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CHRM4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248068

MBS248068 is an antibody targeting CHRM4. Also known as Rabbit Polyclonal (IgG) to Human CHRM4 / M4. Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$650 / 0.05 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CHRM4 / M4, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal (IgG) to Human CHRM4 / M4
Product Gene Name
anti-CHRM4 antibody
Product Synonym Names
CHRM4 Antibody, M4 muscarinic receptor Antibody, M4R Antibody, Muscarinic m4 receptor Antibody, HM4 Antibody, Muscarinic receptor m4 Antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol
Purity/Purification
Immunoaffinity Purified
Concentration
1 mg/mL (lot specific)
Specificity
CHRM4 Antibody detects endogenous levels of total CHRM4 protein.
Preparation and Storage
Store at -20°C for up to one year.
Molecular Weight
Western Blot: Predicted MW 53kDa
OMIM
118495
3D Structure
ModBase 3D Structure for P08173
Immunogen
CHRM4 / M4 antibody was raised against synthetic peptide from human CHRM4 (aa236-285).
Immunogen Species
CHRM4 / M4 antibody was raised against human
Epitope
aa236-285
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip

Product Categories/Family for anti-CHRM4 antibody

Family: GPCR; Subfamily: Acetylcholine
Applications Tested/Suitable for anti-CHRM4 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Peptide Enzyme-Linked Immunosorbent Assay
Application Notes for anti-CHRM4 antibody
IF: 1:100-1:500
IHC-P: 1:50
Optimal dilution to be determined by the researcher.

Immunohistochemistry (IHC) of anti-CHRM4 antibody

Anti-CHRM4 / M4 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Rabbit Anti-CHRM4 Polyclonal Antibody – Cat# MBS248068

Immunofluorescence (IF) of anti-CHRM4 antibody

Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit Anti-CHRM4 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS248068

Other Notes

Small volumes of anti-CHRM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHRM4. It may not necessarily be applicable to this product.

NCBI GI #
52426748
NCBI GeneID
1132
NCBI Accession #
NP_000732.2
UniProt Primary Accession #
P08173
UniProt Secondary Accession #
Q0VD60; Q4VBK7; B2RPP4
UniProt Related Accession #
P08173
GenBank Nucleotide #
NM_000741.2
NCBI Official Full Name
muscarinic acetylcholine receptor M4
NCBI Official Synonym Full Names
cholinergic receptor, muscarinic 4
NCBI Official Symbol
CHRM4
NCBI Official Synonym Symbols
HM4; M4R
UniProt Protein Name
Muscarinic acetylcholine receptor M4
Protein Family
Muscarinic acetylcholine receptor
UniProt Gene Name
CHRM4
UniProt Entry Name
ACM4_HUMAN
NCBI Protein Information
muscarinic acetylcholine receptor M4; acetylcholine receptor, muscarinic 4

NCBI Summary for CHRM4

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]
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UniProt Comments for CHRM4

mAChR M4: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 11p12-p11.2

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: G-protein coupled acetylcholine receptor activity

Biological Process: regulation of locomotion; cell proliferation; acetylcholine receptor signaling, muscarinic pathway; cell surface receptor linked signal transduction; muscarinic acetylcholine receptor, adenylate cyclase inhibiting pathway; signal transduction
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Research Articles on CHRM4

1. This study identify suppression of the PVHSIM1-->PAGvl/DR circuit connection as sufficient to induce overeating behavior.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CHRM4 antibody

 Reference Product  PubMed Publications
 CHRM2 antibody  >4 publications with CHRM4 and CHRM2

Pathways associated with anti-CHRM4 antibody

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies  Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram

Diseases associated with anti-CHRM4 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with CHRM4 and Nervous System Diseases
 Movement Disorders Antibodies  >1 publications with CHRM4 and Movement Disorders
 Arrhythmias, Cardiac Antibodies  >1 publications with CHRM4 and Arrhythmias, Cardiac
 Cognition Disorders Antibodies  >1 publications with CHRM4 and Cognition Disorders

Organs/Tissues associated with anti-CHRM4 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with CHRM4 and Eye
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