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CXCR3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247354

MBS247354 is an antibody targeting CXCR3. Also known as Rabbit Polyclonal (IgG) to Human CXCR3. Reactive in Human. Host: Rabbit. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CXCR3, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human CXCR3
Product Gene Name
anti-CXCR3 antibody
Product Synonym Names
Anti-CXCR3 Antibody (aa161-210) IHC-plus; CXCR3; C-x-c chemokine receptor 3; CD183 antigen; CKR-L2; CXCR-3; CMKAR3; CD182; CD183; Cxcr3a; GPR9; IP-10 receptor; IP10-R; Mig receptor; MigR; Mig-R; Chemokine (C-X-C) receptor 3; CXC-R3; Cxcr3b; G protein-coupled receptor 9; IP10 receptor; Human CXCR3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
CXCR3 Antibody detects endogenous levels of total CXCR3 protein.
Preparation and Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Molecular Weight
Western Blot: Predicted MW 40 kDa
OMIM
300574
3D Structure
ModBase 3D Structure for P49682
Family/Subfamily
GPCR/Chemokine
Immunogen Species
CXCR3 antibody was raised against Human
Immunogen
CXCR3 antibody was raised against synthetic peptide from human CXCR3. (AA Range: 161-210)
Epitope
aa161-210
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CXCR3 antibody

Family: GPCR; Subfamily: Chemokine
Applications Tested/Suitable for anti-CXCR3 antibody
Immunohistochemistry (IHC) - Paraffin, Western Blot (WB), Peptide ELISA (EIA)
Application Notes for anti-CXCR3 antibody
IHC - Paraffin (1:100)
Western Blot (1:500-1:1000)
Peptide ELISA (1:5000)
(Optimal dilution to be determined by the researcher.)

Other Notes

Small volumes of anti-CXCR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CXCR3. It may not necessarily be applicable to this product.

NCBI GI #
4504099
NCBI GeneID
2833
NCBI Accession #
NP_001495.1
UniProt Primary Accession #
P49682
UniProt Secondary Accession #
O15185; Q7Z710; Q9P2T4; Q9P2T5; B2R982
UniProt Related Accession #
P49682
GenBank Nucleotide #
NM_001504.1
NCBI Official Full Name
C-X-C chemokine receptor type 3 isoform 1
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 3
NCBI Official Symbol
CXCR3
NCBI Official Synonym Symbols
GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
UniProt Protein Name
C-X-C chemokine receptor type 3
UniProt Synonym Protein Names
CKR-L2; G protein-coupled receptor 9; Interferon-inducible protein 10 receptor; IP-10 receptor; CD_antigen: CD183
Protein Family
C-X-C chemokine receptor
UniProt Gene Name
CXCR3
UniProt Synonym Gene Names
GPR9; CXC-R3; CXCR-3; IP-10 receptor
UniProt Entry Name
CXCR3_HUMAN
NCBI Protein Information
C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor

NCBI Summary for CXCR3

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]
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UniProt Comments for CXCR3

CXCR3: Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: C-X-C chemokine binding; chemokine binding; chemokine receptor activity; receptor activity; C-X-C chemokine receptor activity

Biological Process: apoptosis; positive regulation of chemotaxis; calcium-mediated signaling; positive regulation of cAMP metabolic process; chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; negative regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; negative regulation of endothelial cell proliferation; regulation of leukocyte migration; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; cell motility; cell adhesion
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Research Articles on CXCR3

1. plays a role in insulin resistance and obesity-induced visceral adipose inflammation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CXCR3 antibody

 Reference Product  PubMed Publications
 CXCL10 antibody  >815 publications with CXCR3 and CXCL10
 CD4 antibody  >628 publications with CXCR3 and CD4
 CXCL9 antibody  >466 publications with CXCR3 and CXCL9
 CXCL11 antibody  >301 publications with CXCR3 and CXCL11
 CCL5 antibody  >146 publications with CXCR3 and CCL5
 CXCL13 antibody  >28 publications with CXCR3 and CXCL13
 CCL11 antibody  >25 publications with CXCR3 and CCL11
 PF4 antibody  >14 publications with CXCR3 and PF4
 CCL7 antibody  >10 publications with CXCR3 and CCL7
 CCL13 antibody  >3 publications with CXCR3 and CCL13

Pathways associated with anti-CXCR3 antibody

 Products by Pathway  Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram

Diseases associated with anti-CXCR3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >573 publications with CXCR3 and Inflammation
 Autoimmune Diseases Antibodies  >313 publications with CXCR3 and Autoimmune Diseases
 Necrosis Antibodies  >203 publications with CXCR3 and Necrosis
 Lung Diseases Antibodies  >183 publications with CXCR3 and Lung Diseases
 Hypersensitivity Antibodies  >139 publications with CXCR3 and Hypersensitivity
 Liver Diseases Antibodies  >120 publications with CXCR3 and Liver Diseases
 Carcinoma Antibodies  >107 publications with CXCR3 and Carcinoma
 Cardiovascular Diseases Antibodies  >95 publications with CXCR3 and Cardiovascular Diseases
 Fibrosis Antibodies  >78 publications with CXCR3 and Fibrosis
 Death Antibodies  >72 publications with CXCR3 and Death

Organs/Tissues associated with anti-CXCR3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1478 publications with CXCR3 and Blood
 Brain Antibodies  >139 publications with CXCR3 and Brain
 Spleen Antibodies  >119 publications with CXCR3 and Spleen
 Intestine Antibodies  >69 publications with CXCR3 and Intestine
 Muscle Antibodies  >55 publications with CXCR3 and Muscle
 Connective Tissue Antibodies  >15 publications with CXCR3 and Connective Tissue
 Uncharacterized Tissue Antibodies  >1 publications with CXCR3 and Uncharacterized Tissue
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