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CYSLT2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247163

MBS247163 is an antibody targeting CYSLT2. Also known as Rabbit Polyclonal to Human CYSLT2 / CYSLTR2. Reactive in Human. Host: Rabbit. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CYSLT2 / CYSLTR2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human CYSLT2 / CYSLTR2
Product Gene Name
anti-CYSLT2 antibody
Product Synonym Gene Name
CYSLTR2
Product Synonym Names
Anti-CYSLT2 / CYSLTR2 Antibody (N-Terminus) IHC-plus; CYSLTR2; CysLT(2); CYSLT2; CYSLT2R; CysLT-2; HG57; HGPCR21; KPG_011; PSEC0146; HPN321; Human CYSLT2; CYSLTR2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human CYSLTR2 / CYSLT2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
OMIM
605666
3D Structure
ModBase 3D Structure for Q9NS75
Immunogen Species
CYSLT2 / CYSLTR2 antibody was raised against Human
Antigen Type
Synthetic peptide
Immunogen
CYSLT2 / CYSLTR2 antibody was raised against synthetic 15 amino acid peptide from N-Terminus of human CYSLTR2 / CYSLT2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Horse (80%).
Epitope
N-Terminus
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CYSLT2 antibody

Family: GPCR; Subfamily: Chemoattractant
Applications Tested/Suitable for anti-CYSLT2 antibody
Immunohistochemistry - Paraffin (IHC - P)
Application Notes for anti-CYSLT2 antibody
IHC - P (10 ug/ml); Optimal dilution to be determined by the researcher.

Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment with Proteinase K.

Immunohistochemistry (IHC) of anti-CYSLT2 antibody

Anti-CYSLTR2 / CYSLT2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CYSLT2 Polyclonal Antibody – Cat# MBS247163

Immunohistochemistry (IHC) of anti-CYSLT2 antibody

Anti-CYSLTR2 / CYSLT2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CYSLT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247163

Immunohistochemistry (IHC) of anti-CYSLT2 antibody

Anti-CYSLTR2 / CYSLT2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CYSLT2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247163

Other Notes

Small volumes of anti-CYSLT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CYSLT2. It may not necessarily be applicable to this product.

NCBI GI #
9966851
NCBI GeneID
57105
NCBI Accession #
NP_065110.1
UniProt Primary Accession #
Q9NS75
UniProt Secondary Accession #
Q9HCQ2
UniProt Related Accession #
Q9NS75
GenBank Nucleotide #
NM_020377.2
NCBI Official Full Name
cysteinyl leukotriene receptor 2
NCBI Official Synonym Full Names
cysteinyl leukotriene receptor 2
NCBI Official Symbol
CYSLTR2
NCBI Official Synonym Symbols
HG57; CYSLT2; HPN321; CYSLT2R; KPG_011; hGPCR21; PSEC0146
UniProt Protein Name
Cysteinyl leukotriene receptor 2
UniProt Synonym Protein Names
G-protein coupled receptor GPCR21; hGPCR21; G-protein coupled receptor HG57; HPN321
UniProt Gene Name
CYSLTR2
UniProt Synonym Gene Names
CYSLT2; CYSLT2R; CysLTR2; hGPCR21
UniProt Entry Name
CLTR2_HUMAN
NCBI Protein Information
cysteinyl leukotriene receptor 2; G protein-coupled receptor; G-protein coupled receptor HG57; G-protein coupled receptor GPCR21; cysteinyl leukotriene CysLT2 receptor

NCBI Summary for CYSLT2

The cysteinyl leukotrienes LTC4, LTD4, and LTE4 are important mediators of human bronchial asthma. Pharmacologic studies have determined that cysteinyl leukotrienes activate at least 2 receptors, the protein encoded by this gene and CYSLTR1. This encoded receptor is a member of the superfamily of G protein-coupled receptors. It seems to play a major role in endocrine and cardiovascular systems. [provided by RefSeq, Jul 2008]
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UniProt Comments for CYSLT2

CYSLTR2: Receptor for cysteinyl leukotrienes. The response is mediated via a G-protein that activates a phosphatidylinositol- calcium second messenger system. Stimulation by BAY u9773, a partial agonist, induces specific contractions of pulmonary veins and might also have an indirect role in the relaxation of the pulmonary vascular endothelium. The rank order of affinities for the leukotrienes is LTC4 = LTD4 >> LTE4. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; cysteinyl leukotriene receptor activity; leukotriene receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; immune response
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Research Articles on CYSLT2

1. Suggest that endothelial and nonendothelial CysLT2R niches have separate roles in mediating inflammatory responses in ischemic/perfusion injury.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CYSLT2 antibody

 Reference Product  PubMed Publications
 CYSLTR1 antibody  >15 publications with CYSLT2 and CYSLTR1
 TBXA2R antibody  >3 publications with CYSLT2 and TBXA2R
 LTB4R antibody  >1 publications with CYSLT2 and LTB4R
 GPR17 antibody  >1 publications with CYSLT2 and GPR17

Pathways associated with anti-CYSLT2 antibody

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eicosanoid Ligand-binding Receptors Pathway antibodies  Eicosanoid Ligand-binding Receptors Pathway Diagram
 Endothelins Pathway antibodies  Endothelins Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram

Diseases associated with anti-CYSLT2 antibody

 Disease Name  Pubmed Publications
 Asthma Antibodies  >18 publications with CYSLT2 and Asthma
 Lung Diseases, Obstructive Antibodies  >15 publications with CYSLT2 and Lung Diseases, Obstructive
 Inflammation Antibodies  >7 publications with CYSLT2 and Inflammation
 Rhinitis Antibodies  >4 publications with CYSLT2 and Rhinitis
 Drug Hypersensitivity Antibodies  >3 publications with CYSLT2 and Drug Hypersensitivity
 Asthma, Aspirin-Induced Antibodies  >3 publications with CYSLT2 and Asthma, Aspirin-Induced
 Eosinophilia Antibodies  >2 publications with CYSLT2 and Eosinophilia
 Urticaria Antibodies  >2 publications with CYSLT2 and Urticaria
 Pain Antibodies  >1 publications with CYSLT2 and Pain
 Cardiovascular Diseases Antibodies  >1 publications with CYSLT2 and Cardiovascular Diseases

Organs/Tissues associated with anti-CYSLT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Placenta Antibodies  >1 publications with CYSLT2 and Placenta
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