• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SLC1A3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247374

Slc1A3 is a member of a family of high-affinity sodium-dependent transporters that regulate the concentration of glutamate and aspartate in the excitatory synapses of the mammalian central nervous system. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLC1A3 / GLAST, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SLC1A3 / GLAST
Product Gene Name
anti-SLC1A3 antibody
Product Synonym Gene Name
GLAST
Product Synonym Names
Anti-SLC1A3 / GLAST Antibody (Cytoplasmic Domain) IHC-plus; SLC1A3; EA6; EAAT-1; EAAT1; GLAST; GLAST1; GLAST-1; Human SLC1A3; GLAST
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit
(Predicted: Dog, Guinea Pig, Chicken)
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
0.6mg/ml (lot specific)
Specificity
Human SLC1A3 / GLAST. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to one month. Avoid freeze/thaw cycles.
OMIM
gene 612656
3D Structure
ModBase 3D Structure for P43003
Target
SLC1A3 / EAAT1
Family/Subfamily
Transporter / Sodium:dicarboxylate symporter
Immunogen Species
SLC1A3 / EAAT1 was raised against Human
Antigen Type
Synthetic Peptide
Immunogen
SLC1A3 / EAAT1 antibody was raised against synthetic 19 amino acid peptide from cytoplasmic domain of human SLC1A3 / GLAST. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig (100%); Panda, Dog, Opossum, Guinea Pig, Turkey, Chicken (95%); Xenopus (84%).
Epitope
Cytoplasmic Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-SLC1A3 antibody

Family: Transporter; Subfamily: Sodium:dicarboxylate symporter
Applications Tested/Suitable for anti-SLC1A3 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-SLC1A3 antibody
IHC-P (5 ug/ml) - Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-SLC1A3 antibody

Anti-SLC1A3 / GLAST antibody IHC of human brain, cerebellum, molecular layer and Purkinje layer. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-SLC1A3 Polyclonal Antibody – Cat# MBS247374

Other Notes

Small volumes of anti-SLC1A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SLC1A3 purchase

MBS247374 is an antibody targeting SLC1A3. Also known as Rabbit Polyclonal to Human SLC1A3 / GLAST. Reactive in Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit
(Predicted: Dog, Guinea Pig, Chicken). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC1A3. It may not necessarily be applicable to this product.

NCBI GI #
169790839
NCBI GeneID
6507
NCBI Accession #
NP_004163.3
UniProt Primary Accession #
P43003
UniProt Secondary Accession #
Q4JCQ8; B2R5T3
UniProt Related Accession #
P43003
GenBank Nucleotide #
NM_004172.4
NCBI Official Full Name
excitatory amino acid transporter 1 isoform 1
NCBI Official Synonym Full Names
solute carrier family 1 (glial high affinity glutamate transporter), member 3
NCBI Official Symbol
SLC1A3
NCBI Official Synonym Symbols
EA6; EAAT1; GLAST; GLAST1
UniProt Protein Name
Excitatory amino acid transporter 1
UniProt Synonym Protein Names
Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3
Protein Family
Excitatory amino acid transporter
UniProt Gene Name
SLC1A3
UniProt Synonym Gene Names
EAAT1; GLAST; GLAST1; GLAST-1
UniProt Entry Name
EAA1_HUMAN
NCBI Protein Information
excitatory amino acid transporter 1; GLAST-1; solute carrier family 1 member 3; sodium-dependent glutamate/aspartate transporter 1

NCBI Summary for SLC1A3

This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
Show more

UniProt Comments for SLC1A3

SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Mitochondrial; Transporter

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cell projection; cell surface; cell soma; membrane; plasma membrane; fibril; integral to membrane

Molecular Function: glutamate binding; high-affinity glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity

Biological Process: response to drug; response to light stimulus; cranial nerve development; auditory behavior; glutamate biosynthetic process; positive regulation of synaptic transmission; response to antibiotic; L-glutamate import; synaptic transmission; sensory perception of sound; neuron morphogenesis during differentiation; gamma-aminobutyric acid biosynthetic process; response to wounding; neurotransmitter uptake; ion transport; neuromuscular process controlling balance; transmembrane transport

Disease: Episodic Ataxia, Type 6
Show more

Research Articles on SLC1A3

1. Its dysregulation may contribute to the pathology and possibly affect the onset of fragile X-associated tremor/ataxia syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SLC1A3 antibody

 Reference Product  PubMed Publications
 GFAP antibody  >41 publications with SLC1A3 and GFAP
 GLUL antibody  >5 publications with SLC1A3 and GLUL
 SLC2A1 antibody  >4 publications with SLC1A3 and SLC2A1
 GDNF antibody  >4 publications with SLC1A3 and GDNF
 SGK1 antibody  >1 publications with SLC1A3 and SGK1

Pathways associated with anti-SLC1A3 antibody

 Products by Pathway  Pathway Diagram
 Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway antibodies  Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Glutamatergic Synapse Pathway antibodies  Glutamatergic Synapse Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
 Neurotransmitter Uptake And Metabolism In Glial Cells Pathway antibodies  Neurotransmitter Uptake And Metabolism In Glial Cells Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Synaptic Vesicle Pathway antibodies  Synaptic Vesicle Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transmission Across Chemical Synapses Pathway antibodies  Transmission Across Chemical Synapses Pathway Diagram

Diseases associated with anti-SLC1A3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >108 publications with SLC1A3 and Nervous System Diseases
 Brain Diseases Antibodies  >77 publications with SLC1A3 and Brain Diseases
 Disease Models, Animal Antibodies  >54 publications with SLC1A3 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >32 publications with SLC1A3 and Cardiovascular Diseases
 Epilepsy Antibodies  >17 publications with SLC1A3 and Epilepsy
 Nerve Degeneration Antibodies  >13 publications with SLC1A3 and Nerve Degeneration
 Seizures Antibodies  >13 publications with SLC1A3 and Seizures
 Movement Disorders Antibodies  >11 publications with SLC1A3 and Movement Disorders
 Inflammation Antibodies  >10 publications with SLC1A3 and Inflammation
 Depressive Disorder Antibodies  >9 publications with SLC1A3 and Depressive Disorder

Organs/Tissues associated with anti-SLC1A3 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >240 publications with SLC1A3 and Brain
 Nerve Antibodies  >72 publications with SLC1A3 and Nerve
 Eye Antibodies  >47 publications with SLC1A3 and Eye
 Spinal Cord Antibodies  >30 publications with SLC1A3 and Spinal Cord
 Blood Antibodies  >30 publications with SLC1A3 and Blood
 Embryonic Tissue Antibodies  >19 publications with SLC1A3 and Embryonic Tissue
 Kidney Antibodies  >11 publications with SLC1A3 and Kidney
 Muscle Antibodies  >11 publications with SLC1A3 and Muscle
 Heart Antibodies  >7 publications with SLC1A3 and Heart
 Bone Antibodies  >6 publications with SLC1A3 and Bone
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000