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ACACB Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248196

MBS248196 is an antibody targeting ACACB. Also known as Goat Polyclonal to Mouse ACACB / ACC2. Reactive in Mouse, Human, Monkey, Rat, Bat. Host: Goat. More details

$680 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ACACB / ACC2, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Mouse ACACB / ACC2
Product Gene Name
anti-ACACB antibody
Product Synonym Gene Name
ACC2
Product Synonym Names
Anti-ACACB / ACC2 Antibody (Internal) IHC-plus; ACACB; ACCB; ACC-beta; ACC2; Acetyl-CoA carboxylase 2; Acetyl-CoA carboxylase beta; HACC275; Mouse ACACB; ACC2
Clonality
Polyclonal
Host
Goat
Species Reactivity
Mouse, Human, Monkey, Rat, Bat
Form/Format
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml (lot specific)
Specificity
Mouse ACACB / ACC2.
Preparation and Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
OMIM
601557
3D Structure
ModBase 3D Structure for O00763
Epitope
aa217-231
Immunogen Species
ACACB / ACC2 antibody was raised against Mouse.
Immunogen
Peptide with sequence C-TLNVQESGRVKYIKR, from the internal region of the protein sequence according to NP_598665.2.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-ACACB antibody
Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
Application Notes for anti-ACACB antibody
ELISA (1:16000), IHC-P (5 ug/ml), Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Optimal dilution to be determined by the researcher
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 277kD according to NP_001084.3. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product.

Other Notes

Small volumes of anti-ACACB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ACACB. It may not necessarily be applicable to this product.

NCBI GI #
134142062
NCBI GeneID
32
NCBI Accession #
NP_001084.3
UniProt Primary Accession #
O00763
UniProt Secondary Accession #
Q16852; Q1HEC1; Q6KE87; Q6KE89; Q6TY48; A6NK36
UniProt Related Accession #
O00763
GenBank Nucleotide #
NM_001093.3
NCBI Official Full Name
acetyl-CoA carboxylase 2
NCBI Official Synonym Full Names
acetyl-CoA carboxylase beta
NCBI Official Symbol
ACACB
NCBI Official Synonym Symbols
ACC2; ACCB; HACC275
UniProt Protein Name
Acetyl-CoA carboxylase 2
UniProt Synonym Protein Names
ACC-betaIncluding the following 1 domains:Biotin carboxylase (EC:6.3.4.14)
UniProt Gene Name
ACACB
UniProt Synonym Gene Names
ACC2; ACCB
UniProt Entry Name
ACACB_HUMAN
NCBI Protein Information
acetyl-CoA carboxylase 2; ACC-beta; acetyl-Coenzyme A carboxylase beta

NCBI Summary for ACACB

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms. [provided by RefSeq, Jul 2008]
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UniProt Comments for ACACB

ACC2: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Activity is increased by oligomerization. Activated by citrate. Citrate and MID1IP1 promote oligomerization. Inhibited by malonyl-CoA. ACC1 is the predominant form expressed in liver, adipocyte and mammary gland. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC2 is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid biosynthesis; Ligase; EC 6.4.1.2; Carbohydrate Metabolism - pyruvate; Mitochondrial; Carbohydrate Metabolism - propanoate; EC 6.3.4.14

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: mitochondrial outer membrane; mitochondrion; endomembrane system; nucleus; cytosol

Molecular Function: protein binding; acetyl-CoA carboxylase activity; metal ion binding; biotin carboxylase activity; ATP binding; biotin binding

Biological Process: response to drug; vitamin metabolic process; acetyl-CoA metabolic process; energy reserve metabolic process; positive regulation of cellular metabolic process; carnitine shuttle; cellular lipid metabolic process; response to organic cyclic substance; biotin metabolic process; negative regulation of fatty acid oxidation; fatty acid biosynthetic process; water-soluble vitamin metabolic process; protein homotetramerization
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Research Articles on ACACB

1. These data support a role for ACACB in obesity and potential roles for altered lipid metabolism in susceptibility to diabetic nephropathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACACB antibody

 Reference Product  PubMed Publications
 SREBF1 antibody  >6 publications with ACACB and SREBF1
 STK11 antibody  >4 publications with ACACB and STK11
 MLXIPL antibody  >2 publications with ACACB and MLXIPL
 FASN antibody  >2 publications with ACACB and FASN
 ACACA antibody  >2 publications with ACACB and ACACA
 PRKAA1 antibody  >1 publications with ACACB and PRKAA1
 CPT1A antibody  >1 publications with ACACB and CPT1A
 ACSS2 antibody  >1 publications with ACACB and ACSS2
 PRKAA2 antibody  >1 publications with ACACB and PRKAA2

Pathways associated with anti-ACACB antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Biotin Transport And Metabolism Pathway antibodies  Biotin Transport And Metabolism Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies  Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram

Diseases associated with anti-ACACB antibody

 Disease Name  Pubmed Publications
 Obesity Antibodies  >21 publications with ACACB and Obesity
 Fatty Liver Antibodies  >17 publications with ACACB and Fatty Liver
 Insulin Resistance Antibodies  >13 publications with ACACB and Insulin Resistance
 Liver Diseases Antibodies  >9 publications with ACACB and Liver Diseases
 Kidney Diseases Antibodies  >7 publications with ACACB and Kidney Diseases
 Inflammation Antibodies  >6 publications with ACACB and Inflammation
 Cardiovascular Diseases Antibodies  >5 publications with ACACB and Cardiovascular Diseases
 Heart Diseases Antibodies  >4 publications with ACACB and Heart Diseases
 Weight Loss Antibodies  >3 publications with ACACB and Weight Loss
 Necrosis Antibodies  >2 publications with ACACB and Necrosis

Organs/Tissues associated with anti-ACACB antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >23 publications with ACACB and Muscle
 Liver Antibodies  >22 publications with ACACB and Liver
 Heart Antibodies  >10 publications with ACACB and Heart
 Adipose Tissue Antibodies  >10 publications with ACACB and Adipose Tissue
 Connective Tissue Antibodies  >7 publications with ACACB and Connective Tissue
 Skin Antibodies  >2 publications with ACACB and Skin
 Kidney Antibodies  >2 publications with ACACB and Kidney
 Nerve Antibodies  >1 publications with ACACB and Nerve
 Thyroid Antibodies  >1 publications with ACACB and Thyroid
 Eye Antibodies  >1 publications with ACACB and Eye
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