• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SPTAN1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248102

MBS248102 is an antibody targeting SPTAN1. Also known as Rabbit Polyclonal to Human SPTAN1 / Alpha Fodrin. Reactive in Human, Mouse. Host: Rabbit. More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
SPTAN1 / Alpha Fodrin, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human SPTAN1 / Alpha Fodrin
Product Gene Name
anti-SPTAN1 antibody
Product Synonym Names
Anti-SPTAN1 / Alpha Fodrin Antibody IHC-plus; SPTAN1; Alpha-II spectrin; Alpha Fodrin; Fodrin alpha chain; EIEE5; NEAS; Alpha-fodrin; Spectrin alpha chain; brain; SPTA2; Human SPTAN1; Alpha Fodrin
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Antiserum
Purity/Purification
Antiserum
Specificity
Human SPTAN1 / Alpha Fodrin
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Molecular Weight
282,282 Da
OMIM
gene 613477
3D Structure
ModBase 3D Structure for Q13813
Target Species
Human
Immunogen Description
Mixture of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2,400).
Immunogen Type
Recombinant protein
Immunogen
SPTAN1 / Alpha Fodrin antibody was raised against mixture of five recombinant constructs containing the entire C-terminal region of human alpha-II spectrin (amino acids 676-2,400). The 9th spectrin repeat also includes a Src-homology 3 domain, and the extreme C-terminus beyond the 20th spectrin repeat contains two EF hand calcium binding motifs. These constructs were expressed in and purified from E Coli.
Applications Tested/Suitable for anti-SPTAN1 antibody
Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes for anti-SPTAN1 antibody
ICC (1:500 - 1:1000), IF (1:500 - 1:1000), IHC-P (1:100), WB (1:5000 - 1:10000)
Usage: The serum can be diluted to 1:500-1:1000 for immunofluorescence staining and 1:5000-1:10000 for western blotting. On western blots look for a major band at 240kDa, depending on the species.

Immunohistochemistry (IHC) of anti-SPTAN1 antibody

Anti-SPTAN1 / Alpha Fodrin antibody IHC staining of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-SPTAN1 Polyclonal Antibody – Cat# MBS248102

Immunocytochemistry (ICC) of anti-SPTAN1 antibody

Mixed neuron-glial cultures stained with rabbit antibody to alpha-II spectrin (green) and mouse polyclonal antibody to Nestin (red). The alpha-II spectrin antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembranous cytoskeleton. Since alpha-II spectrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.
Rabbit Anti-SPTAN1 Polyclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS248102

Western Blot (WB) of anti-SPTAN1 antibody

Western blots of mouse sciatic nerve extract (lanes 1 and 2) and brain homogenate (3). A prominent band running at 240 kDa represents the intact alpha-II spectrin heavy chain. More minor bands below this likely represent in vivo proteolytic fragments of alpha-II spectrin.
Rabbit Anti-SPTAN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248102

Other Notes

Small volumes of anti-SPTAN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SPTAN1. It may not necessarily be applicable to this product.

NCBI GI #
154759259
NCBI GeneID
6709
NCBI Accession #
NP_003118.2
UniProt Primary Accession #
Q13813
UniProt Secondary Accession #
Q13186; Q15324; Q16606; Q59EF1; Q5VXV5; Q5VXV6; Q7Z6M5; Q9P0V0
UniProt Related Accession #
Q13813
GenBank Nucleotide #
NM_003127.3
NCBI Official Full Name
spectrin alpha chain, non-erythrocytic 1 isoform 2
NCBI Official Synonym Full Names
spectrin, alpha, non-erythrocytic 1
NCBI Official Symbol
SPTAN1
NCBI Official Synonym Symbols
NEAS; EIEE5; SPTA2
UniProt Protein Name
Spectrin alpha chain, non-erythrocytic 1
UniProt Synonym Protein Names
Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit
Protein Family
Spectrin
UniProt Gene Name
SPTAN1
UniProt Synonym Gene Names
NEAS; SPTA2
UniProt Entry Name
SPTN1_HUMAN
NCBI Protein Information
spectrin alpha chain, non-erythrocytic 1; alpha-fodrin; alpha-II spectrin; fodrin alpha chain; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit

NCBI Summary for SPTAN1

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
Show more

UniProt Comments for SPTAN1

SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: spectrin; microtubule cytoskeleton; membrane; intracellular membrane-bound organelle; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane

Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding

Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis

Disease: Epileptic Encephalopathy, Early Infantile, 5
Show more

Research Articles on SPTAN1

1. Variations in both alpha-spectrin (SPTA1) and beta-spectrin ( SPTB ) were found in a neonate with prolonged jaundice in a family where nine individuals had hereditary elliptocytosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SPTAN1 antibody

 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Interaction Between L1 And Ankyrins Pathway antibodies  Interaction Between L1 And Ankyrins Pathway Diagram

Diseases associated with anti-SPTAN1 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >10 publications with SPTAN1 and Brain Diseases
 Nervous System Diseases Antibodies  >10 publications with SPTAN1 and Nervous System Diseases
 Seizures Antibodies  >4 publications with SPTAN1 and Seizures
 Atrophy Antibodies  >3 publications with SPTAN1 and Atrophy
 Movement Disorders Antibodies  >3 publications with SPTAN1 and Movement Disorders
 Neurodegenerative Diseases Antibodies  >3 publications with SPTAN1 and Neurodegenerative Diseases
 EPILEPTIC ENCEPHALOPATHY, EARLY INFANTILE, 5 Antibodies  >2 publications with SPTAN1 and EPILEPTIC ENCEPHALOPATHY, EARLY INFANTILE, 5
 Precancerous Conditions Antibodies  >1 publications with SPTAN1 and Precancerous Conditions
 Adenocarcinoma Antibodies  >1 publications with SPTAN1 and Adenocarcinoma
 Lung Diseases Antibodies  >1 publications with SPTAN1 and Lung Diseases

Organs/Tissues associated with anti-SPTAN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with SPTAN1 and Brain
 Blood Antibodies  >4 publications with SPTAN1 and Blood
 Nerve Antibodies  >3 publications with SPTAN1 and Nerve
 Vascular Antibodies  >3 publications with SPTAN1 and Vascular
 Bladder Antibodies  >1 publications with SPTAN1 and Bladder
 Stomach Antibodies  >1 publications with SPTAN1 and Stomach
 Muscle Antibodies  >1 publications with SPTAN1 and Muscle
 Skin Antibodies  >1 publications with SPTAN1 and Skin
 Intestine Antibodies  >1 publications with SPTAN1 and Intestine
 Eye Antibodies  >1 publications with SPTAN1 and Eye
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000