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TACR3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247956

NK3R is a Tachykinin Receptor specific for neurokinin B, although its function is not well understood. More details

$630 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TACR3 / NK3R, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human TACR3 / NK3R
Product Gene Name
anti-TACR3 antibody
Product Synonym Gene Name
NK3R
Product Synonym Names
Anti-TACR3 / NK3R Antibody (C-Terminus) IHC-plus; TACR3; HH11; Nmk-r; NK3R; NKR; Tachykinin receptor 3; Neurokinin B; Neurokinin B receptor; NK-3 receptor; NK-3R; TAC3R; Neurokinin 3 receptor; Neurokinin beta receptor; Neuromedin-K receptor; TAC3RL; Human TACR3; NK3R
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human; Predicted: Monkey
Form/Format
PBS, less than 0.1% sodium azide.
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
Human TACR3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Preparation and Storage
Upon arrival: aliquot and store undiluted at -20°C or below for up to 1 year.
Can be stored undiluted at 4°C for up to 1 month.
Avoid freeze/thaw cycles
Molecular Weight
52,202 Da
OMIM
gene 614840
3D Structure
ModBase 3D Structure for P29371
Target
TACR3/NK3R
Immunogen
Antibody was raised against Synthetic 17 amino acid peptide from C-terminal cytoplasmic domain of human TACR3. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey (100%); Gibbon, Marmoset (94%); Mouse, Rat, Hamster, Panda, Bovine, Dog, Rabbit (88%); Elephant, Bat (82%).
Immunogen Species
TACR3 / NK3R antibody was raised against Human
Epitope
C-Terminus

Product Categories/Family for anti-TACR3 antibody

Family: GPCR; Subfamily: Tachykinin
Applications Tested/Suitable for anti-TACR3 antibody
Immunohistochemistry (IHC - Paraffin)
Application Notes for anti-TACR3 antibody
IHC-P (20 ug/ml)
(Optimal dilution to be determined by the researcher)

Disclaimer||Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.!! !!Recommended Immunohistochemistry Protocol|| The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Immunohistochemistry (IHC) of anti-TACR3 antibody

Anti-TACR3 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-TACR3 Polyclonal Antibody – Cat# MBS247956

Other Notes

Small volumes of anti-TACR3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TACR3 purchase

MBS247956 is an antibody targeting TACR3. Also known as Rabbit Polyclonal to Human TACR3 / NK3R. Reactive in Human; Predicted: Monkey. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TACR3. It may not necessarily be applicable to this product.

NCBI GI #
7669548
NCBI GeneID
6870
NCBI Accession #
NP_001050.1
UniProt Primary Accession #
P29371
UniProt Secondary Accession #
Q0P510
UniProt Related Accession #
P29371
GenBank Nucleotide #
NM_001059.2
NCBI Official Full Name
neuromedin-K receptor
NCBI Official Synonym Full Names
tachykinin receptor 3
NCBI Official Symbol
TACR3
NCBI Official Synonym Symbols
NKR; HH11; NK3R; NK-3R; TAC3RL
UniProt Protein Name
Neuromedin-K receptor
UniProt Synonym Protein Names
NK-3 receptor; NK-3R; Neurokinin B receptor; Tachykinin receptor 3
Protein Family
Neuromedin-K receptor
UniProt Gene Name
TACR3
UniProt Synonym Gene Names
NK3R; TAC3R; NKR; NK-3R
UniProt Entry Name
NK3R_HUMAN
NCBI Protein Information
neuromedin-K receptor; NK-3 receptor; neurokinin B receptor; neurokinin beta receptor

NCBI Summary for TACR3

This gene belongs to a family of genes that function as receptors for tachykinins. Receptor affinities are specified by variations in the 5'-end of the sequence. The receptors belonging to this family are characterized by interactions with G proteins and 7 hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin neurokinin 3, also referred to as neurokinin B. [provided by RefSeq, Jul 2008]
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UniProt Comments for TACR3

TACR3: This is a receptor for the tachykinin neuropeptide neuromedin-K (neurokinin B). It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: neuromedin-K > substance K > substance P. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; tachykinin receptor activity

Biological Process: tachykinin signaling pathway; hyperosmotic salinity response; regulation of dopamine metabolic process; response to morphine; positive regulation of heart rate; response to cocaine; response to estradiol stimulus; positive regulation of blood pressure; aging

Disease: Hypogonadotropic Hypogonadism 11 With Or Without Anosmia
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Research Articles on TACR3

1. data indicate that GPR54 and TACR3 gene mutations are not a frequent cause of ICPP. The identified A/G synonymous SNP (dbSNP ID: rs10407968) located in exon 1 of the gene is not likely to have a pathogenic role in exon splicing

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TACR3 antibody

 Reference Product  PubMed Publications
 TAC3 antibody  >39 publications with TACR3 and TAC3
 KISS1R antibody  >20 publications with TACR3 and KISS1R
 KISS1 antibody  >16 publications with TACR3 and KISS1
 PROK2 antibody  >15 publications with TACR3 and PROK2
 GNRH1 antibody  >11 publications with TACR3 and GNRH1
 TAC1 antibody  >6 publications with TACR3 and TAC1

Pathways associated with anti-TACR3 antibody

 Products by Pathway  Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Metabolic Disorders Of Biological Oxidation Enzymes Pathway antibodies  Metabolic Disorders Of Biological Oxidation Enzymes Pathway Diagram

Diseases associated with anti-TACR3 antibody

 Disease Name  Pubmed Publications
 Hypogonadism Antibodies  >35 publications with TACR3 and Hypogonadism
 Idiopathic Hypogonadotropic Hypogonadism Antibodies  >10 publications with TACR3 and Idiopathic Hypogonadotropic Hypogonadism
 Disorders of Sex Development Antibodies  >9 publications with TACR3 and Disorders of Sex Development
 Hypertension Antibodies  >2 publications with TACR3 and Hypertension
 Cocaine-Related Disorders Antibodies  >2 publications with TACR3 and Cocaine-Related Disorders
 Brain Diseases Antibodies  >1 publications with TACR3 and Brain Diseases
 Genital Diseases, Male Antibodies  >1 publications with TACR3 and Genital Diseases, Male
 Memory Disorders Antibodies  >1 publications with TACR3 and Memory Disorders
 Learning Disorders Antibodies  >1 publications with TACR3 and Learning Disorders
 Cognition Disorders Antibodies  >1 publications with TACR3 and Cognition Disorders

Organs/Tissues associated with anti-TACR3 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with TACR3 and Muscle
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