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GRB2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247509

MBS247509 is an antibody targeting GRB2. Also known as Rabbit Polyclonal to Human GRB2. Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
GRB2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human GRB2
Product Gene Name
Product Synonym Names
Anti-GRB2 Antibody (C-Terminus) IHC-plus; GRB2; Abundant SRC homology; Adapter protein GRB2; ASH; EGFRBP-GRB2; MST084; MSTP084; Protein Ash; SH2/SH3 adapter GRB2; Grb3-3; HT027; NCKAP2; Human GRB2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
15 mM sodium azide, 0.2% BSA, PBS
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
The polyclonal antibody recognizes Grb2, a highly conserved approximately 26 kD adaptor protein. The immunogen has 100% identity between man, cow, rat and mouse.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
20,557 Da
OMIM
108355
3D Structure
ModBase 3D Structure for P62993
Target Species
Human
Immunogen Description
Synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).
Immunogen Type
Synthetic peptide
Immunogen
GRB2 antibody was raised against synthetic peptide corresponding to C-terminus of human Grb2 (amino acids 198-217).
Antigen Modification
C-Terminus
Applications Tested/Suitable for anti-GRB2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-GRB2 antibody
IHC-P (10 ug/ml), WB (1 ug/ml)
Usage: Western Blotting. Recommended dilution: 1 ug/ml. Positive control: RAMOS human Burkitt lymphoma cell line. Application note: Reducing conditions.

Immunohistochemistry (IHC) of anti-GRB2 antibody

Anti-GRB2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-GRB2 Polyclonal Antibody – Cat# MBS247509

Immunohistochemistry (IHC) of anti-GRB2 antibody

Anti-GRB2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-GRB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247509

Immunohistochemistry (IHC) of anti-GRB2 antibody

Anti-GRB2 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Rabbit Anti-GRB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247509

Other Notes

Small volumes of anti-GRB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
growth factor receptor-bound protein 2 isoform 1
NCBI Official Synonym Full Names
growth factor receptor-bound protein 2
NCBI Official Symbol
GRB2
NCBI Official Synonym Symbols
ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
UniProt Protein Name
Growth factor receptor-bound protein 2
UniProt Synonym Protein Names
Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2
UniProt Gene Name
GRB2
UniProt Synonym Gene Names
ASH
UniProt Entry Name
GRB2_HUMAN
NCBI Protein Information
growth factor receptor-bound protein 2; HT027; protein Ash; SH2/SH3 adapter GRB2; abundant SRC homology; growth factor receptor-bound protein 3; epidermal growth factor receptor-binding protein GRB2

NCBI Summary for GRB2

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for GRB2

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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