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Ferritin Heavy Chain / FTH1 (FTH1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248317

MBS248317 is an antibody targeting FTH1. Also known as Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1. Reactive in Human. Host: Rabbit. More details

$650 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ferritin Heavy Chain / FTH1, Polyclonal Antibody
Full Product Name
Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1
Product Gene Name
anti-FTH1 antibody
Product Synonym Names
Anti-Ferritin Heavy Chain / FTH1 Antibody (aa154-183) IHC-plus; FTH1; Apoferritin; Ferritin H subunit; Ferritin heavy chain; Ferritin; heavy polypeptide 1; FHC; FTHL6; PIG15; PLIF; FTH; Human Ferritin Heavy Chain; FTH1
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Protein A purified
Specificity
This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
OMIM
gene 615517
3D Structure
ModBase 3D Structure for P02794
Target
FTH1 / Ferritin Heavy chain
Immunogen Species
FTH1 / Ferritin Heavy chain antibody was raised against Human
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-FTH1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-FTH1 antibody
IHC - Paraffin (1:100)
Western blot (1:1000)
Flow Cytometry (1:10 - 1:50)
(Optimal dilution to be determined by the researcher)

Immunohistochemistry (IHC) of anti-FTH1 antibody

Anti-Ferritin Heavy Chain / FTH1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-FTH1 Polyclonal Antibody – Cat# MBS248317

Flow Cytometry (FC/FACS) of anti-FTH1 antibody

Flow cytometry of HepG2 cells using FTH1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-FTH1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS248317

Western Blot (WB) of anti-FTH1 antibody

Western blot of FTH1 Antibody (C-term) in A2058 cell line lysates (35 ug/lane). FTH1 (arrow) was detected using the purified Pab.
Rabbit Anti-FTH1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248317

Other Notes

Small volumes of anti-FTH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FTH1. It may not necessarily be applicable to this product.

NCBI GI #
56682959
NCBI GeneID
2495
NCBI Accession #
NP_002023.2
UniProt Primary Accession #
P02794
UniProt Secondary Accession #
Q3KRA8; Q3SWW1; B3KNR5
UniProt Related Accession #
P02794
GenBank Nucleotide #
NM_002032.2
NCBI Official Full Name
ferritin heavy chain
NCBI Official Synonym Full Names
ferritin, heavy polypeptide 1
NCBI Official Symbol
FTH1
NCBI Official Synonym Symbols
FHC; FTH; HFE5; PLIF; FTHL6; PIG15
UniProt Protein Name
Ferritin heavy chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 15 proteinFerritin heavy chain, N-terminally processed
Protein Family
Iron transporter
UniProt Gene Name
FTH1
UniProt Synonym Gene Names
FTH; FTHL6; Ferritin H subunit
UniProt Entry Name
FRIH_HUMAN
NCBI Protein Information
ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein

NCBI Summary for FTH1

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for FTH1

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; nucleus; cytosol

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: negative regulation of cell proliferation; receptor-mediated endocytosis; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5
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Research Articles on FTH1

1. FTH provides metabolic adaptation to tissue Fe overload. [Review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FTH1 antibody

 Reference Product  PubMed Publications
 FTL antibody  >12 publications with FTH1 and FTL
 TFRC antibody  >3 publications with FTH1 and TFRC
 FECH antibody  >2 publications with FTH1 and FECH
 ACO1 antibody  >1 publications with FTH1 and ACO1
 MAX antibody  >1 publications with FTH1 and MAX
 MYC antibody  >1 publications with FTH1 and MYC

Pathways associated with anti-FTH1 antibody

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 Clathrin Derived Vesicle Budding Pathway antibodies  Clathrin Derived Vesicle Budding Pathway Diagram
 Golgi Associated Vesicle Biogenesis Pathway antibodies  Golgi Associated Vesicle Biogenesis Pathway Diagram
 Iron Metabolism In Placenta Pathway antibodies  Iron Metabolism In Placenta Pathway Diagram
 Iron Uptake And Transport Pathway antibodies  Iron Uptake And Transport Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Mineral Absorption Pathway antibodies  Mineral Absorption Pathway Diagram
 Scavenging By Class A Receptors Pathway antibodies  Scavenging By Class A Receptors Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-FTH1 antibody

 Disease Name  Pubmed Publications
 Death Antibodies  >7 publications with FTH1 and Death
 Inflammation Antibodies  >5 publications with FTH1 and Inflammation
 Nervous System Diseases Antibodies  >5 publications with FTH1 and Nervous System Diseases
 Liver Diseases Antibodies  >4 publications with FTH1 and Liver Diseases
 Brain Diseases Antibodies  >4 publications with FTH1 and Brain Diseases
 Iron Overload Antibodies  >4 publications with FTH1 and Iron Overload
 Nerve Degeneration Antibodies  >2 publications with FTH1 and Nerve Degeneration
 Neurodegenerative Diseases Antibodies  >2 publications with FTH1 and Neurodegenerative Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with FTH1 and Carcinoma, Hepatocellular
 Edema Antibodies  >1 publications with FTH1 and Edema

Organs/Tissues associated with anti-FTH1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >13 publications with FTH1 and Blood
 Brain Antibodies  >9 publications with FTH1 and Brain
 Liver Antibodies  >8 publications with FTH1 and Liver
 Nerve Antibodies  >8 publications with FTH1 and Nerve
 Placenta Antibodies  >4 publications with FTH1 and Placenta
 Bone Antibodies  >4 publications with FTH1 and Bone
 Spleen Antibodies  >3 publications with FTH1 and Spleen
 Vascular Antibodies  >3 publications with FTH1 and Vascular
 Bone Marrow Antibodies  >3 publications with FTH1 and Bone Marrow
 Lung Antibodies  >3 publications with FTH1 and Lung
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