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SDF4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS248367

MBS248367 is an antibody targeting SDF4. Also known as Goat Polyclonal to Human SDF4. Reactive in Gibbon, Gorilla, Human, Monkey. Host: Goat. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
SDF4, Polyclonal Antibody
Full Product Name
Goat Polyclonal to Human SDF4
Product Gene Name
Product Synonym Names
Anti-SDF4 Antibody (Internal) IHC-plus; SDF4; 45 kDa calcium-binding protein; SDF-4; Stromal cell-derived factor 4; RP5-902P8.6; Stromal cell derived factor 4; Cab45; Human SDF4
Clonality
Polyclonal
Host
Goat
Species Reactivity
Gibbon, Gorilla, Human, Monkey
Form/Format
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Purity/Purification
Immunoaffinity Purified
Concentration
0.5 mg/ml (lot specific)
Specificity
Human SDF4. This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
Preparation and Storage
Store at -20 degree C. Minimize freezing and thawing.
Molecular Weight
39,608 Da
OMIM
614282
3D Structure
ModBase 3D Structure for Q9BRK5
Target Species
Human
Immunogen Description
Synthetic peptide C-EYKVKFLASKGHSEK from an internal region of human SDF4 (NP_057631.1; NP_057260.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Opossum (93%); Mouse, Rat, Hamster, Horse, Platypus (87%).
Immunogen Type
Synthetic peptide
Immunogen
SDF4 antibody was raised against synthetic peptide C-EYKVKFLASKGHSEK from an internal region of human SDF4 (NP_057631.1; NP_057260.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Opossum (93%); Mouse, Rat, Hamster, Horse, Platypus (87%); Goat, Panda, Dog, Bovine, Pig, Turkey, Chicken, Xenopus, Stickleback, Zebrafish (80%).
Antigen Modification
Internal
Applications Tested/Suitable for anti-SDF4 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SDF4 antibody
ELISA (1:64000), IHC-P (5 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 45kD band observed in lysates of cell line Jurkat (calculated MW of 41.8kD according to NP_057260.2). Recommended concentration: 0.1-0.3 ug/ml.

Immunohistochemistry (IHC) of anti-SDF4 antibody

Human, Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Goat Anti-SDF4 Polyclonal Antibody – Cat# MBS248367

Western Blot (WB) of anti-SDF4 antibody

SDF4 antibody (0.1 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SDF4 Polyclonal Antibody, Western Blot (WB) – Cat# MBS248367

Other Notes

Small volumes of anti-SDF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SDF4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
45 kDa calcium-binding protein isoform 2
NCBI Official Synonym Full Names
stromal cell derived factor 4
NCBI Official Symbol
SDF4
NCBI Official Synonym Symbols
Cab45; SDF-4
UniProt Protein Name
45 kDa calcium-binding protein
UniProt Synonym Protein Names
Stromal cell-derived factor 4; SDF-4
UniProt Gene Name
SDF4
UniProt Synonym Gene Names
CAB45; Cab45; SDF-4
UniProt Entry Name
CAB45_HUMAN
NCBI Protein Information
45 kDa calcium-binding protein

NCBI Summary for SDF4

This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities. [provided by RefSeq, Sep 2011]
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UniProt Comments for SDF4

SDF4: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: Golgi apparatus; membrane; Golgi lumen; cytoplasm; late endosome; plasma membrane

Molecular Function: identical protein binding; protein binding; calcium ion binding

Biological Process: fat cell differentiation; response to ethanol; UV protection; cerebellum development; calcium ion-dependent exocytosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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