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CKM Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS247970

MBS247970 is an antibody targeting CKM. Also known as Rabbit Polyclonal (IgG) to Human CKM / Creatine Kinase MM. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
CKM / Creatine Kinase MM, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human CKM / Creatine Kinase MM
Product Gene Name
Product Synonym Names
Anti-CKM / Creatine Kinase MM Antibody (aa10-59) IHC-plus; CKM; CK-MB; CKMM; Creatine kinase M-type; Creatine kinase; muscle; M-CK; Creatine kinase M chain; Human CKM; Creatine Kinase MM
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/ml (lot specific)
Specificity
M-CK Antibody detects endogenous levels of total M-CK protein.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
43,101 Da
OMIM
123310
3D Structure
ModBase 3D Structure for P06732
Target Species
Human
Immunogen Description
Synthetic peptide from human M-CK (aa10-59).
Immunogen
CKM / Creatine Kinase MM antibody was raised against synthetic peptide from human M-CK (aa10-59).
Antigen Modification
aa10-59
Applications Tested/Suitable for anti-CKM antibody
Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Application Notes for anti-CKM antibody
ELISA (1:40000), IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:500 - 1:1000)

Immunohistochemistry (IHC) of anti-CKM antibody

Anti-CKM antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-CKM Polyclonal Antibody – Cat# MBS247970

Immunohistochemistry (IHC) of anti-CKM antibody

Anti-CKM antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-CKM Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS247970

Immunofluorescence (IF) of anti-CKM antibody

Immunofluorescence of HepG2 cells, using CKM Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-CKM Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS247970

Western Blot (WB) of anti-CKM antibody

Western blot of extracts from Jurkat/HeLa/HepG2/HUVEC cells, using CKM Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit Anti-CKM Polyclonal Antibody, Western Blot (WB) – Cat# MBS247970

Other Notes

Small volumes of anti-CKM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CKM. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
creatine kinase M-type
NCBI Official Synonym Full Names
creatine kinase, muscle
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
CKMM; M-CK
UniProt Protein Name
Creatine kinase M-type
UniProt Synonym Protein Names
Creatine kinase M chain; M-CKCreatine kinase M-type, N-terminally processed
Protein Family
UniProt Gene Name
CKM
UniProt Synonym Gene Names
CKMM
UniProt Entry Name
KCRM_HUMAN
NCBI Protein Information
creatine kinase M-type; creatine kinase M chain

NCBI Summary for CKM

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
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UniProt Comments for CKM

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: EC 2.7.3.2; Amino Acid Metabolism - arginine and proline; Kinase, other

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphocreatine biosynthetic process; creatine metabolic process; phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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