Rabbit Anti-MAP3K3 Polyclonal Antibody – Cat# MBS246954

Anti-MAP3K3 / MEKK3 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

MAP3K3 Antibody – Rabbit Polyclonal

Name: MAP3K3 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS246954
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS246954

MEKK3, a MAP3K type protein kinase, is a member of several MAP kinase signaling pathways. It regulates both JNK and ERK cascades by phosphorylating and activating MKK4/MAP2K4 and MEK1/MAP2K1, respectively. MEKK3 regulates the ERK5/MAPK7 cascade by… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

MAP3K3 / MEKK3, Polyclonal Antibody

Full Product Name

Rabbit Polyclonal to Mouse MAP3K3 / MEKK3

Product Gene Name

anti-MAP3K3 antibody

Product Synonym Gene Name

MEKK3

Product Synonym Names

Anti-MAP3K3 / MEKK3 Antibody IHC-plus; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MEK kinase 3; MEKK3; MAP/ERK kinase kinase 3; MEKK 3; Mouse MAP3K3; MEKK3

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity)

Form/Format

Antiserum

Purity/Purification

Unpurified serum

Specificity

MUNC-18a Antibody detects endogenous levels of total MUNC-18a protein.

Preparation and Storage

Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Molecular Weight

74,089 Da

OMIM

602539

3D Structure

ModBase 3D Structure for Q99759

Target Species

Mouse

Immunogen Description

A 17 residue synthetic peptide DEQEALDSIMKDLVALQ(C) based on the mouse MekK3 (residues 2-18) with the cysteine (C) residue added and the peptide coupled to KLH.

Immunogen Type

Synthetic peptide - KLH conjugated

Immunogen

MAP3K3 / MEKK3 antibody was raised against a 17 residue synthetic peptide DEQEALDSIMKDLVALQ(C) based on the mouse MekK3 (residues 2-18) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat, Elephant (100%); Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (94%); Human, Gorilla, Gibbon, Monkey (88%); Stickleback, Pufferfish (82%).

Product Categories/Family for anti-MAP3K3 antibody

Family: Protein Kinase; Subfamily: MAP3K

Applications Tested/Suitable for anti-MAP3K3 antibody

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

Application Notes for anti-MAP3K3 antibody

IHC-P (1:100), WB (1:5000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Other Notes

Small volumes of anti-MAP3K3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP3K3 purchase

MBS246954 is an antibody targeting MAP3K3. Also known as Rabbit Polyclonal to Mouse MAP3K3 / MEKK3. Reactive in Human, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP3K3. It may not necessarily be applicable to this product.

NCBI GI #

42794765

NCBI GeneID

4215

NCBI Accession #

NP_002392.2

UniProt Primary Accession #

Q99759

UniProt Secondary Accession #

Q5BKZ6; Q8N3I9; B2RCW2; D3DU15

UniProt Related Accession #

Q99759

GenBank Nucleotide #

NM_002401.3

NCBI Official Full Name

mitogen-activated protein kinase kinase kinase 3 isoform 2

NCBI Official Synonym Full Names

mitogen-activated protein kinase kinase kinase 3

NCBI Official Symbol

MAP3K3

NCBI Official Synonym Symbols

MEKK3; MAPKKK3

UniProt Protein Name

Mitogen-activated protein kinase kinase kinase 3

UniProt Synonym Protein Names

MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3

UniProt Gene Name

MAP3K3

UniProt Synonym Gene Names

MAPKKK3; MEKK3; MEK kinase 3; MEKK 3

UniProt Entry Name

M3K3_HUMAN

NCBI Protein Information

mitogen-activated protein kinase kinase kinase 3; MEKK 3; MEK kinase 3; MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3

NCBI Summary for MAP3K3

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

UniProt Comments for MAP3K3

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Protein kinase, STE; EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: intracellular; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade

Research Articles on MAP3K3

1. Inhibitors of apoptosis proteins regulate myogenic differentiation by directly suppressing MEKK2/3-MEK5-ERK5 signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP3K3 antibody

Reference Product	PubMed Publications
IKBKB antibody	>8 publications with MAP3K3 and IKBKB
MAP2K5 antibody	>5 publications with MAP3K3 and MAP2K5
TRAF6 antibody	>4 publications with MAP3K3 and TRAF6
RIPK1 antibody	>3 publications with MAP3K3 and RIPK1
MAP3K7 antibody	>2 publications with MAP3K3 and MAP3K7
CCM2 antibody	>2 publications with MAP3K3 and CCM2
RAC1 antibody	>1 publications with MAP3K3 and RAC1
TAB1 antibody	>1 publications with MAP3K3 and TAB1

Pathways associated with anti-MAP3K3 antibody

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
EBV LMP1 Signaling Pathway antibodies	EBV LMP1 Signaling Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
GnRH Signaling Pathway antibodies	GnRH Signaling Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
HTLV-I Infection Pathway antibodies	HTLV-I Infection Pathway Diagram
IL1-mediated Signaling Events Pathway antibodies	IL1-mediated Signaling Events Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Insulin Signaling Pathway antibodies	Insulin Signaling Pathway Diagram

Diseases associated with anti-MAP3K3 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>20 publications with MAP3K3 and Necrosis
Inflammation Antibodies	>10 publications with MAP3K3 and Inflammation
Breast Neoplasms Antibodies	>4 publications with MAP3K3 and Breast Neoplasms
Cardiovascular Diseases Antibodies	>3 publications with MAP3K3 and Cardiovascular Diseases
Immune System Diseases Antibodies	>3 publications with MAP3K3 and Immune System Diseases
Ovarian Neoplasms Antibodies	>2 publications with MAP3K3 and Ovarian Neoplasms
Nervous System Diseases Antibodies	>2 publications with MAP3K3 and Nervous System Diseases
Ovarian Diseases Antibodies	>2 publications with MAP3K3 and Ovarian Diseases
Esophageal Neoplasms Antibodies	>2 publications with MAP3K3 and Esophageal Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with MAP3K3 and Carcinoma, Hepatocellular

Organs/Tissues associated with anti-MAP3K3 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>19 publications with MAP3K3 and Blood
Vascular Antibodies	>11 publications with MAP3K3 and Vascular
Embryonic Tissue Antibodies	>4 publications with MAP3K3 and Embryonic Tissue
Kidney Antibodies	>4 publications with MAP3K3 and Kidney
Muscle Antibodies	>4 publications with MAP3K3 and Muscle
Bone Antibodies	>4 publications with MAP3K3 and Bone
Brain Antibodies	>4 publications with MAP3K3 and Brain
Bone Marrow Antibodies	>3 publications with MAP3K3 and Bone Marrow
Liver Antibodies	>2 publications with MAP3K3 and Liver
Skin Antibodies	>2 publications with MAP3K3 and Skin