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USP10 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247674

MBS247674 is an antibody targeting USP10. Also known as Rabbit Polyclonal to Human USP10. Reactive in Human, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
USP10, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human USP10
Product Gene Name
Product Synonym Names
Anti-USP10 Antibody (N-Terminus) IHC-plus; USP10; KIAA0190; Ubiquitin thiolesterase 10; UBPO; Ubiquitin specific protease 10; Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Human USP10
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
PBS, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human USP10
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Molecular Weight
87,533 Da
OMIM
609818
3D Structure
ModBase 3D Structure for Q14694
Target Species
Human
Immunogen Description
A 16 amino acid peptide near the amino terminus of human USP10.
Immunogen Type
Synthetic peptide
Immunogen
USP10 antibody was raised against a 16 amino acid peptide near the amino terminus of human USP10.
Antigen Modification
N-Terminus
Applications Tested/Suitable for anti-USP10 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
Application Notes for anti-USP10 antibody
ELISA, ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: USP10 antibody can be used for detection of USP10 by Western blot at 1-2 ug/ml.

Immunohistochemistry (IHC) of anti-USP10 antibody

Anti-USP10 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody – Cat# MBS247674

Immunohistochemistry (IHC) of anti-USP10 antibody

Anti-USP10 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247674

Immunohistochemistry (IHC) of anti-USP10 antibody

Anti-USP10 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247674

Immunofluorescence (IF) of anti-USP10 antibody

Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS247674

Immunocytochemistry (ICC) of anti-USP10 antibody

Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS247674

Western Blot (WB) of anti-USP10 antibody

Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 ug/ml.
Rabbit Anti-USP10 Polyclonal Antibody, Western Blot (WB) – Cat# MBS247674

Other Notes

Small volumes of anti-USP10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for USP10. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 10 isoform 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 10
NCBI Official Symbol
USP10
NCBI Official Synonym Symbols
UBPO
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 10
UniProt Synonym Protein Names
Deubiquitinating enzyme 10; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10
UniProt Gene Name
USP10
UniProt Synonym Gene Names
KIAA0190
UniProt Entry Name
UBP10_HUMAN
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 10; ubiquitin thioesterase 10; deubiquitinating enzyme 10; ubiquitin thiolesterase 10; ubiquitin specific protease 10; ubiquitin-specific-processing protease 10

NCBI Summary for USP10

Ubiquitin is a highly conserved protein that is covalently linked to other proteins to regulate their function and degradation. This gene encodes a member of the ubiquitin-specific protease family of cysteine proteases. The enzyme specifically cleaves ubiquitin from ubiquitin-conjugated protein substrates. The protein is found in the nucleus and cytoplasm. It functions as a co-factor of the DNA-bound androgen receptor complex, and is inhibited by a protein in the Ras-GTPase pathway. The human genome contains several pseudogenes similar to this gene. Several transcript variants, some protein-coding and others not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2013]
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UniProt Comments for USP10

USP10: a carboxyl-terminal ubiquitin-processing protease. Ras-GAP SH3 domain-binding protein (G3BP) appears to inhibit its ability to disassemble ubiquitin chains.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; RNA-binding; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 16q24.1

Cellular Component: early endosome; cytoplasm; nucleus

Molecular Function: protein binding; p53 binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; DNA damage response, signal transduction by p53 class mediator; autophagy; regulation of autophagy; DNA repair
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Research Articles on USP10

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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