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CAMK2A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247418

CAMK2Alpha, together with the beta, delta, and gamma subunits, constititute the CAMK2 heteromultimeric holoenzyme serine/threonine kinase. CAMK2A has been implicated in learning, where it potentiates synaptic transmission by phosphorylating AMPA receptors.… More details

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Product Overview

Online Datasheet in PDF
Product Name
CAMK2A / CaMKII Alpha, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human CAMK2A / CaMKII Alpha
Product Gene Name
anti-CAMK2A antibody
Product Synonym Names
Anti-CAMK2A / CaMKII Alpha Antibody (Internal) IHC-plus; CAMK2A; AlphaCaMKII; CaM-kinase II alpha chain; CaMK-II subunit alpha; CAMKA; CaM kinase II alpha; CaMK II alpha; CaMK-II alpha subunit; CaMKIINalpha; CaM kinase II alpha subunit; CaM kinase II subunit alpha; CaMKII alpha; CaMKIIalpha; KIAA0968; Human CAMK2A; CaMKII Alpha
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Bovine, Dog, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat
Form/Format
TBS, pH 7.3, 0.5% BSA, 0.02% Sodium Azide
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Human CAMK2A. This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1).
Preparation and Storage
Aliquot and store -20°C. Avoid freeze/thaw cycles.
OMIM
114078
3D Structure
ModBase 3D Structure for Q9UQM7
Target
CAMK2A/CaMKII Alpha
Epitope
aa455-468
Immunogen Species
Raised against human
Immunogen
CAMK2A / CaMKII Alpha antibody was raised against peptide with sequence C-PRTAQSEETRVWHR, from the internal region of the protein sequence according to NP_057065.2; NP_741960.1
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CAMK2A antibody

Family: Protein Kinase; Subfamily: CAMK2
Applications Tested/Suitable for anti-CAMK2A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CAMK2A antibody
ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Approx 55+65kDa bands observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2 and of 54.1 according to Human NP_741960.1 and Mouse NP_803126.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/ml.

Immunohistochemistry (IHC) of anti-CAMK2A antibody

Anti-CAMK2A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-CAMK2A Polyclonal Antibody – Cat# MBS247418

Immunohistochemistry (IHC) of anti-CAMK2A antibody

Anti-CAMK2A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-CAMK2A Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247418

Western Blot (WB) of anti-CAMK2A antibody

Antibody (0.1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-CAMK2A Polyclonal Antibody, Western Blot (WB) – Cat# MBS247418

Other Notes

Small volumes of anti-CAMK2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAMK2A purchase

MBS247418 is an antibody targeting CAMK2A. Also known as Goat Polyclonal to Human CAMK2A / CaMKII Alpha. Reactive in Bat, Bovine, Dog, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for CAMK2A. It may not necessarily be applicable to this product.

NCBI GI #
25952114
NCBI GeneID
815
NCBI Accession #
NP_057065.2
UniProt Primary Accession #
Q9UQM7
UniProt Secondary Accession #
Q9UL21; Q9Y2H4; Q9Y352
UniProt Related Accession #
Q9UQM7
GenBank Nucleotide #
NM_015981.3
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 1
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase II alpha
NCBI Official Symbol
CAMK2A
NCBI Official Synonym Symbols
CAMKA
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Protein Family
Calcium/calmodulin-dependent protein kinase
UniProt Gene Name
CAMK2A
UniProt Synonym Gene Names
CAMKA; KIAA0968; CaM kinase II subunit alpha; CaMK-II subunit alpha
UniProt Entry Name
KCC2A_HUMAN
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha

NCBI Summary for CAMK2A

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
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UniProt Comments for CAMK2A

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: presynaptic membrane; nucleoplasm; mitochondrion; plasma membrane; cell junction; nucleus; cytosol

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; protein binding; glutamate receptor binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; cytokine and chemokine mediated signaling pathway; calcium ion transport; protein amino acid autophosphorylation; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor
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Research Articles on CAMK2A

1. a novel regulation of CaMKII by another second messenger system and indicate its involvement in excitotoxic neuronal cell death.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAMK2A antibody

 Reference Product  PubMed Publications
 CAMK2B antibody  >9 publications with CAMK2A and CAMK2B
 CREB1 antibody  >1 publications with CAMK2A and CREB1
 GRIN2A antibody  >1 publications with CAMK2A and GRIN2A
 GRIN2B antibody  >1 publications with CAMK2A and GRIN2B

Pathways associated with anti-CAMK2A antibody

 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Adrenergic Signaling In Cardiomyocytes Pathway antibodies  Adrenergic Signaling In Cardiomyocytes Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 CREB Phosphorylation Through The Activation Of Ras Pathway antibodies  CREB Phosphorylation Through The Activation Of Ras Pathway Diagram
 Ca2+ Pathway antibodies  Ca2+ Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram

Diseases associated with anti-CAMK2A antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >48 publications with CAMK2A and Nervous System Diseases
 Brain Diseases Antibodies  >32 publications with CAMK2A and Brain Diseases
 Seizures Antibodies  >8 publications with CAMK2A and Seizures
 Pain Antibodies  >8 publications with CAMK2A and Pain
 Memory Disorders Antibodies  >6 publications with CAMK2A and Memory Disorders
 Movement Disorders Antibodies  >6 publications with CAMK2A and Movement Disorders
 Learning Disorders Antibodies  >5 publications with CAMK2A and Learning Disorders
 Inflammation Antibodies  >5 publications with CAMK2A and Inflammation
 Cognition Disorders Antibodies  >4 publications with CAMK2A and Cognition Disorders
 Necrosis Antibodies  >3 publications with CAMK2A and Necrosis

Organs/Tissues associated with anti-CAMK2A antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >140 publications with CAMK2A and Brain
 Muscle Antibodies  >6 publications with CAMK2A and Muscle
 Eye Antibodies  >4 publications with CAMK2A and Eye
 Embryonic Tissue Antibodies  >4 publications with CAMK2A and Embryonic Tissue
 Kidney Antibodies  >4 publications with CAMK2A and Kidney
 Heart Antibodies  >3 publications with CAMK2A and Heart
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