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NCKAP1L Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247522

MBS247522 is an antibody targeting NCKAP1L. Also known as Goat Polyclonal to Human NCKAP1L. Reactive in Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat. Host: Goat. More details

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Technical Datasheet View PDF
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Product Overview

Product Name
NCKAP1L, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human NCKAP1L
Product Gene Name
anti-NCKAP1L antibody
Product Synonym Names
Anti-NCKAP1L Antibody IHC-plus; NCKAP1L; HEM1; Hematopoietic protein 1; NCK-associated protein 1-like; Human NCKAP1L
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Bovine, Dog, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human NCKAP1L.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
122,478 Da
OMIM
141180
3D Structure
ModBase 3D Structure for P55160
Target Species
Human
Immunogen Description
Synthetic peptide from human NCKAP1L. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%); Elephant (92%); Xenopus (85%).
Immunogen
NCKAP1L antibody was raised against synthetic peptide from human NCKAP1L. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%); Elephant (92%); Xenopus (85%).
Applications Tested/Suitable for anti-NCKAP1L antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-NCKAP1L antibody
IHC-P (3.75 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-NCKAP1L antibody

Anti-NCKAP1L antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-NCKAP1L Polyclonal Antibody – Cat# MBS247522

Immunohistochemistry (IHC) of anti-NCKAP1L antibody

Anti-NCKAP1L antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Goat Anti-NCKAP1L Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247522

Other Notes

Small volumes of anti-NCKAP1L antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NCKAP1L. It may not necessarily be applicable to this product.

NCBI GI #
34485727
NCBI GeneID
3071
NCBI Accession #
NP_005328.2
UniProt Primary Accession #
P55160
UniProt Secondary Accession #
Q52LW0; B4DUT5
UniProt Related Accession #
P55160
GenBank Nucleotide #
NM_005337.4
NCBI Official Full Name
nck-associated protein 1-like isoform 1
NCBI Official Synonym Full Names
NCK-associated protein 1-like
NCBI Official Symbol
NCKAP1L
NCBI Official Synonym Symbols
HEM1
UniProt Protein Name
Nck-associated protein 1-like
UniProt Synonym Protein Names
Hematopoietic protein 1; Membrane-associated protein HEM-1
Protein Family
Nck-associated protein
UniProt Gene Name
NCKAP1L
UniProt Synonym Gene Names
HEM1
UniProt Entry Name
NCKPL_HUMAN
NCBI Protein Information
nck-associated protein 1-like; hematopoietic protein 1; membrane-associated protein hem-1

NCBI Summary for NCKAP1L

This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]
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UniProt Comments for NCKAP1L

HEM1: a component of the Scar/WAVE complex that plays an important role in regulating cell shape and motility. Found at elevated levels in cells of hematopoietic lineage. Localizes to propagating waves at the leading edge of a motile human neutrophils. The complex stimulates actin assembly; polymerized actin is reciprocally required to remove the complex from the membrane. Overexpression is associated with poor outcome in B-cell chronic lymphocytic leukemia patients.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: membrane; integral to plasma membrane; cytosol

Molecular Function: protein binding; protein complex binding; protein kinase activator activity

Biological Process: response to drug; neutrophil chemotaxis; erythrocyte development; positive regulation of erythrocyte differentiation; myeloid cell homeostasis; positive regulation of cell adhesion mediated by integrin; positive regulation of CD4-positive, alpha beta T cell differentiation; maintenance of cell polarity; chemotaxis; positive regulation of CD8-positive, alpha-beta T cell differentiation; positive regulation of lymphocyte differentiation; B cell receptor signaling pathway; negative regulation of interleukin-17 production; B cell homeostasis; positive regulation of actin filament polymerization; positive regulation of protein kinase activity; negative regulation of interleukin-6 production; positive regulation of B cell proliferation; positive regulation of B cell differentiation; positive regulation of T cell proliferation; T cell homeostasis; protein complex assembly; positive regulation of gamma-delta T cell differentiation; cortical actin cytoskeleton organization and biogenesis; positive regulation of phagocytosis, engulfment; positive regulation of phosphorylation; negative regulation of apoptosis
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Research Articles on NCKAP1L

1. the Hem-1/Nap1 component of the Scar/WAVE complex localizes to propagating waves that appear to organize the leading edge of a motile neutrophil

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCKAP1L antibody

 Reference Product  PubMed Publications
 LAPTM5 antibody  >1 publications with NCKAP1L and LAPTM5
 PTPRC antibody  >1 publications with NCKAP1L and PTPRC
 WASF1 antibody  >1 publications with NCKAP1L and WASF1
 CD53 antibody  >1 publications with NCKAP1L and CD53

Pathways associated with anti-NCKAP1L antibody

 Products by Pathway  Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram

Diseases associated with anti-NCKAP1L antibody

 Disease Name  Pubmed Publications
 Ovarian Diseases Antibodies  >1 publications with NCKAP1L and Ovarian Diseases
 Lung Diseases Antibodies  >1 publications with NCKAP1L and Lung Diseases
 Immune System Diseases Antibodies  >1 publications with NCKAP1L and Immune System Diseases
 Kidney Diseases Antibodies  >1 publications with NCKAP1L and Kidney Diseases

Organs/Tissues associated with anti-NCKAP1L antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >5 publications with NCKAP1L and Blood
 Liver Antibodies  >1 publications with NCKAP1L and Liver
 Testis Antibodies  >1 publications with NCKAP1L and Testis
 Lung Antibodies  >1 publications with NCKAP1L and Lung
 Kidney Antibodies  >1 publications with NCKAP1L and Kidney
 Brain Antibodies  >1 publications with NCKAP1L and Brain
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