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TGM4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248303

MBS248303 is an antibody targeting TGM4. Also known as Goat Polyclonal to Human TGM4 / Transglutaminase 4. Reactive in Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
TGM4 / Transglutaminase 4, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human TGM4 / Transglutaminase 4
Product Gene Name
Product Synonym Names
Anti-TGM4 / Transglutaminase 4 Antibody (aa49-63) IHC-plus; TGM4; Fibrinoligase; HTGP; TGase-4; Transglutaminase-4; Prostate transglutaminase; TGase P; TGP; Transglutaminase P; TG(P); Transglutaminase 4 (prostate); Human TGM4; Transglutaminase 4
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Chimpanzee, Gorilla, Human, Monkey, Orangutan
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human TGM4 / Transglutaminase 4.
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
77,145 Da
OMIM
600585
3D Structure
ModBase 3D Structure for P49221
Target Species
Human
Immunogen Description
Synthetic peptide C-NQPLQSYHQLKLEFS from an internal region (near N-terminus) of human TGM4 / Transglutaminase 4 (NP_003232.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Baboon (93%); Tamarin (80%).
Immunogen Type
Synthetic peptide
Immunogen
TGM4 / Transglutaminase 4 antibody was raised against synthetic peptide C-NQPLQSYHQLKLEFS from an internal region (near N-terminus) of human TGM4 / Transglutaminase 4 (NP_003232.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Baboon (93%); Tamarin (80%).
Antigen Modification
aa49-63
Applications Tested/Suitable for anti-TGM4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-TGM4 antibody
ELISA (1:16000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 75kD band observed in lysates of cell line HeLa (calculated MW of 77.1kD according to NP_003232.2). Recommended concentration: 1-3 ug/ml.

Immunohistochemistry (IHC) of anti-TGM4 antibody

Anti-TGM4 / Transglutaminase 4 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Goat Anti-TGM4 Polyclonal Antibody – Cat# MBS248303

Western Blot (WB) of anti-TGM4 antibody

TGM4 antibody (1 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-TGM4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248303

Other Notes

Small volumes of anti-TGM4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TGM4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein-glutamine gamma-glutamyltransferase 4
NCBI Official Synonym Full Names
transglutaminase 4
NCBI Official Symbol
TGM4
NCBI Official Synonym Symbols
TGP; hTGP
UniProt Protein Name
Protein-glutamine gamma-glutamyltransferase 4
UniProt Synonym Protein Names
Fibrinoligase; Prostate transglutaminase; Prostate-specific transglutaminase; Transglutaminase P; TG(P); TGP; TGase P; Transglutaminase-4; TGase-4
UniProt Gene Name
TGM4
UniProt Synonym Gene Names
TG(P); TGP; TGase P; TGase-4
UniProt Entry Name
TGM4_HUMAN
NCBI Protein Information
protein-glutamine gamma-glutamyltransferase 4; TG(P); TGase P; TGase-4; fibrinoligase; transglutaminase P; transglutaminase-4; prostate transglutaminase; transglutaminase 4 (prostate); prostate-specific transglutaminase

UniProt Comments for TGM4

TGM4: Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract. Belongs to the transglutaminase superfamily. Transglutaminase family.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 3p22-p21.33

Cellular Component: Golgi apparatus; extracellular matrix; cytoplasm

Molecular Function: protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: peptide cross-linking; mating plug formation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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