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JHDM1A Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248036

MBS248036 is an antibody targeting JHDM1A. Also known as Goat Polyclonal to Human JHDM1A / KDM2A. Reactive in Bat, Gibbon, Dog, Hamster, Horse, Human, Monkey, Pig, Rabbit, RatPredicted Reactivity: Mouse (at least 90% immunogen sequence identity). Host: Goat. More details

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Product Overview

Online Datasheet in PDF
Product Name
JHDM1A / KDM2A, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human JHDM1A / KDM2A
Product Gene Name
anti-JHDM1A antibody
Product Synonym Gene Name
KDM2A
Product Synonym Names
Anti-JHDM1A / KDM2A Antibody (Internal) IHC-plus; KDM2A; CXXC8; FBL11; DKFZP434M1735; JHDM1A; F-box protein Fbl7; F-box/LRR-repeat protein 11; KIAA1004; FBXL11; Lysine-specific demethylase 2A; LILINA; SF-KDM2A; F-box protein Lilina; FBL7; Human JHDM1A; KDM2A
Clonality
Polyclonal
Host
Goat
Species Reactivity
Bat, Gibbon, Dog, Hamster, Horse, Human, Monkey, Pig, Rabbit, Rat
Predicted Reactivity: Mouse (at least 90% immunogen sequence identity)
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human KDM2A / FBXL11.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.
Molecular Weight
89,948 Da
OMIM
605657
3D Structure
ModBase 3D Structure for Q9Y2K7
Target Species
Human
Immunogen Description
Synthetic peptide C-PRKDRQVHLTHFELE from an internal region of human KDM2A / FBXL11 (NP_036440.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Dog, Bat, Horse, Rabbit, Pig (100%).
Immunogen Type
Synthetic peptide
Immunogen
JHDM1A / KDM2A antibody was raised against synthetic peptide C-PRKDRQVHLTHFELE from an internal region of human KDM2A / FBXL11 (NP_036440.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Dog, Bat, Horse, Rabbit, Pig (100%); Mouse, Panda, Platypus (93%); Turkey (80%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-JHDM1A antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-JHDM1A antibody
ELISA (1:32000), IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Human Uterus lysates (calculated MW of 133kD according to NP_036440.1). Recommended concentration: 0.5-2 ug/ml.

Immunohistochemistry (IHC) of anti-JHDM1A antibody

Anti-JHDM1A / KDM2A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Goat Anti-JHDM1A Polyclonal Antibody – Cat# MBS248036

Western Blot (WB) of anti-JHDM1A antibody

KDM2A antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-JHDM1A Polyclonal Antibody, Western Blot (WB) – Cat# MBS248036

Other Notes

Small volumes of anti-JHDM1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for JHDM1A. It may not necessarily be applicable to this product.

NCBI GI #
16306580
NCBI GeneID
22992
NCBI Accession #
NP_036440.1
UniProt Primary Accession #
Q9Y2K7
UniProt Secondary Accession #
Q49A21; Q4G0M3; Q69YY8; Q9BVH5; Q9H7H5; Q9UK66; D4QA03; E9PIL6; I3VM55
UniProt Related Accession #
Q9Y2K7
GenBank Nucleotide #
NM_012308.2
NCBI Official Full Name
lysine-specific demethylase 2A isoform a
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 2A
NCBI Official Symbol
KDM2A
NCBI Official Synonym Symbols
FBL7; CXXC8; FBL11; FBXL11; JHDM1A; LILINA
UniProt Protein Name
Lysine-specific demethylase 2A
UniProt Synonym Protein Names
CXXC-type zinc finger protein 8; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; JmjC domain-containing histone demethylation protein 1A; [Histone-H3]-lysine-36 demethylase 1A
UniProt Gene Name
KDM2A
UniProt Synonym Gene Names
CXXC8; FBL7; FBXL11; JHDM1A; KIAA1004
UniProt Entry Name
KDM2A_HUMAN
NCBI Protein Information
lysine-specific demethylase 2A; F-box/LRR-repeat protein 11; CXXC-type zinc finger protein 8; [Histone-H3]-lysine-36 demethylase 1A; F-box and leucine-rich repeat protein 11; jumonji C domain-containing histone demethylase 1A; jmjC domain-containing histone demethylation protein 1A

NCBI Summary for JHDM1A

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
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UniProt Comments for JHDM1A

FBXL11: Histone demethylase that specifically demethylates 'Lys- 36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys- 36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. Belongs to the JHDM1 histone demethylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Demethylase; EC 1.14.11.27

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; nucleus

Molecular Function: unmethylated CpG binding; zinc ion binding; histone demethylase activity (H3-K36 specific)

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; double-strand break repair via nonhomologous end joining
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Research Articles on JHDM1A

1. KDM2A binds to the rDNA promoter with unmethylated CpG sequences via the CxxC-ZF domain

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-JHDM1A antibody

 Reference Product  PubMed Publications
 RELA antibody  >3 publications with JHDM1A and RELA
 BCL6 antibody  >1 publications with JHDM1A and BCL6

Pathways associated with anti-JHDM1A antibody

 Products by Pathway  Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 HDMs Demethylate Histones Pathway antibodies  HDMs Demethylate Histones Pathway Diagram

Diseases associated with anti-JHDM1A antibody

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >3 publications with JHDM1A and Lung Neoplasms
 Heart Failure Antibodies  >1 publications with JHDM1A and Heart Failure
 Nervous System Diseases Antibodies  >1 publications with JHDM1A and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >1 publications with JHDM1A and Neoplasms, Experimental
 Inflammation Antibodies  >1 publications with JHDM1A and Inflammation
 Necrosis Antibodies  >1 publications with JHDM1A and Necrosis

Organs/Tissues associated with anti-JHDM1A antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with JHDM1A and Lung
 Bone Antibodies  >2 publications with JHDM1A and Bone
 Mouth Antibodies  >2 publications with JHDM1A and Mouth
 Brain Antibodies  >2 publications with JHDM1A and Brain
 Prostate Antibodies  >1 publications with JHDM1A and Prostate
 Heart Antibodies  >1 publications with JHDM1A and Heart
 Embryonic Tissue Antibodies  >1 publications with JHDM1A and Embryonic Tissue
 Testis Antibodies  >1 publications with JHDM1A and Testis
 Blood Antibodies  >1 publications with JHDM1A and Blood
 Thymus Antibodies  >1 publications with JHDM1A and Thymus
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