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UBE1DC1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247097

MBS247097 is an antibody targeting UBE1DC1. Also known as Rabbit Polyclonal (IgG) to Human UBE1DC1 / UBA5. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
UBE1DC1 / UBA5, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human UBE1DC1 / UBA5
Product Gene Name
Product Synonym Gene Name
UBA5
Product Synonym Names
Anti-UBE1DC1 / UBA5 Antibody (aa281-330) IHC-plus; UBA5; THIFP1; Ubiquitin-activating enzyme 5; UBE1DC1; UFM1-activating enzyme; Human UBE1DC1; UBA5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
UBA5 Antibody detects endogenous levels of total UBA5 protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
38,537 Da
OMIM
610552
3D Structure
ModBase 3D Structure for Q9GZZ9
Target Species
Human
Immunogen Description
Synthetic peptide from human UBA5 (aa281-330).
Immunogen
UBE1DC1 / UBA5 antibody was raised against synthetic peptide from human UBA5 (aa281-330).
Antigen Modification
aa281-330
Applications Tested/Suitable for anti-UBE1DC1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-UBE1DC1 antibody
ELISA (1:5000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-UBE1DC1 antibody

Anti-UBA5 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-UBE1DC1 Polyclonal Antibody – Cat# MBS247097

Western Blot (WB) of anti-UBE1DC1 antibody

Western blot of extracts from HepG2/293 cells, using UBA5 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-UBE1DC1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247097

Other Notes

Small volumes of anti-UBE1DC1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UBE1DC1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme 5 isoform 1
NCBI Official Synonym Full Names
ubiquitin-like modifier activating enzyme 5
NCBI Official Symbol
UBA5
NCBI Official Synonym Symbols
THIFP1; UBE1DC1
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme 5
UniProt Synonym Protein Names
ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1
UniProt Gene Name
UBA5
UniProt Synonym Gene Names
UBE1DC1; Ubiquitin-activating enzyme 5
UniProt Entry Name
UBA5_HUMAN
NCBI Protein Information
ubiquitin-like modifier-activating enzyme 5; UFM1-activating enzyme; ubiquitin-activating enzyme 5; UBA5, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-domain containing 1; ubiquitin-activating enzyme E1 domain-containing protein 1

NCBI Summary for UBE1DC1

This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A pseudogene located on chromosome 1 has also been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for UBE1DC1

UBE1DC1: E1-like enzyme which activates UFM1 and SUMO2. Belongs to the ubiquitin-activating E1 family. UBA5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; metal ion binding; ATP binding

Biological Process: regulation of estrogen receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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