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NDUFAF2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247011

MBS247011 is an antibody targeting NDUFAF2. Also known as Rabbit Polyclonal to Human NDUFAF2 / NDUFA12L. Reactive in Human. Host: Rabbit. More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
NDUFAF2 / NDUFA12L, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human NDUFAF2 / NDUFA12L
Product Gene Name
Product Synonym Gene Name
NDUFA12L
Product Synonym Names
Anti-NDUFAF2 / NDUFA12L Antibody (C-Terminus) IHC-plus; NDUFAF2; B17.2-like; B17.2L; Mimitin; mitochondrial; MMTN; NDUFA12-like protein; NDUFA12L; Mimitin; NDUFA12-like; Human NDUFAF2; NDUFA12L
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human NDUFAF2
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
19,856 Da
OMIM
phenotype 609653
3D Structure
ModBase 3D Structure for Q8N183
Target Species
Human
Immunogen Description
A 14 amino acid peptide from near the carboxy terminus of human Mimitin.
Immunogen Type
Synthetic peptide
Immunogen
NDUFAF2 / NDUFA12L antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Mimitin.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-NDUFAF2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-NDUFAF2 antibody
ELISA, IF, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: Mimitin antibody can be used for detection of Mimitin by Western blot at 1-2 ug/ml.

Immunohistochemistry (IHC) of anti-NDUFAF2 antibody

Anti-NDUFAF2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-NDUFAF2 Polyclonal Antibody – Cat# MBS247011

Immunohistochemistry (IHC) of anti-NDUFAF2 antibody

Anti-NDUFAF2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-NDUFAF2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247011

Immunofluorescence (IF) of anti-NDUFAF2 antibody

Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 ug/ml.
Rabbit Anti-NDUFAF2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS247011

Western Blot (WB) of anti-NDUFAF2 antibody

Western blot of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-NDUFAF2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247011

Other Notes

Small volumes of anti-NDUFAF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NDUFAF2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mimitin, mitochondrial
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) complex I, assembly factor 2
NCBI Official Symbol
NDUFAF2
NCBI Official Synonym Symbols
MMTN; B17.2L; mimitin; NDUFA12L
UniProt Protein Name
Mimitin, mitochondrial
UniProt Synonym Protein Names
B17.2-like; B17.2L; Myc-induced mitochondrial protein; MMTN; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein
Protein Family
UniProt Gene Name
NDUFAF2
UniProt Synonym Gene Names
NDUFA12L; B17.2L; MMTN
UniProt Entry Name
MIMIT_HUMAN
NCBI Protein Information
mimitin, mitochondrial; NDUFA12-like protein; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2

NCBI Summary for NDUFAF2

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency. [provided by RefSeq, Jul 2008]
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UniProt Comments for NDUFAF2

NDUFAF2: Acts as a molecular chaperone for mitochondrial complex I assembly. Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA12 subunit family.

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: mitochondrion

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NDUFAF2 antibody

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