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LDHB Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248188

MBS248188 is an antibody targeting LDHB. Also known as Goat Polyclonal to Human LDHB / LDH-B. Reactive in Gibbon, Chimpanzee, Horse, Human, Monkey, Orangutan. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
LDHB / LDH-B, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human LDHB / LDH-B
Product Gene Name
Product Synonym Gene Name
LDH-B
Product Synonym Names
Anti-LDHB / LDH-B Antibody (aa15-25) IHC-plus; LDHB; LDH heart subunit; Lactate dehydrogenase H chain; Lactate dehydrogenase B; LDH-B; TRG-5; LDH-H; LDHBD; Human LDHB; LDH-B
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Chimpanzee, Horse, Human, Monkey, Orangutan
Form/Format
Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human LDHB / LDH-B. Reported variants represent identical protein: NP_002291.1, NP_001167568.1.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
36,638 Da
OMIM
gene 614128
3D Structure
ModBase 3D Structure for P07195
Target Species
Human
Immunogen Description
Synthetic peptide C-EEATVPNNKIT from an internal region (near N-terminus) of human LDHB / LDH-B (NP_002291.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Dolphin, Horse (100%).
Immunogen Type
Synthetic peptide
Immunogen
LDHB / LDH-B antibody was raised against synthetic peptide C-EEATVPNNKIT from an internal region (near N-terminus) of human LDHB / LDH-B (NP_002291.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Dolphin, Horse (100%); Ferret, Bat, Elephant, Guinea pig (91%); Mouse, Sheep, Panda, Pig (82%); Rat (73%).
Antigen Modification
aa15-25
Applications Tested/Suitable for anti-LDHB antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-LDHB antibody
ELISA (1:4000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 35kD band observed in Human Heart and Kidney lysates (calculated MW of 36.6kD according to NP_002291.1). Recommended concentration: 0.3-1 ug/ml.

Immunohistochemistry (IHC) of anti-LDHB antibody

Anti-LDHB / LDH-B antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-LDHB Polyclonal Antibody – Cat# MBS248188

Western Blot (WB) of anti-LDHB antibody

LDHB antibody (0.3 ug/ml) staining of Human Heart (A) and Human Kidney (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-LDHB Polyclonal Antibody, Western Blot (WB) – Cat# MBS248188

Other Notes

Small volumes of anti-LDHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase B chain
NCBI Official Synonym Full Names
lactate dehydrogenase B
NCBI Official Symbol
LDHB
NCBI Official Synonym Symbols
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
UniProt Protein Name
L-lactate dehydrogenase B chain
UniProt Synonym Protein Names
LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46
UniProt Gene Name
LDHB
UniProt Synonym Gene Names
LDH-B; LDH-H
UniProt Entry Name
LDHB_HUMAN
NCBI Protein Information
L-lactate dehydrogenase B chain; LDH heart subunit; lactate dehydrogenase H chain; renal carcinoma antigen NY-REN-46; epididymis secretory protein Li 281

NCBI Summary for LDHB

This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2010]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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