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IARS2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247618

MBS247618 is an antibody targeting IARS2. Also known as Rabbit Polyclonal (IgG) to Human IARS2. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
IARS2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human IARS2
Product Gene Name
Product Synonym Names
Anti-IARS2 Antibody (aa71-120) IHC-plus; IARS2; Isoleucyl-tRNA synthetase; ILERS; Human IARS2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
IARS2 Antibody detects endogenous levels of total IARS2 protein.
Preparation and Storage
Store at -20 degree C for up to one year.
Molecular Weight
113,792 Da
OMIM
612801
3D Structure
ModBase 3D Structure for Q9NSE4
Target Species
Human
Immunogen Description
Synthetic peptide from human IARS2 (aa71-120).
Immunogen
IARS2 antibody was raised against synthetic peptide from human IARS2 (aa71-120).
Antigen Modification
aa71-120
Applications Tested/Suitable for anti-IARS2 antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-IARS2 antibody
ELISA (1:1000), IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-IARS2 antibody

Anti-IARS2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
Rabbit Anti-IARS2 Polyclonal Antibody – Cat# MBS247618

Immunofluorescence (IF) of anti-IARS2 antibody

Immunofluorescence of HepG2 cells, using IARS2 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-IARS2 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS247618

Western Blot (WB) of anti-IARS2 antibody

Western blot of extracts from RAW264.7/COS7 cells, using IARS2 Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-IARS2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247618

Other Notes

Small volumes of anti-IARS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for IARS2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
isoleucine--tRNA ligase, mitochondrial
NCBI Official Synonym Full Names
isoleucyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol
IARS2
NCBI Official Synonym Symbols
ILERS; CAGSSS
UniProt Protein Name
Isoleucine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names
Isoleucyl-tRNA synthetase; IleRS
UniProt Gene Name
IARS2
UniProt Synonym Gene Names
IleRS
UniProt Entry Name
SYIM_HUMAN
NCBI Protein Information
isoleucine--tRNA ligase, mitochondrial; isoleucine tRNA ligase 2, mitochondrial; isoleucyl-tRNA synthetase, mitochondrial; mitochondrial isoleucine tRNA synthetase; isoleucine-tRNA synthetase 2, mitochondrial

NCBI Summary for IARS2

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]
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UniProt Comments for IARS2

IARS2: Belongs to the class-I aminoacyl-tRNA synthetase family.

Protein type: Mitochondrial; Ligase; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Translation; EC 6.1.1.5

Chromosomal Location of Human Ortholog: 1q41

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; cytosol

Molecular Function: ATP binding; isoleucine-tRNA ligase activity

Biological Process: isoleucyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; mitochondrial translation; regulation of translational fidelity; gene expression

Disease: Cataracts, Growth Hormone Deficiency, Sensory Neuropathy, Sensorineural Hearing Loss, And Skeletal Dysplasia
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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