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PPHLN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247056

MBS247056 is an antibody targeting PPHLN1. Also known as Rabbit Polyclonal (IgG) to Human PPHLN1. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PPHLN1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human PPHLN1
Product Gene Name
Product Synonym Names
Anti-PPHLN1 Antibody (aa71-120) IHC-plus; PPHLN1; Gastric cancer antigen Ga50; HSPC232; HSPC206; Periphilin 1; Periphilin-1; Human PPHLN1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
PPHLN Antibody detects endogenous levels of total PPHLN protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
40,319 Da
OMIM
608150
3D Structure
ModBase 3D Structure for Q8NEY8
Target Species
Human
Immunogen Description
Synthetic peptide from human PPHLN (aa71-120).
Immunogen
PPHLN1 antibody was raised against synthetic peptide from human PPHLN (aa71-120).
Antigen Modification
aa71-120
Applications Tested/Suitable for anti-PPHLN1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-PPHLN1 antibody
ELISA (1:5000), IHC-P (10 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-PPHLN1 antibody

Anti-PPHLN1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Rabbit Anti-PPHLN1 Polyclonal Antibody – Cat# MBS247056

Western Blot (WB) of anti-PPHLN1 antibody

Western blot of extracts from COLO/293 cells, using PPHLN Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-PPHLN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247056

Other Notes

Small volumes of anti-PPHLN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PPHLN1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
periphilin-1 isoform 1
NCBI Official Synonym Full Names
periphilin 1
NCBI Official Symbol
PPHLN1
NCBI Official Synonym Symbols
HSPC206; HSPC232
UniProt Protein Name
Periphilin-1
UniProt Synonym Protein Names
Gastric cancer antigen Ga50
Protein Family
UniProt Gene Name
PPHLN1
UniProt Entry Name
PPHLN_HUMAN
NCBI Protein Information
periphilin-1; gastric cancer antigen Ga50

NCBI Summary for PPHLN1

The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
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UniProt Comments for PPHLN1

PPHLN1: Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; RNA-binding

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; cytoplasm

Biological Process: keratinization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PPHLN1 antibody

Organs/Tissues associated with anti-PPHLN1 antibody

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