• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SLC16A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248052

MBS248052 is an antibody targeting SLC16A2. Also known as Rabbit Polyclonal (IgG) to Human SLC16A2 / MCT8. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SLC16A2 / MCT8, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human SLC16A2 / MCT8
Product Gene Name
anti-SLC16A2 antibody
Product Synonym Gene Name
MCT8
Product Synonym Names
Anti-SLC16A2 / MCT8 Antibody (aa93-142) IHC-plus; SLC16A2; Allan-Herndon-Dudley syndrome; AHDS; DXS128E; DXS128; MCT8; Monocarboxylate transporter 7; Monocarboxylate transporter 8; MCT 7; MCT 8; MRX22; XPCT; MCT7; Human SLC16A2; MCT8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS, pH 7.4, 150mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Purity/Purification
Immunoaffinity purified
Concentration
1mg/ml (lot specific)
Specificity
SLC16A2/MCT8 antibody detects endogenous levels of SLC16A2.
Preparation and Storage
Store at -20°C.
Molecular Weight
Western Blot: Predicted MW 59 kDa
OMIM
gene 300523
3D Structure
ModBase 3D Structure for P36021
Immunogen Species
SLC16A2/MCT8 antibody was raised against Human.
Immunogen
SLC16A2/MCT8 antibody was raised against synthetic peptide from human SLC16A2. (AA Range: 93-142)
Epitope
aa93-142
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-SLC16A2 antibody

Family: Transporter; Subfamily: Monocarboxylic acid transporter
Applications Tested/Suitable for anti-SLC16A2 antibody
Immunohistochemistry - Paraffin (IHC), Western Blot (WB), Peptide ELISA
Application Notes for anti-SLC16A2 antibody
IHC - Paraffin (1:200)
Western Blot (1:500-1:100)
Peptide ELISA (1:10000)
(Optimal dilution to be determined by the researcher.)

Immunohistochemistry (IHC) of anti-SLC16A2 antibody

Anti-SLC16A2 / MCT8 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Rabbit Anti-SLC16A2 Polyclonal Antibody – Cat# MBS248052

Western Blot (WB) of anti-SLC16A2 antibody

Western blot of the lysates from HT-29 cells using SLC16A2 antibody.
Rabbit Anti-SLC16A2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248052

Other Notes

Small volumes of anti-SLC16A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SLC16A2. It may not necessarily be applicable to this product.

NCBI GI #
5730045
NCBI GeneID
6567
NCBI Accession #
NP_006508.1
UniProt Primary Accession #
P36021
UniProt Secondary Accession #
Q7Z797
UniProt Related Accession #
P36021
GenBank Nucleotide #
NM_006517.3
NCBI Official Full Name
monocarboxylate transporter 8
NCBI Official Synonym Full Names
solute carrier family 16, member 2 (thyroid hormone transporter)
NCBI Official Symbol
SLC16A2
NCBI Official Synonym Symbols
AHDS; MCT7; MCT8; XPCT; MCT 7; MCT 8; MRX22; DXS128; DXS128E
UniProt Protein Name
Monocarboxylate transporter 8
UniProt Synonym Protein Names
Monocarboxylate transporter 7; MCT 7; Solute carrier family 16 member 2; X-linked PEST-containing transporter
Protein Family
Monocarboxylate transporter
UniProt Gene Name
SLC16A2
UniProt Synonym Gene Names
MCT8; XPCT; MCT 8; MCT 7
UniProt Entry Name
MOT8_HUMAN
NCBI Protein Information
monocarboxylate transporter 8; monocarboxylate transporter 7; X-linked PEST-containing transporter

NCBI Summary for SLC16A2

This gene encodes an integral membrane protein that functions as a transporter of thyroid hormone. The encoded protein facilitates the cellular importation of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine (T2). This gene is expressed in many tissues and likely plays an important role in the development of the central nervous system. Loss of function mutations in this gene are associated with psychomotor retardation in males while females exhibit no neurological defects and more moderate thyroid-deficient phenotypes. This gene is subject to X-chromosome inactivation. Mutations in this gene are the cause of Allan-Herndon-Dudley syndrome. [provided by RefSeq, Mar 2012]
Show more

UniProt Comments for SLC16A2

SLC16A2: Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr. Defects in SLC16A2 are the cause of monocarboxylate transporter 8 deficiency (MCT8 deficiency); also known as Allan-Herndon-Dudley syndrome (AHDS). MCT8 deficiency consists of a severe form of X-linked psychomotor retardation combined with abnormal thyroid hormone (TH) levels. Thyroid hormone deficiency can be caused by defects of hormone synthesis and action, but it has also been linked to a defect in cellular hormone transport. Affected patients are males with abnormal relative concentrations of three circulating iodothyronines, as well as severe neurological abnormalities, including global developmental delay, central hypotonia, spastic quadriplegia, dystonic movements, rotary nystagmus, and impaired gaze and hearing. Heterozygous females had a milder thyroid phenotype and no neurological defects. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq13.2

Cellular Component: membrane; integral to plasma membrane; integral to membrane

Molecular Function: thyroid hormone transmembrane transporter activity; monocarboxylic acid transmembrane transporter activity; transporter activity; symporter activity

Biological Process: monocarboxylic acid transport; transport

Disease: Allan-herndon-dudley Syndrome
Show more

Research Articles on SLC16A2

1. two SNPs in MCT8 were related to circulating thyroid hormone levels in men but not in women: the rs5937843 polymorphism (G/T) was inversely associated with FT4 levels and the rs6647476 (T/C) polymorphism related negatively to circulating FT3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SLC16A2 antibody

 Products by Pathway  Pathway Diagram
 Thyroid Hormone Signaling Pathway antibodies  Thyroid Hormone Signaling Pathway Diagram

Diseases associated with anti-SLC16A2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >59 publications with SLC16A2 and Nervous System Diseases
 Neurobehavioral Manifestations Antibodies  >46 publications with SLC16A2 and Neurobehavioral Manifestations
 Allan-Herndon-Dudley syndrome Antibodies  >39 publications with SLC16A2 and Allan-Herndon-Dudley syndrome
 Brain Diseases Antibodies  >21 publications with SLC16A2 and Brain Diseases
 Congenital Abnormalities Antibodies  >9 publications with SLC16A2 and Congenital Abnormalities
 Abnormalities, Multiple Antibodies  >6 publications with SLC16A2 and Abnormalities, Multiple
 Fetal Growth Retardation Antibodies  >4 publications with SLC16A2 and Fetal Growth Retardation
 Seizures Antibodies  >3 publications with SLC16A2 and Seizures
 Liver Diseases Antibodies  >3 publications with SLC16A2 and Liver Diseases
 Weight Gain Antibodies  >3 publications with SLC16A2 and Weight Gain

Organs/Tissues associated with anti-SLC16A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >85 publications with SLC16A2 and Brain
 Liver Antibodies  >25 publications with SLC16A2 and Liver
 Kidney Antibodies  >12 publications with SLC16A2 and Kidney
 Pituitary Gland Antibodies  >9 publications with SLC16A2 and Pituitary Gland
 Placenta Antibodies  >8 publications with SLC16A2 and Placenta
 Embryonic Tissue Antibodies  >7 publications with SLC16A2 and Embryonic Tissue
 Heart Antibodies  >5 publications with SLC16A2 and Heart
 Vascular Antibodies  >5 publications with SLC16A2 and Vascular
 Connective Tissue Antibodies  >4 publications with SLC16A2 and Connective Tissue
 Bone Antibodies  >4 publications with SLC16A2 and Bone
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000