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PYGM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247446

MBS247446 is an antibody targeting PYGM. Also known as Rabbit Polyclonal to Human PYGM. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PYGM, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human PYGM
Product Gene Name
anti-PYGM antibody
Product Synonym Names
Anti-PYGM Antibody (C-Terminus) IHC-plus; PYGM; GPMM; Myophosphorylase; Phosphorylase; glycogen muscle; Human PYGM
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Ammonium sulfate precipitation
Preparation and Storage
Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.
Molecular Weight
87,317 Da
OMIM
phenotype 608455
3D Structure
ModBase 3D Structure for P11217
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide selected from the C-terminal region of human PYGM.
Immunogen Type
Synthetic peptide
Immunogen
PYGM antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human PYGM.
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-PYGM antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PYGM antibody
IHC-P (10 ug/ml), WB (1:1000)

Immunohistochemistry (IHC) of anti-PYGM antibody

Anti-PYGM / Phosphorylase b antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-PYGM Polyclonal Antibody – Cat# MBS247446

Immunohistochemistry (IHC) of anti-PYGM antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PYGM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PYGM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247446

Western Blot (WB) of anti-PYGM antibody

Western blot of PYGM (arrow) using rabbit polyclonal PYGM Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PYGM gene (Lane 2).
Rabbit Anti-PYGM Polyclonal Antibody, Western Blot (WB) – Cat# MBS247446

Other Notes

Small volumes of anti-PYGM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PYGM. It may not necessarily be applicable to this product.

NCBI GI #
5032009
NCBI GeneID
5837
NCBI Accession #
NP_005600.1
UniProt Primary Accession #
P11217
UniProt Secondary Accession #
A0AVK1; A6NDY6
UniProt Related Accession #
P11217
GenBank Nucleotide #
NM_005609.2
NCBI Official Full Name
glycogen phosphorylase, muscle form isoform 1
NCBI Official Synonym Full Names
phosphorylase, glycogen, muscle
NCBI Official Symbol
PYGM
UniProt Protein Name
Glycogen phosphorylase, muscle form
UniProt Synonym Protein Names
Myophosphorylase
Protein Family
Glycogen phosphorylase
UniProt Gene Name
PYGM
UniProt Entry Name
PYGM_HUMAN
NCBI Protein Information
glycogen phosphorylase, muscle form; myophosphorylase

NCBI Summary for PYGM

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]
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UniProt Comments for PYGM

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: EC 2.4.1.1; Transferase; Endoplasmic reticulum; Carbohydrate Metabolism - starch and sucrose; Phosphorylase

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytosol

Molecular Function: glycogen phosphorylase activity; nucleotide binding; pyridoxal phosphate binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis

Disease: Glycogen Storage Disease V
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Research Articles on PYGM

1. 5 different PYGM mutations were found in 8 Brazilian families: 4 previously described (p.R50X, p.T692kfs30, p.K609K, and p.G455R), and one, pI513V, a novel heterozygous mutation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PYGM antibody

 Reference Product  PubMed Publications
 FAU antibody  >4 publications with PYGM and FAU
 PHKB antibody  >3 publications with PYGM and PHKB
 GBE1 antibody  >2 publications with PYGM and GBE1
 PHKA1 antibody  >2 publications with PYGM and PHKA1
 PFKM antibody  >1 publications with PYGM and PFKM
 PHKG1 antibody  >1 publications with PYGM and PHKG1

Pathways associated with anti-PYGM antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycogen Metabolism Pathway antibodies  Glycogen Metabolism Pathway Diagram
 Glycogen Breakdown (glycogenolysis) Pathway antibodies  Glycogen Breakdown (glycogenolysis) Pathway Diagram
 Glycogen Storage Diseases Pathway antibodies  Glycogen Storage Diseases Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Insulin Signaling Pathway antibodies  Insulin Signaling Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Carbohydrates Pathway antibodies  Metabolism Of Carbohydrates Pathway Diagram

Diseases associated with anti-PYGM antibody

 Disease Name  Pubmed Publications
 Glycogen Storage Disease Type V Antibodies  >59 publications with PYGM and Glycogen Storage Disease Type V
 Nervous System Diseases Antibodies  >22 publications with PYGM and Nervous System Diseases
 Adenocarcinoma Antibodies  >15 publications with PYGM and Adenocarcinoma
 Hyperplasia Antibodies  >10 publications with PYGM and Hyperplasia
 Muscular Diseases Antibodies  >9 publications with PYGM and Muscular Diseases
 Lung Neoplasms Antibodies  >7 publications with PYGM and Lung Neoplasms
 Breast Neoplasms Antibodies  >6 publications with PYGM and Breast Neoplasms
 Skin Neoplasms Antibodies  >2 publications with PYGM and Skin Neoplasms
 Liver Diseases Antibodies  >2 publications with PYGM and Liver Diseases
 Hepatomegaly Antibodies  >2 publications with PYGM and Hepatomegaly

Organs/Tissues associated with anti-PYGM antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >87 publications with PYGM and Muscle
 Blood Antibodies  >17 publications with PYGM and Blood
 Pancreas Antibodies  >9 publications with PYGM and Pancreas
 Liver Antibodies  >8 publications with PYGM and Liver
 Lung Antibodies  >8 publications with PYGM and Lung
 Eye Antibodies  >4 publications with PYGM and Eye
 Brain Antibodies  >4 publications with PYGM and Brain
 Heart Antibodies  >4 publications with PYGM and Heart
 Nerve Antibodies  >3 publications with PYGM and Nerve
 Uterus Antibodies  >1 publications with PYGM and Uterus
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