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Prohibitin 2 / PHB2 (PHB2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247641

MBS247641 is an antibody targeting PHB2. Also known as Rabbit Polyclonal to Human Prohibitin 2 / PHB2. Reactive in Human, Mouse. Host: Rabbit. More details

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Testing Data View PDF
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Product Overview

Product Name
Prohibitin 2 / PHB2, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human Prohibitin 2 / PHB2
Product Gene Name
anti-PHB2 antibody
Product Synonym Names
Anti-Prohibitin 2 / PHB2 Antibody (Tyr128) IHC-plus; PHB2; BCAP37; BAP; D-prohibitin; p22; Prohibitin-2; B-cell associated protein; Bap37; PNAS-141; Prohibitin 2; REA; Human Prohibitin 2; PHB2
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity Purified
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
Molecular Weight
29,044 Da
OMIM
610704
3D Structure
ModBase 3D Structure for Q99623
Target Species
Human
Immunogen Description
KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y128 of human PHB2.
Immunogen Type
Synthetic peptide
Immunogen
Prohibitin 2 / PHB2 antibody was raised against kLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y128 of human PHB2.
Antigen Modification
Tyr128

Product Categories/Family for anti-PHB2 antibody

Family: Transcription factor
Applications Tested/Suitable for anti-PHB2 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-PHB2 antibody
IHC-P (5 ug/ml), WB (1:1000)
Usage: Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time. Mouse is a predicted species only.

Immunohistochemistry (IHC) of anti-PHB2 antibody

Anti-PHB2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PHB2 Polyclonal Antibody – Cat# MBS247641

Immunohistochemistry (IHC) of anti-PHB2 antibody

Anti-PHB2 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-PHB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247641

Immunohistochemistry (IHC) of anti-PHB2 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (Y128), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-PHB2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247641

Western Blot (WB) of anti-PHB2 antibody

Western blot of PHB2-Y128 in mouse NIH-3T3 cell line lysates (35 ug/lane). PHB2 (arrow) was detected using the purified Pab.
Rabbit Anti-PHB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247641

Western Blot (WB) of anti-PHB2 antibody

Western blot of PHB2-Y128 in 293 cell line lysates (35 ug/lane). PHB2 (arrow) was detected using the purified Pab.
Rabbit Anti-PHB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247641

Western Blot (WB) of anti-PHB2 antibody

Western blot of PHB2 (arrow) using rabbit polyclonal PHB2 Antibody (Y128). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2).
Rabbit Anti-PHB2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247641

Other Notes

Small volumes of anti-PHB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PHB2. It may not necessarily be applicable to this product.

NCBI GI #
221307584
NCBI GeneID
11331
NCBI Accession #
NP_001138303.1
UniProt Primary Accession #
Q99623
UniProt Secondary Accession #
Q2YDA4; Q7KYU3; Q92978; B4DP75
UniProt Related Accession #
Q99623
GenBank Nucleotide #
NM_001144831.1
NCBI Official Full Name
prohibitin-2 isoform 1
NCBI Official Synonym Full Names
prohibitin 2
NCBI Official Symbol
PHB2
NCBI Official Synonym Symbols
BAP; REA; p22; Bap37; BCAP37; PNAS-141
UniProt Protein Name
Prohibitin-2
UniProt Synonym Protein Names
B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity
Protein Family
Prohibitin
UniProt Gene Name
PHB2
UniProt Entry Name
PHB2_HUMAN
NCBI Protein Information
prohibitin-2; PHB2; D-prohibitin; B-cell associated protein; repressor of estrogen receptor activity; B-cell receptor-associated protein BAP37

UniProt Comments for PHB2

BAP37: a protein of the band 7 family. This family includes the prohibitins, cytoplasmic anti-proliferative proteins and stomatin, an erythrocyte membrane protein. A repressor of estrogen receptor (ER) activity. Reverses steroid receptor coactivator 1 enhancement of ER activity. May play a role in determining the sensitivity of estrogen target cells to antiestrogen and estrogen therapy.

Protein type: Nuclear receptor co-regulator; Mitochondrial; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: nuclear matrix; protein complex; mitochondrion; mitochondrial inner membrane; nucleus

Molecular Function: protein binding; estrogen receptor binding

Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent; negative regulation of mammary gland epithelial cell proliferation; negative regulation of estrogen receptor signaling pathway
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Research Articles on PHB2

1. PHB2 in hepatocellular carcinoma supports the development and progression of hepatocellular malignancy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PHB2 antibody

 Reference Product  PubMed Publications
 PHB antibody  >22 publications with PHB2 and PHB
 ESR1 antibody  >3 publications with PHB2 and ESR1
 AKT1 antibody  >1 publications with PHB2 and AKT1

Diseases associated with anti-PHB2 antibody

 Disease Name  Pubmed Publications
 Weight Loss Antibodies  >4 publications with PHB2 and Weight Loss
 Liver Diseases Antibodies  >3 publications with PHB2 and Liver Diseases
 Breast Neoplasms Antibodies  >3 publications with PHB2 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with PHB2 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >2 publications with PHB2 and Carcinoma, Hepatocellular
 Atrophy Antibodies  >2 publications with PHB2 and Atrophy
 Liver Neoplasms Antibodies  >2 publications with PHB2 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >1 publications with PHB2 and Neoplasms, Experimental
 Precancerous Conditions Antibodies  >1 publications with PHB2 and Precancerous Conditions
 Hypertrophy Antibodies  >1 publications with PHB2 and Hypertrophy

Organs/Tissues associated with anti-PHB2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with PHB2 and Blood
 Muscle Antibodies  >7 publications with PHB2 and Muscle
 Liver Antibodies  >4 publications with PHB2 and Liver
 Heart Antibodies  >4 publications with PHB2 and Heart
 Brain Antibodies  >2 publications with PHB2 and Brain
 Prostate Antibodies  >1 publications with PHB2 and Prostate
 Ovary Antibodies  >1 publications with PHB2 and Ovary
 Uterus Antibodies  >1 publications with PHB2 and Uterus
 Intestine Antibodies  >1 publications with PHB2 and Intestine
 Uncharacterized Tissue Antibodies  >1 publications with PHB2 and Uncharacterized Tissue
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