Goat Anti-POU2AF1 Polyclonal Antibody – Cat# MBS247173

Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

POU2AF1 Antibody – Goat Polyclonal

Name: POU2AF1 Antibody; Polyclonal; Goat; Human/Bovine-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS247173
Price: 625 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247173

MBS247173 is an antibody targeting POU2AF1. Also known as Goat Polyclonal to Human POU2AF1 / BOB1. Reactive in Bat, Chimpanzee, Gorilla, Horse, Human, Monkey, OrangutanPredicted Reactivity: Gibbon, Bovine, Dog (at least 90% immunogen sequence identity). Host:… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

POU2AF1 / BOB1, Polyclonal Antibody

Full Product Name

Goat Polyclonal to Human POU2AF1 / BOB1

Product Gene Name

anti-POU2AF1 antibody

Product Synonym Gene Name

BOB1

Product Synonym Names

Anti-POU2AF1 / BOB1 Antibody (C-Terminus) IHC-plus; POU2AF1; BOB-1; OBF-1; OCT-binding factor 1; BOB1; OBF1; OCA-B; OCAB; Human POU2AF1; BOB1

Clonality

Polyclonal

Host

Goat

Species Reactivity

Bat, Chimpanzee, Gorilla, Horse, Human, Monkey, Orangutan
Predicted Reactivity: Gibbon, Bovine, Dog (at least 90% immunogen sequence identity)

Form/Format

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Purity/Purification

Immunoaffinity Purified

Concentration

0.5 mg/ml (lot specific)

Specificity

Human POU2AF1 / BOB1.

Preparation and Storage

Store at -20 degree C. Minimize freezing and thawing.

Molecular Weight

27,436 Da

OMIM

601206

3D Structure

ModBase 3D Structure for Q16633

Target Species

Human

Immunogen Description

Synthetic peptide C-DSDAYALNHTLSVE from the C-terminus of human POU2AF1 / BOB1 (NP_006226.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin, Bat, Horse (100%); Gibbon, Panda, Dog, Bovine (93%).

Immunogen Type

Synthetic peptide

Immunogen

POU2AF1 / BOB1 antibody was raised against synthetic peptide C-DSDAYALNHTLSVE from the C-terminus of human POU2AF1 / BOB1 (NP_006226.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin, Bat, Horse (100%); Gibbon, Panda, Dog, Bovine (93%); Elephant, Rabbit (86%).

Antigen Modification

C-Terminus

Applications Tested/Suitable for anti-POU2AF1 antibody

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

Application Notes for anti-POU2AF1 antibody

ELISA (1:8000), IHC-P (3.75 ug/ml), WB (1 - 2 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kD was observed. No bands were observed in mock-transfected H...

Other Notes

Small volumes of anti-POU2AF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU2AF1. It may not necessarily be applicable to this product.

NCBI GI #

167900478

NCBI GeneID

5450

NCBI Accession #

NP_006226.2

UniProt Primary Accession #

Q16633

UniProt Secondary Accession #

Q14983; B2R8Z9

UniProt Related Accession #

Q16633; AAB34937

GenBank Nucleotide #

NM_006235.2

NCBI Official Full Name

POU domain class 2-associating factor 1

NCBI Official Synonym Full Names

POU class 2 associating factor 1

NCBI Official Symbol

POU2AF1

NCBI Official Synonym Symbols

BOB1; OBF1; OCAB; OBF-1

UniProt Protein Name

POU domain class 2-associating factor 1

UniProt Synonym Protein Names

B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1

Protein Family

POU domain class 2-associating factor

UniProt Gene Name

POU2AF1

UniProt Synonym Gene Names

OBF1

UniProt Entry Name

OBF1_HUMAN

NCBI Protein Information

POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1

UniProt Comments for POU2AF1

POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.

Protein type: Transcription, coactivator/corepressor; Oncoprotein

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response

Research Articles on POU2AF1

1. It has been identified as a new disease susceptibility gene among Japanese. Though different from Europeans, it is indicated that a B lymphocyte differentiation route shares a common disease developing process. [Review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POU2AF1 antibody

Reference Product	PubMed Publications
POU2F2 antibody	>38 publications with POU2AF1 and POU2F2
POU2F1 antibody	>27 publications with POU2AF1 and POU2F1
PAX5 antibody	>17 publications with POU2AF1 and PAX5
TNFRSF17 antibody	>8 publications with POU2AF1 and TNFRSF17
CD79B antibody	>7 publications with POU2AF1 and CD79B
CXCR5 antibody	>3 publications with POU2AF1 and CXCR5
LGALS1 antibody	>1 publications with POU2AF1 and LGALS1
SIAH1 antibody	>1 publications with POU2AF1 and SIAH1

Diseases associated with anti-POU2AF1 antibody

Disease Name	Pubmed Publications
Lymphoma Antibodies	>36 publications with POU2AF1 and Lymphoma
Leukemia Antibodies	>9 publications with POU2AF1 and Leukemia
Neoplasms, Experimental Antibodies	>5 publications with POU2AF1 and Neoplasms, Experimental
Necrosis Antibodies	>5 publications with POU2AF1 and Necrosis
Liver Diseases Antibodies	>5 publications with POU2AF1 and Liver Diseases
Kidney Diseases Antibodies	>4 publications with POU2AF1 and Kidney Diseases
Cardiovascular Diseases Antibodies	>4 publications with POU2AF1 and Cardiovascular Diseases
Lung Neoplasms Antibodies	>2 publications with POU2AF1 and Lung Neoplasms
Skin Neoplasms Antibodies	>2 publications with POU2AF1 and Skin Neoplasms
Fibrosis Antibodies	>1 publications with POU2AF1 and Fibrosis

Organs/Tissues associated with anti-POU2AF1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>104 publications with POU2AF1 and Blood
Lymph Node Antibodies	>19 publications with POU2AF1 and Lymph Node
Spleen Antibodies	>13 publications with POU2AF1 and Spleen
Lymph Antibodies	>9 publications with POU2AF1 and Lymph
Stomach Antibodies	>2 publications with POU2AF1 and Stomach
Lung Antibodies	>2 publications with POU2AF1 and Lung
Brain Antibodies	>2 publications with POU2AF1 and Brain
Thymus Antibodies	>1 publications with POU2AF1 and Thymus
Cervix Antibodies	>1 publications with POU2AF1 and Cervix
Tonsil Antibodies	>1 publications with POU2AF1 and Tonsil