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PLN Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247330

MBS247330 is an antibody targeting PLN. Also known as Goat Polyclonal to Human PLN / Phospholamban. Reactive in Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit. Host: PLN/ Phospholamban antibody was produced in Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PLN / Phospholamban, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human PLN / Phospholamban
Product Gene Name
anti-PLN antibody
Product Synonym Names
Anti-PLN / Phospholamban Antibody (N-Terminus) IHC-plus; PLN; CMD1P; CMH18; PLB; Cardiac phospholamban; Phospholamban; Human PLN; Phospholamban
Clonality
Polyclonal
Host
PLN/ Phospholamban antibody was produced in Goat
Species Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affininty chromotography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human PLN/ Phospholamban
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
OMIM
phenotype 613874
3D Structure
ModBase 3D Structure for P26678
Immunogen Species
PLN/ Phospholamban antibody was raised against Human
Immunogen
PLN/ Phospholamban antibody was rasied against peptide with sequence KVQYLTRSAIRR-C, from the N-Terminus of protein sequence according to NP_002658.1).
Epitope
aa3-14
Presentation
TBS, pH 7.3, 0.02 Sodium Azide, 0.5% BSA
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-PLN antibody
Immunohistochemistry (IHC)
Immunohistochemistry-Paraffin (IHC-P)
Western Blot (WB)
ELISA (EIA)
Peptide Enzyme-Linked Immunosorbent Assay
Application Notes for anti-PLN antibody
IHC - Paraffin (3.75 ug/ml)
Western blot (0.01 - 0.03 ug/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
(Optimal dilution to be determined by the researcher)

Usage Summary: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 13kD band observed in Human and Rat Heart lysates (calculated MW of 6.11kD according to NP_002658.1). Recommended concentration: 0.01-0.03 ug/ml.

Other Notes

Small volumes of anti-PLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PLN. It may not necessarily be applicable to this product.

NCBI GI #
4505887
NCBI GeneID
5350
NCBI Accession #
NP_002658.1
UniProt Primary Accession #
P26678
UniProt Related Accession #
P26678
GenBank Nucleotide #
NM_002667.3
NCBI Official Full Name
cardiac phospholamban
NCBI Official Synonym Full Names
phospholamban
NCBI Official Symbol
PLN
NCBI Official Synonym Symbols
PLB; CMD1P; CMH18
UniProt Protein Name
Cardiac phospholamban
Protein Family
Cardiac phospholamban
UniProt Gene Name
PLN
UniProt Synonym Gene Names
PLB; PLB
UniProt Entry Name
PPLA_HUMAN
NCBI Protein Information
cardiac phospholamban

NCBI Summary for PLN

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]
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UniProt Comments for PLN

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: sarcoplasmic reticulum membrane; mitochondrion; membrane; perinuclear region of cytoplasm; endoplasmic reticulum; mitochondrial membrane; vesicle

Molecular Function: enzyme inhibitor activity; identical protein binding; protein binding; ATPase inhibitor activity; calcium channel regulator activity; ATPase binding

Biological Process: cardiac muscle development; negative regulation of calcium ion transport; negative regulation of heart rate; cytosolic calcium ion homeostasis; blood circulation; regulation of calcium ion transport; response to testosterone stimulus; regulation of heart contraction; response to insulin stimulus; response to zinc ion; calcium ion transport; negative regulation of catalytic activity; negative regulation of ATPase activity; protein homooligomerization; regulation of the force of heart contraction

Disease: Cardiomyopathy, Dilated, 1p; Cardiomyopathy, Familial Hypertrophic, 18
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Research Articles on PLN

1. SLN and PLN are co-expressed in most fibers, which suggests that super-inhibition of SERCAs may be physiologically important in the regulation of intracellular Ca2+ in human skeletal muscle.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PLN antibody

 Reference Product  PubMed Publications
 ATP2A2 antibody  >124 publications with PLN and ATP2A2
 SLN antibody  >23 publications with PLN and SLN
 CASQ2 antibody  >5 publications with PLN and CASQ2
 ATP2A1 antibody  >3 publications with PLN and ATP2A1
 DMPK antibody  >2 publications with PLN and DMPK

Pathways associated with anti-PLN antibody

 Products by Pathway  Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Ion Channel Transport Pathway antibodies  Ion Channel Transport Pathway Diagram
 Ion Transport By P-type ATPases Pathway antibodies  Ion Transport By P-type ATPases Pathway Diagram

Diseases associated with anti-PLN antibody

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >113 publications with PLN and Heart Diseases
 Heart Failure Antibodies  >71 publications with PLN and Heart Failure
 Inflammation Antibodies  >63 publications with PLN and Inflammation
 Disease Models, Animal Antibodies  >60 publications with PLN and Disease Models, Animal
 Cardiomyopathies Antibodies  >50 publications with PLN and Cardiomyopathies
 Kidney Diseases Antibodies  >35 publications with PLN and Kidney Diseases
 Adenocarcinoma Antibodies  >35 publications with PLN and Adenocarcinoma
 Hypertrophy Antibodies  >35 publications with PLN and Hypertrophy
 Vascular Diseases Antibodies  >33 publications with PLN and Vascular Diseases
 Cardiomegaly Antibodies  >33 publications with PLN and Cardiomegaly

Organs/Tissues associated with anti-PLN antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >214 publications with PLN and Muscle
 Heart Antibodies  >210 publications with PLN and Heart
 Vascular Antibodies  >82 publications with PLN and Vascular
 Kidney Antibodies  >46 publications with PLN and Kidney
 Pancreas Antibodies  >36 publications with PLN and Pancreas
 Skin Antibodies  >32 publications with PLN and Skin
 Liver Antibodies  >29 publications with PLN and Liver
 Lung Antibodies  >29 publications with PLN and Lung
 Intestine Antibodies  >28 publications with PLN and Intestine
 Brain Antibodies  >24 publications with PLN and Brain
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