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IRX3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247250

MBS247250 is an antibody targeting IRX3. Also known as Rabbit Polyclonal (IgG) to Human IRX3. Reactive in Human, Mouse. Host: Rabbit. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
IRX3, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human IRX3
Product Gene Name
Product Synonym Names
Anti-IRX3 Antibody (aa271-320) IHC-plus; IRX3; Homeodomain protein IRXB1; IRXB1; Iroquois homeobox 3; Iroquois homeobox protein 3; IRX-1; Human IRX3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
IRX3 Antibody detects endogenous levels of total IRX3 protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
52,119 Da
OMIM
612985
3D Structure
ModBase 3D Structure for P78415
Target Species
Human
Immunogen Description
Synthetic peptide from human IRX3 (aa271-320).
Immunogen
IRX3 antibody was raised against synthetic peptide from human IRX3 (aa271-320).
Antigen Modification
aa271-320
Applications Tested/Suitable for anti-IRX3 antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-IRX3 antibody
ELISA (1:20000), IHC-P (5 ug/ml)

Immunohistochemistry (IHC) of anti-IRX3 antibody

Anti-IRX3 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-IRX3 Polyclonal Antibody – Cat# MBS247250

Other Notes

Small volumes of anti-IRX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IRX3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
iroquois-class homeodomain protein IRX-3
NCBI Official Synonym Full Names
iroquois homeobox 3
NCBI Official Symbol
IRX3
NCBI Official Synonym Symbols
IRX-1; IRXB1
UniProt Protein Name
Iroquois-class homeodomain protein IRX-3
UniProt Synonym Protein Names
Homeodomain protein IRXB1; Iroquois homeobox protein 3
UniProt Gene Name
IRX3
UniProt Synonym Gene Names
IRXB1
UniProt Entry Name
IRX3_HUMAN
NCBI Protein Information
iroquois-class homeodomain protein IRX-3; homeodomain protein IRXB1; iroquois homeobox protein 3; iroquois-class homeodomain protein IRX-1

NCBI Summary for IRX3

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step of neural development (Bellefroid et al., 1998 [PubMed 9427753]). Members of this family appear to play multiple roles during pattern formation of vertebrate embryos (Lewis et al., 1999 [PubMed 10370142]).[supplied by OMIM, Aug 2009]
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UniProt Comments for IRX3

IRX3: Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. Belongs to the TALE/IRO homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: axon; cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: negative regulation of neuron differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; mesoderm development; positive regulation of neuron differentiation; metanephros development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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