseases
 Hypertension Antibodies  >56 publications with INHA and Hypertension
 Genital Diseases, Male Antibodies  >55 publications with INHA and Genital Diseases, Male
 Liver Neoplasms Antibodies  >32 publications with INHA and Liver Neoplasms

Organs/Tissues associated with anti-INHA antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >187 publications with INHA and Brain
 Lung Antibodies  >116 publications with INHA and Lung
 Liver Antibodies  >114 publications with INHA and Liver
 Eye Antibodies  >70 publications with INHA and Eye
 Ovary Antibodies  >41 publications with INHA and Ovary
 Thyroid Antibodies  >22 publications with INHA and Thyroid
 Testis Antibodies  >17 publications with INHA and Testis
 Uterus Antibodies  >8 publications with INHA and Uterus
 Placenta Antibodies  >5 publications with INHA and Placenta
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