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Alpha (FAP) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247487

Fibroblast Activation Protein Alpha (FAP) is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and… More details

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Product Overview

Online Datasheet in PDF
Product Name
FAP Alpha, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human FAP Alpha
Product Gene Name
anti-FAP antibody
Product Synonym Names
Anti-FAP Alpha Antibody (Extracellular Domain) IHC-plus; DPPIV; FAP alpha; Fibroblast activation protein; FAPA; Seprase; Human FAP Alpha
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Horse, Human, Monkey
Predicted Reactivity: Bovine, Pig
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human FAP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
OMIM
600403
3D Structure
ModBase 3D Structure for Q12884
Target
FAP Alpha
Immunogen
FAP Alpha antibody was raised against synthetic 17 amino acid peptide from extracellular domain of human FAP. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Horse (100%); Panda, Bovine, Pig (94%); Elephant, Dog (88%); Rat, Bat, Platypus (82%).
Immunogen Type
Synthetic peptide
Immunogen Species
FAP Alpha was raised against Human
Family/Subfamily
Protease / Serine S9
Epitope
Extracellular Domain
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-FAP antibody

Family: Protease; Subfamily: Serine S9
Applications Tested/Suitable for anti-FAP antibody
Immunohistochemistry-Paraffin (IHC-p)
Application Notes for anti-FAP antibody
IHC (5 ug/ml)
(Optimal dilution to be determined by the researcher.)

Immunohistochemistry (IHC) of anti-FAP antibody

Anti-FAP antibody IHC of human skin, hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-FAP Polyclonal Antibody – Cat# MBS247487

Other Notes

Small volumes of anti-FAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAP purchase

MBS247487 is an antibody targeting FAP. Also known as Rabbit Polyclonal to Human FAP Alpha. Reactive in Horse, Human, Monkey
Predicted Reactivity: Bovine, Pig. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FAP. It may not necessarily be applicable to this product.

NCBI GI #
16933540
NCBI GeneID
2191
NCBI Accession #
NP_004451.2
UniProt Primary Accession #
Q12884
UniProt Secondary Accession #
O00199; Q53TP5; Q86Z29; Q99998; Q9UID4
UniProt Related Accession #
Q12884
GenBank Nucleotide #
NM_004460.3
NCBI Official Full Name
prolyl endopeptidase FAP isoform 1
NCBI Official Synonym Full Names
fibroblast activation protein, alpha
NCBI Official Symbol
FAP
NCBI Official Synonym Symbols
FAPA; SIMP; DPPIV
UniProt Protein Name
Prolyl endopeptidase FAP
UniProt Synonym Protein Names
170 kDa melanoma membrane-bound gelatinase; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme; Surface-expressed protease;
Protein Family
Prolyl endopeptidase
UniProt Gene Name
FAP
UniProt Entry Name
SEPR_HUMAN
NCBI Protein Information
prolyl endopeptidase FAP; seprase; FAPalpha; dipeptidyl peptidase FAP; surface-expressed protease; post-proline cleaving enzyme; gelatine degradation protease FAP; integral membrane serine protease; serine integral membrane protease; 170 kDa melanoma membrane-bound gelatinase

NCBI Summary for FAP

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
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UniProt Comments for FAP

FAP: In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. Belongs to the peptidase S9B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.21.26; EC 3.4.14.5

Chromosomal Location of Human Ortholog: 2q23

Cellular Component: extracellular space; focal adhesion; cell surface; lamellipodium; apical part of cell; cytoplasm; integral to membrane; plasma membrane

Molecular Function: integrin binding; peptidase activity; protein dimerization activity; protein binding; protein homodimerization activity; protease binding; dipeptidyl-peptidase activity; serine-type peptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity; endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; endothelial cell migration; angiogenesis; proteolysis; cell adhesion; regulation of fibrinolysis
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Research Articles on FAP

1. A positive correlation between the expression of FAP-alpha and DPPIV and the activity of both gelatinases was found.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FAP antibody

 Reference Product  PubMed Publications
 DPP4 antibody  >17 publications with FAP and DPP4
 LOX antibody  >2 publications with FAP and LOX
 GIP antibody  >2 publications with FAP and GIP
 POSTN antibody  >1 publications with FAP and POSTN

Diseases associated with anti-FAP antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >601 publications with FAP and Carcinoma
 Adenocarcinoma Antibodies  >275 publications with FAP and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >161 publications with FAP and Cardiovascular Diseases
 Hypertrophy Antibodies  >102 publications with FAP and Hypertrophy
 Inflammation Antibodies  >91 publications with FAP and Inflammation
 Disease Models, Animal Antibodies  >89 publications with FAP and Disease Models, Animal
 Thyroid Diseases Antibodies  >70 publications with FAP and Thyroid Diseases
 Precancerous Conditions Antibodies  >46 publications with FAP and Precancerous Conditions
 Necrosis Antibodies  >44 publications with FAP and Necrosis
 Fibrosis Antibodies  >37 publications with FAP and Fibrosis

Organs/Tissues associated with anti-FAP antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >432 publications with FAP and Liver
 Intestine Antibodies  >414 publications with FAP and Intestine
 Vascular Antibodies  >175 publications with FAP and Vascular
 Muscle Antibodies  >172 publications with FAP and Muscle
 Stomach Antibodies  >143 publications with FAP and Stomach
 Eye Antibodies  >137 publications with FAP and Eye
 Brain Antibodies  >123 publications with FAP and Brain
 Thyroid Antibodies  >112 publications with FAP and Thyroid
 Skin Antibodies  >101 publications with FAP and Skin
 Lung Antibodies  >94 publications with FAP and Lung
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