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SYVN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247950

MBS247950 is an antibody targeting SYVN1. Also known as Rabbit Polyclonal to Human SYVN1 / HRD1. Reactive in Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat, Zebrafish. Host: Rabbit. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SYVN1 / HRD1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SYVN1 / HRD1
Product Gene Name
anti-SYVN1 antibody
Product Synonym Gene Name
HRD1
Product Synonym Names
Anti-SYVN1 / HRD1 Antibody (Internal) IHC-plus; SYVN1; KIAA1810; Synoviolin; Synovial apoptosis inhibitor 1; DER3; HRD1; Human SYVN1; HRD1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat, Zebrafish
Form/Format
PBS, 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human SYVN1 / HRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to one month. Avoid freeze-thaw cycles.
OMIM
608046
3D Structure
ModBase 3D Structure for Q86TM6
Target Species
Human
Immunogen Description
Synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1.
Antigen Type
Synthetic peptide
Immunogen
SYVN1 / HRD1 antibody was raised against synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (100%).
Antigen Modification
Internal
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-SYVN1 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-SYVN1 antibody
IHC-P (5 - 10 ug/ml)

Immunohistochemistry (IHC) of anti-SYVN1 antibody

Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-SYVN1 Polyclonal Antibody – Cat# MBS247950

Other Notes

Small volumes of anti-SYVN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYVN1. It may not necessarily be applicable to this product.

NCBI GI #
27436925
NCBI GeneID
84447
NCBI Accession #
NP_115807.1
UniProt Primary Accession #
Q86TM6
UniProt Secondary Accession #
Q8N3K3; Q8N6E8; Q96JL5; Q96PK3
UniProt Related Accession #
Q86TM6
GenBank Nucleotide #
NM_032431.2
NCBI Official Full Name
E3 ubiquitin-protein ligase synoviolin isoform a
NCBI Official Synonym Full Names
synovial apoptosis inhibitor 1, synoviolin
NCBI Official Symbol
SYVN1
NCBI Official Synonym Symbols
DER3; HRD1
UniProt Protein Name
E3 ubiquitin-protein ligase synoviolin
UniProt Synonym Protein Names
Synovial apoptosis inhibitor 1
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
SYVN1
UniProt Synonym Gene Names
HRD1; KIAA1810
UniProt Entry Name
SYVN1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog

NCBI Summary for SYVN1

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]
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UniProt Comments for SYVN1

SYVN1: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Belongs to the HRD1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Endoplasmic reticulum; Membrane protein, multi-pass; Ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding

Biological Process: ER-associated protein catabolic process; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; in utero embryonic development; unfolded protein response; protein ubiquitination; protein amino acid N-linked glycosylation via asparagine
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Research Articles on SYVN1

1. Herp localizes to the endoplasmic reticulum-derived quality control compartment (ERQC) and recruits HRD1, which targets to endoplasmic reticulum associated degradation the substrate presented by the OS-9 lectin at the ERQC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SYVN1 antibody

 Reference Product  PubMed Publications
 SEL1L antibody  >19 publications with SYVN1 and SEL1L
 VCP antibody  >7 publications with SYVN1 and VCP
 DERL1 antibody  >4 publications with SYVN1 and DERL1
 UBE2G2 antibody  >4 publications with SYVN1 and UBE2G2
 DERL2 antibody  >2 publications with SYVN1 and DERL2
 NPLOC4 antibody  >2 publications with SYVN1 and NPLOC4
 HSPA5 antibody  >1 publications with SYVN1 and HSPA5
 ERN1 antibody  >1 publications with SYVN1 and ERN1

Pathways associated with anti-SYVN1 antibody

 Products by Pathway  Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 HRD1/SEL1 ERAD Complex Pathway antibodies  HRD1/SEL1 ERAD Complex Pathway Diagram
 HRD1/SEL1 ERAD Complex Pathway antibodies  HRD1/SEL1 ERAD Complex Pathway Diagram
 Hedgehog Ligand Biogenesis Pathway antibodies  Hedgehog Ligand Biogenesis Pathway Diagram
 Hh Ligand Biogenesis Disease Pathway antibodies  Hh Ligand Biogenesis Disease Pathway Diagram
 IRE1alpha Activates Chaperones Pathway antibodies  IRE1alpha Activates Chaperones Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Processing-defective Hh Variants Abrogate Ligand Secretion Pathway antibodies  Processing-defective Hh Variants Abrogate Ligand Secretion Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram
 Protein Processing In Endoplasmic Reticulum Pathway antibodies  Protein Processing In Endoplasmic Reticulum Pathway Diagram

Diseases associated with anti-SYVN1 antibody

 Disease Name  Pubmed Publications
 Autoimmune Diseases Antibodies  >10 publications with SYVN1 and Autoimmune Diseases
 Fibrosis Antibodies  >3 publications with SYVN1 and Fibrosis
 Inflammation Antibodies  >3 publications with SYVN1 and Inflammation
 Necrosis Antibodies  >3 publications with SYVN1 and Necrosis
 Liver Diseases Antibodies  >2 publications with SYVN1 and Liver Diseases
 Disease Models, Animal Antibodies  >2 publications with SYVN1 and Disease Models, Animal
 Hyperplasia Antibodies  >2 publications with SYVN1 and Hyperplasia
 Fetal Death Antibodies  >1 publications with SYVN1 and Fetal Death
 Seizures Antibodies  >1 publications with SYVN1 and Seizures
 Kidney Diseases Antibodies  >1 publications with SYVN1 and Kidney Diseases

Organs/Tissues associated with anti-SYVN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >10 publications with SYVN1 and Brain
 Nerve Antibodies  >4 publications with SYVN1 and Nerve
 Blood Antibodies  >4 publications with SYVN1 and Blood
 Skin Antibodies  >4 publications with SYVN1 and Skin
 Liver Antibodies  >3 publications with SYVN1 and Liver
 Bone Antibodies  >2 publications with SYVN1 and Bone
 Kidney Antibodies  >1 publications with SYVN1 and Kidney
 Lung Antibodies  >1 publications with SYVN1 and Lung
 Intestine Antibodies  >1 publications with SYVN1 and Intestine
 Pancreas Antibodies  >1 publications with SYVN1 and Pancreas
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